A laboratory method for measuring the degree of attack byPhytophthora infestans

Umaerus, V.; Ihnell, D. I.

doi:10.1007/bf02361997

Cited by 25 publications

(5 citation statements)

References 4 publications

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“…This method is commonly used in many laboratories (Lapwood 1961, Umaerus andLihnell 1976;Sujkowski 1986;Dorrance and Inglis 1997;Kapsa 2001, andZarzycka 2001). However, stem infection is an important factor in late blight epidemics (Smith et al 1988;Bain et al 1996;Turkensteen 1996;Lammers et al 1998 andKapsa 2000), but there are very few studies on potato stem resistance (Dorrance and Inglis 1997;Kapsa 2001;Porter et al 2004 andMichalska et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Resistance of Potato to Stem Infection by Phytophthora infestans and a Comparison to Detached Leaflet and Field Resistance Assessments

et al. 2011

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To study the potato stem resistance to late blight, a new laboratory method has been established. Our aim was to understand relationships among resistance to P. infestans in stems and leaflets tested in laboratory and field resistance of plants. Sensitivity to P. infestans of stems and leaflets was investigated in 2004 and 2005 for fourteen cultivars, and in 2005 for fifteen breeding lines of various origins. We have performed a field assessment in 2005 for breeding lines and chosen cultivars. Laboratory tests were carried out twice a year, and their results correlated significantly. The correlation coefficients between leaflet and stem resistance data were significant and high (r ranged from 0.81 to 0.87). The correlation coefficient between Relative Area under the Disease Progress Curve (rAUDPC) values and stem resistance (r=−0.72) was higher than that between rAUDPC and leaflet resistance (r=−0.57). Laboratory assays for stem and detached leaflets resistance may be used for primary selection of breeding lines before their evaluation in field trials.Resumen Se ha establecido un nuevo método de laboratorio para estudiar la resistencia del tallo al tizón tardío. Nuestra meta fue entender las relaciones entre resistencia a P. infestans en tallos y folíolos probados en laboratorio y en resistencia de campo de plantas. La sensibilidad a P. infestans de tallos y folíolos se investigó en 2004 y 2005 para catorce variedades, y en 2005 para quince líneas mejoradas de varios orígenes. Desarrollamos un análisis de campo en 2005 para líneas de mejoramiento y variedades selectas. Las pruebas de laboratorio se llevaron a cabo dos veces al año, y sus resultados se correlacionaron significativamente. Los coeficientes de correlación entre los datos de resistencia de folíolos y tallo fueron significativos y altos (r fluctuó de 0.81 a 0.87). El coeficiente de correlación entre los valores del Área Relativa bajo la Curva de Progreso de la Enfermedad (rAUDPC) y la resistencia de tallo (r=−0.72) fue más alta que la de entre rAUDPC y la resistencia del folíolo (r=−0.57). Los ensayos de laboratorio de resistencia de tallo y de folíolos separados pudiera usarse para una selección primaria de líneas de mejoramiento antes de su evaluación en ensayos de campo.

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Section: Discussionmentioning

confidence: 99%

Resistance of Potato to Stem Infection by Phytophthora infestans and a Comparison to Detached Leaflet and Field Resistance Assessments

et al. 2011

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“…The molecular isolation of R genes is a prerequisite to understand the biochemical mechanism underlying this interaction and to unveil their coevolutionary pattern that may have implications on the strategy of R gene deployment in dis-ease management. Several methods have been described to evaluate foliar late blight resistance, including field tests, whole-plant greenhouse assays (18), and laboratory tests on detached leaves (10,24), leaflets (11,20), or leaf disks (7). Recently, three R genes, namely R1 (1), R3a (8), and RB/Rpi-blb1 (15,22), have been cloned.…”

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confidence: 99%

An Accurate In Vitro Assay for High-Throughput Disease Testing of Phytophthora infestans in Potato

et al. 2005

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Huang, S., Vleeshouwers, V. G. A. A., Visser, R. G. F., and Jacobsen, E. 2005. An accurate in vitro assay for high-throughput disease testing of Phytophthora infestans in potato. Plant Dis. 89:1263-1267.An in vitro inoculation assay was developed as a routine disease testing method to study genefor-gene interactions in the potato (Solanum tuberosum)-Phytophthora infestans pathosystem. The specificity and reliability of the new method was compared with the well-established detached-leaf assay. Four P. infestans isolates were tested for avirulence on a set of R gene differentials using tissue cultured plantlets and detached leaves. Both methods gave identical conclusions on avirulence profiles of all isolates. A population of 93 clones was phenotyped for segregation of two closely linked and functionally distinct genes-R3a and R3b-in the R3 locus. Both methods resulted in phenotypic scorings that were in perfect agreement for all clones. Furthermore, the phenotyping of the population was fully consistent with the genotyping obtained from analysis of molecular markers that flank each gene. This new assay is quick, space-effective, and accurate and can be used for investigation of the qualitative interaction between potato and P. infestans.

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“…On the 40thday of planting, the terminal leaflet of the fourth leaf from the top was used in the study. Five leaflets collected from each cultivar were placed in plastic trays on perforated plastic separators (Umaerus and Lihnell, 1976) and inoculated with 50 µl zoosporangial suspension containing 6x10 4 zoospores/ml using an autopipette. Trays containing the inoculated leaflets were incubated in an incubator at 18+1 o C. Lesion size (length and width) was measured after every24h inoculation and the lesion area was calculated by using the following formula (Singh and Bhattacharyya, 1995).…”

Section: 3detached Leaf Methods /Pathogen Inoculationmentioning

confidence: 99%

A Quantative Real-Time PCR of Phytophthora infestans in different Indian potato cultivars

Hussain¹,

Anwar²

2013

IOSR-JAVS

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A laboratory method for measuring the degree of attack byPhytophthora infestans

Cited by 25 publications

References 4 publications

Resistance of Potato to Stem Infection by Phytophthora infestans and a Comparison to Detached Leaflet and Field Resistance Assessments

Resistance of Potato to Stem Infection by Phytophthora infestans and a Comparison to Detached Leaflet and Field Resistance Assessments

An Accurate In Vitro Assay for High-Throughput Disease Testing of Phytophthora infestans in Potato

A Quantative Real-Time PCR of Phytophthora infestans in different Indian potato cultivars

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