A label-free SPR biosensor for specific detection of TLR4 expression; introducing of 10-HDA as an antagonist

Eslami-Kaliji, Farshid; Mirahmadi-Zare, Seyede Zohreh; Nazem, Saeid; Shafie, Negar; Ghaedi, Rassoul; Asadian-Esfahani, Mohammad Hossein

doi:10.1016/j.ijbiomac.2022.07.035

Cited by 11 publications

(6 citation statements)

References 34 publications

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“…Finally, the hydrophobic 10-HDA was loaded on the core–shell nanocomposite by hydrogen bonding interactions to obtain TFH@TA/AuNPs-HDA. 10-HDA is a medicinal ingredient of RJ and has anti-inflammatory, immunomodulatory, and anti-arthritic effects. ,− Therefore, 10-HDA is considered as an effective drug for RA treatment, and its corporation with other nanocomposite components increases the anti-inflammatory and anti-arthritic efficiency.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Immunomodulation and Tissue Protection on the Rheumatoid Arthritis Models Using a Tragacanth Frankincense-Based Core–Shell Nanostructure

Keshtiara

Mirahmadi-Zare

Hadadzadeh

et al. 2023

ACS Appl. Nano Mater.

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Rheumatoid arthritis (RA) is a chronic autoimmune disease that requires anti-inflammatory drugs, analgesics, and tissue protectors. To deal with this disease, the pain and inflammation should be relieved in the first stage, and tissue protectors should be used to restore joint damage that limits movement. Therefore, a comprehensive multifunctional core–shell nanocomposite (TFH@TA/AuNPs-HDA) with a continuous anti-inflammatory effect and a special tissue protection potential in the core was designed. Here, the hydrothermal reaction was applied to synthesize the hydrogel core from tragacanth and frankincense gums; then, the shell was self-assembled with tannic acid/gold nanoparticles (TA/AuNPs) and 10-hydroxy-2-decanoic acid (10-HDA). The biocompatibility, anti-inflammatory properties, and tissue protective effect of TFH@TA/AuNPs-HDA were investigated on RAW264.7 cells and in collagen-induced RA rat models. Moreover, the molecular docking demonstrated the interactions of 10-HDA and AuNPs with NF-κB signaling pathway proteins, confirming the anti-inflammatory effect of 10-HDA and AuNP suppression of the NF-κB pathway. The results revealed that TFH@TA/AuNPs-HDA could be an appropriate system for RA therapy due to the synergistic alleviation of inflammation and joint tissue destruction.

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Section: Resultsmentioning

confidence: 99%

Simultaneous Immunomodulation and Tissue Protection on the Rheumatoid Arthritis Models Using a Tragacanth Frankincense-Based Core–Shell Nanostructure

Keshtiara

Mirahmadi-Zare

Hadadzadeh

et al. 2023

ACS Appl. Nano Mater.

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“…One of these receptors is TLR4, which serves as a sensor for damage-associated molecular patterns [ 141 ]. Farshid et al found that 10-HDA acts as an antagonist to inhibit immune cell activation induced by TLR4 [ 142 ]. These findings underscore the immunomodulatory properties of 10-HDA and its potential role in regulating immune responses.…”

Section: Properties Of 10-hydroxy-2-decenoic Acidmentioning

confidence: 99%

Metabolic Profile of Alzheimer’s Disease: Is 10-Hydroxy-2-decenoic Acid a Pertinent Metabolic Adjuster?

Gong,

Luo,

et al. 2023

Metabolites

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Alzheimer’s disease (AD) represents a significant public health concern in modern society. Metabolic syndrome (MetS), which includes diabetes mellitus (DM) and obesity, represents a modifiable risk factor for AD. MetS and AD are interconnected through various mechanisms, such as mitochondrial dysfunction, oxidative stress, insulin resistance (IR), vascular impairment, inflammation, and endoplasmic reticulum (ER) stress. Therefore, it is necessary to seek a multi-targeted and safer approach to intervention. Thus, 10-hydroxy-2-decenoic acid (10-HDA), a unique hydroxy fatty acid in royal jelly, has shown promising anti-neuroinflammatory, blood–brain barrier (BBB)-preserving, and neurogenesis-promoting properties. In this paper, we provide a summary of the relationship between MetS and AD, together with an introduction to 10-HDA as a potential intervention nutrient. In addition, molecular docking is performed to explore the metabolic tuning properties of 10-HDA with associated macromolecules such as GLP-1R, PPARs, GSK-3, and TREM2. In conclusion, there is a close relationship between AD and MetS, and 10-HDA shows potential as a beneficial nutritional intervention for both AD and MetS.

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“…Поверхневий плазмонний резонанс -це резонансне коливання електронів на межі розділу між середовищами із негативною та позитивною діелектричною проникністю, стимульоване зовнішнім електромагнітним (ЕМ) лазерним випромінюванням [14,15]. Умовою збудження плазмону є від'ємна дійсна частина діелектричної проникності металу та рівність У оптичній конфігурації Кречмана (рис.…”

Section: модель кречманаunclassified

Effect of the Thickness of Nanoscale Layers of the Metal-Dielectric Structure on Its Optical Characteristics

Hetmanchuk

Machulianslyi

2022

Microsyst., Electron. & Acoust.

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A biosensor is a device which uses a mixture of biological and physicochemical components to detect molecular-level interactions. Essentially, biosensors detect the change in physical properties which occur when an immobilized biological molecule, such as an antibody or a protein, binds to their target analyte. A number of methods have been developed to determine interactions between bioreceptors and their analytes, many of which are based on the principle of Surface Plasmon Resonance, or SPR. The ubiquity of SPR comes from its inherent advantages; it allows real time, highly sensitive monitoring of a wide range of molecular interactions, without the requirement for additional labelling of samples. Surface Plasmon Resonance is caused by the interaction of electromagnetic radiation and the free-flowing cloud of electrons within a metal, a process which induces electromagnetic waves and, under certain conditions, resonance effects. Alterations in the physical conditions at the surface have a significant effect on this resonance, and the detection of these changes form the basis of the SPR biosensor. In the work, the effect of the thickness of the layers of the metal-dielectric structure on its optical characteristics was studied. The simulation was carried out for the Krechman configuration, which is a glass prism on which a multilayer metal-dielectric structure is applied. AuSiO2 was used as a metal-dielectric structure, and a laser beam with a wavelength of 633 nm was used as a source of external electromagnetic radiation. The thickness of the gold film varied from 1 to 60 nanometers. The lowest value of the reflection coefficient is reached at a thickness of 50 nm. The thickness of the silicon dioxide film varied from 1 to 30 nm. When the thickness of the dielectric layer is up to 10 nm, its influence on the optical characteristics is insignificant. Macroscopic values of parameters were used in the study, but it is known that real parameters differ greatly from macroscopic ones. To increase the accuracy of modeling the sensor element in further studies, it will be necessary to take into account dimensional changes in the optical parameters of the nanosized metal component in the structure of the sensor element.

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A label-free SPR biosensor for specific detection of TLR4 expression; introducing of 10-HDA as an antagonist

Cited by 11 publications

References 34 publications

Simultaneous Immunomodulation and Tissue Protection on the Rheumatoid Arthritis Models Using a Tragacanth Frankincense-Based Core–Shell Nanostructure

Simultaneous Immunomodulation and Tissue Protection on the Rheumatoid Arthritis Models Using a Tragacanth Frankincense-Based Core–Shell Nanostructure

Metabolic Profile of Alzheimer’s Disease: Is 10-Hydroxy-2-decenoic Acid a Pertinent Metabolic Adjuster?

Effect of the Thickness of Nanoscale Layers of the Metal-Dielectric Structure on Its Optical Characteristics

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