A laboratory evaluation of the antibacterial and cytotoxic effect of Liquorice when used as root canal medicament

Abstract

Liquorice extract either separately or as Liquorice/Ca(OH)(2) mixture had a potent bactericidal effect against Enterococcus faecalis and retained compatibility with fibroblasts in tissue culture compared to the commonly used root canal medicament Ca(OH)(2).

Evaluation of antimicrobial efficacy of cetrimide and <i>Glycyrrhiza glabra L.</i> extract against <i>Enterococcus faecalis</i> biofilm grown on dentin discs in comparison with NaOCl

“…Flavonoids, the other components of G. glabra extract are also responsible for the antimicrobial activity by inhibiting the cell membrane electron transport chain 24) . As well as the antimicrobial activity of saponins, lowering the surface tension was also reported 13,23) . Glycyrrhiza glabra extract containing 7-7.5% glycyrrhizin was found advantageous in terms of cytotoxicity because 87% of periodontal ligament fibroblasts were alive after 48 h 13) .…”

“…As well as the antimicrobial activity of saponins, lowering the surface tension was also reported 13,23) . Glycyrrhiza glabra extract containing 7-7.5% glycyrrhizin was found advantageous in terms of cytotoxicity because 87% of periodontal ligament fibroblasts were alive after 48 h 13) . The effective concentrations of NaOCl (0.05-6%) and cetrimede (0.2-7.5%) in endodontic irrigation are well known, wheras the concentration of the G glabra L. extract is not reported 7,18,25,26) .…”

“…However, a weakness of this model is that the biofilms on dentin discs are directly exposed to the antimicrobial agents, which differs from their exposure during root canal irrigation. The test microorganism was chosen as E. faecalis, since it is known to be associated with failed root canal treatments, and its ATCC 29212 strain was used due to its ability to form biofilms 6,7,13,18) . Antimicrobial agents have to be able to penetrate into the extracellular matrix or to break of the bonds in the biofilm to eliminate the microorganisms.…”

“…Glycyrrhizin is a tripertenic saponin and comprises the major component of Glycyrrhiza glabra L. It demonstrated anti-inflammatory, antimicrobial, anticarcinogenic and hepatoprotective effects 9) . In dental literature, G. glabra L. showed a markedly inhibition of bacterial growth and adherence of cariogenic Streptococcus mutans and E. Faecalis [11][12][13] . The aim of this study was to determine the antimicrobial efficacy of cetrimide and G. glabra L. extract against E. faecalis biofilms grown on dentine discs by using the agents at their respective minimal bactericidal concentrations (MBCs) for three different exposure durations in comparison with NaOCl.…”

Antibacterial effect of chlorhexidine-cetrimide combination, <i>Salvia officinalis</i> plant extract and octenidine in comparison with conventional endodontic irrigants

“…Most of the irrigation solutions commonly used in Antibacterial effect of chlorhexidine-cetrimide combination, Salvia officinalis plant extract and octenidine in comparison with conventional endodontic irrigants endodontic treatment still have a number of issues, which means that an alternative solution with good antimicrobial activity, less toxicity and better tissue dissolving properties is still being researched. The role of natural extracts for endodontic purpose has been researched for plants such as Arctium lappa, Morinda citrifolia, Triphala, Green Tea Polyphenols, Liquorice, Ocimum sanctum, Cinnamomum zeylanicum and Syzygium aromaticum due to their antimicrobial efficacy against E. faecalis [22][23][24][25][26] . Salvia officinalis, commonly known as sage, belongs to the Lamiaceae (Labiatae) family.…”