A Labile Serum Factor Clotting Defect: Its Demonstration by the Thromboplastin Generation Test and Its Clinical Significance*

Connor, William E.; Warner, E. D.; Carter, John R.

doi:10.1172/jci104225

Cited by 10 publications

(5 citation statements)

References 17 publications

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“…Less readily distinguishable is TVF from the recently described labile serum fraction of Connor, Warner, and Carter (43), an entity required for normal thromboplastin generation, and with properties remarkably similar to some of those of TVF. Abnormality in this substance was observed in some subjects with unexplained hemorrhagic diathesis, in the newborn, in bishydroxycoumarin (Dicumarol)-treated subjects, and in patients with liver disease.…”

Section: Resultsmentioning

confidence: 94%

The Effect of Lanthanides and Actinides on Blood Coagulation. I. Evidence for and Properties of a New Serum Thromboplastic Factor*

Colman¹,

Alexander²

1964

J. Clin. Invest.

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Section: Resultsmentioning

confidence: 94%

The Effect of Lanthanides and Actinides on Blood Coagulation. I. Evidence for and Properties of a New Serum Thromboplastic Factor*

Colman¹,

Alexander²

1964

J. Clin. Invest.

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“…factor and hemoglobinopathy patients, Connor et al (9) reported a series of patients with "labile serum factor." Table 2 summarizes a comparison of the reported serum defects.…”

Section: Discussionmentioning

confidence: 99%

“…Both Henstell et al (15) and Connor e t al. (9) reported correction of the defect by the addition of dilute thrombin t o the reaction mixture and failure of heated (thrombin-inacti- Platelet aggregation studies in the Virginia family revealed a qualitative platelet defect. Similar studies were not performed on the Car.…”

Section: Discussionmentioning

confidence: 99%

“…Of tbe officially recognized plasma coagulation factors, serum defect without plasma defect is produced only by deficiencies of factor IX or X. Various serum defects have been reported in persons with normal levels of these two factors who d o not appear t o have inheritable bleeding disorders (2,9,15). In 1957, Chirico and McElfresh (8) reported a familial abnormality of thromboplastin generation detected in the serum component of the TGT.…”

Section: Extractmentioning

confidence: 99%

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“Car. Factor” Deficiency Revisited

Komp

1975

Pediatr Res

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“…Autocatalysis is one of the best known biochemical amplifiers and constitutes the amplification needed in the development of thrombin activity. Repeated refusals to pay attention to the importance role of thrombin also accounts for the "long" life of the plasma thromboplastin concept (18) which no one can accept once it is seen that the thromboplastin generation test does not work unless thrombin is is in the "plasma thromboplastin" generation mixture (16,19). This hypo-thetical substance is not placed in the two above schemes, but does still appear in the one by Horowitz 7, and the 1962 report of Ratnoff and Davie (39).…”

Section: Surface Contactmentioning

confidence: 99%

Basic Enzymology of Blood Coagulation*

1965

Thromb Haemost

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SummaryI have considered the accuracy of the “enzyme cascade” sequence or “waterfall sequence” for blood clotting proposed recently. My remarks are not intended to be exhaustive, but rather representative of a perspective. I regard these presentations as being at the speculative level of science, created with a wild use of the imagination. Some of the clotting factor precursors that are supposed to be converted in succession to active enzymes may not exist and certainly are not known to be enzymes. I find it against my sense of order to believe that six proteolytical enzymes are needed to get thrombin. That would require six special sites in proteins where each enzyme does its work. Each enzyme would not split the other five sites if located as single or multiple frequencies in the five other substrates. While creating hypothetical enzymes and assigning Roman numerals to them the well documented function of the best known enzyme; namely, thrombin is disregarded. The capacity of prothrombin to form derivatives is disregarded. This immediately places the superstructure on a false base. The sequence is definitely not in accurate order. There is no elaborate mobilization of chemical events on the periphery of prothrombin which leads to doing something to prothrombin. The second known enzyme, autoprothrombin C, is included in the plasma-platelet system (intrinsic) whereas its main, if not exclusive place, is in the conversion of prothrombin to thrombin when tissue-plasma-platelet mechanisms are in operation. What is meant by t he term prothrombin is not clarified, and the way in which the required phospholipid originates is not accounted for. If there is a factor XII it is not the exclusive way to create conditions that will initiate prothrombin activation in the plasma-platelet system. Most of the steps postulated could only be accurate if it were possible to deny important conclusions stated in papers which are not at all considered. Evidently refutation of facts in these papers was not possible and a parochial kind of authority was promoted1 ) on the basis of “consistent with most of the current investigations”. I like to recal11 ) the words of Henry David Thoreau: “Any man more right than his neighbor, constitutes a majority of one.” The advances in prothrombin chemistry are sufficiently substantial for the formulation of a conceptual theme consistent with principles of enzymology, and as I have indicated above, can also serve as a clarification of the proposed cascade or waterfall diagrams.

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A Labile Serum Factor Clotting Defect: Its Demonstration by the Thromboplastin Generation Test and Its Clinical Significance*

Cited by 10 publications

References 17 publications

The Effect of Lanthanides and Actinides on Blood Coagulation. I. Evidence for and Properties of a New Serum Thromboplastic Factor*

The Effect of Lanthanides and Actinides on Blood Coagulation. I. Evidence for and Properties of a New Serum Thromboplastic Factor*

“Car. Factor” Deficiency Revisited

Basic Enzymology of Blood Coagulation*

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