A label-free RTP sensor based on aptamer/quantum dot nanocomposites for cytochrome <i>c</i> detection

Li, Dongxia; Guo, Jing; Zhao, Liang; Zhang, Guoxian; Yan, Guiqin

doi:10.1039/c9ra05761g

Cited by 7 publications

(3 citation statements)

References 51 publications

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“…It can be seen that the method has good sensitivity, which is comparable to many fluorescence-methods. [17,22,23,[28][29][30]…”

Section: Analytical Figures Of Cyt Cmentioning

confidence: 99%

Luminescent gold nanoclusters as a signal reporter for cytochrome c assay with a double signal amplification strategy

Dou

2022

J Chinese Chemical Soc

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Quantitative determination of serum cytochrome c (Cyt c) is of importance in the regular medical assessment of cancer diseases. This work reported a new fluorescence method for the determination of Cyt c with glutathione‐protected gold nanoclusters (GSH‐AuNCs) as a signal reporter. Cyt c was observed to quench the fluorescence of GSH‐AuNCs due to its electron‐rich structure. This inhibitory effect was further strengthened by its peroxidase mimetic catalytic activity on hydrogen peroxide (H2O2)‐mediated oxidation of GSH‐AuNCs. The inhibitory efficiency was linearly related to Cyt c concentrations ranging from 10.0 to 700.0 nM with a limit of detection of 2.7 nM (3sb/S). The relative standard deviation was 3.6% for Cyt c at a 40.0 nM concentration level (n = 11). The practical application of the method was evaluated by the determination of Cyt c in spiked human serum samples. The recoveries were within the range from 92.6 to 104.8%, suggesting its potential application in biomedical and clinical diagnosis.

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“…It can be seen that the method has good sensitivity, which is comparable to many fluorescence-methods. [17,22,23,[28][29][30]…”

Section: Analytical Figures Of Cyt Cmentioning

confidence: 99%

Luminescent gold nanoclusters as a signal reporter for cytochrome c assay with a double signal amplification strategy

Dou

2022

J Chinese Chemical Soc

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“…Some studies have focused on detecting several targets based on the aptamer-competitive quenching of carbon quantum dots and graphene materials [ 120 , 121 , 122 , 123 , 124 , 125 ]. Recently, GO was used as a “nanoquencher” for fluorescent materials via the FRET strategy with ssDNA as a nanoscaffold, capable of adsorbing to the GO surface through π–π stacking interactions with the nucleotide bases [ 126 , 127 , 128 , 129 ].…”

Section: Fluorescence Aptasensormentioning

confidence: 99%

Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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“…Protein detections are carried out in a wide variety of ways that are based on specific recognition of biomarkers using specific antibodies or aptamers, gel electrophoresis, surface-enhanced laser desorption–ionization mass spectrometry, and so on. − While fluorescent or chemiluminescent labeled specific antibodies are commonly utilized in sensitive protein detection methods, their high fabrication cost poses significant constraints on their widespread applications. Recent advancements have focused on the development of aptamer-based fluorescent and electrochemical methods for protein detection. − Yet, finding specific aptamers for each protein analyte remains a formidable and time-consuming challenge. There is a growing demand for the development of protein sensing probes that can be easily synthesized at a low cost and applied across a range of protein analytes with great control over their sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

Dual Optical Response Strategy for the Detection of Cytochrome c Using Highly Luminescent Lanthanide-Based Nanotubular Sensor Arrays

Kamalakshan,

Jamuna,

Chittilappilly Devassy

et al. 2024

ACS Appl. Bio Mater.

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Here, we demonstrate a label-free dual optical response strategy for the detection of cytochrome c (Cyt c) with ultrahigh sensitivity using highly luminescent lanthanides containing inorganic−organic hybrid nanotubular sensor arrays. These sensor arrays are formed by the sequential incorporation of the photosensitizers 2,3-dihydroxynaphthalene (DHN) or 1,10-phenanthroline (Phen), and trivalent lanthanide terbium ions (Tb 3+ ) into sodium lithocholate (NaLC) nanotube templates. Our sensing platform relies on the detection and quantification of Cyt c in solution by providing dual photoluminescence quenching responses from the nanotubular hybrid arrays in the presence of Cyt c. The large quenching of the sensitized Tb 3+ emission within the DHN/Phen-Tb 3+ -NaLC nanotubular sensor arrays caused by the strong binding of the photosensitizers to Cyt c provides an important signal response for the selective detection of Cyt c. This long-lived lanthanide emission response-based sensing strategy can be highly advantageous for the detection of Cyt c in a cellular environment eliminating background fluorescence and scattering signals through time-gated measurements. The DHN containing nanotubular sensor arrays (DHN-NaLC and DHN-Tb 3+ -NaLC) provide an additional quenching response characterized by a unique spectral valley splitting with quantized quenching dip on the DHN fluorescence emission. This spectral quenching dip resulting from efficient FRET between the protein bound DHN photosensitizer and the heme group of Cyt c serves as an important means for specific detection and quantification of Cyt c in the concentration range of 0−30 μM with a low detection limit of around 20 nM.

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A label-free RTP sensor based on aptamer/quantum dot nanocomposites for cytochrome c detection

Abstract: In this study, the nanocomposites from polyethyleneimine-capped Mn-doped ZnS QDs (PEI-QDs) and Cyt c binding aptamer (CBA) were prepared and used as Cyt c RTP sensors..

Cited by 7 publications

References 51 publications

Luminescent gold nanoclusters as a signal reporter for cytochrome c assay with a double signal amplification strategy

Luminescent gold nanoclusters as a signal reporter for cytochrome c assay with a double signal amplification strategy

Recent Advances in Aptamer Sensors

Dual Optical Response Strategy for the Detection of Cytochrome c Using Highly Luminescent Lanthanide-Based Nanotubular Sensor Arrays

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