A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity

Wen, Jiahui; Liu, Yongming; Li, Jingwen; Hao, Lin; Zheng, Yiran; Yan, Chen; Chen, Lingxin

doi:10.1039/d0an00060d

Cited by 7 publications

(1 citation statement)

References 63 publications

(31 reference statements)

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“…Besides traditional radioactive labeling and enzyme-linked immunosorbent assay (ELISA) [15,16], a series of novel sensing strategies based on photochemistry have been developed for nuclease detection. For example, conjugated polymers, gold nanoparticles and G-quadruplex-based DNAzyme have provided colorimetric assay of nuclease [17][18][19][20][21]. Additionally, fluorescence-based nuclease assay has been increasingly concerned to increase sensitivity [22].…”

Section: Introductionmentioning

confidence: 99%

General Gel-sol Method to Synthesize Various Highly Fluorescent Nanoclusters and Assay of Nuclease with the Near Infrared-emitting Gold Nanoclusters

Xia

Luo

2023

J Fluoresc

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A general egg white gel-sol strategy for fabrication of highly fluorescent Au, Ag, Cu and Pt nanoclusters (NCs) and the first example of using Au NCs for assay of nuclease activity and inhibition were described. The Au NCs enabled bright red fluorescence, and the other Ag, Cu and Pt NCs have highly blue emission. The red-emitting Au NCs were further applied in assay of S1 nuclease activity and inhibition. Free hemin efficiently quenches the emission of Au NCs by photoinduced electron transfer due to formation of Au NCs-hemin conjugates. However, G-quadruplex/hemin exerts negligible effect on its fluorescence due to no Au NCs-hemin conjugate formed. There are stronger electrostatic repulsion effects between both negatively charged G-quadruplex and Au NCs. Therefore, a novel Gquadruplex/hemin-based Au NCs fluorescent sensor for S1 nuclease was designed. A known G-rich oligonucleotide (ODN) serves as not only substrate for S1 nuclease but also for the construction of Gquadruplex/hemin. The G-rich ODN is hydrolyzed into fragments by S1 nuclease resulting in no Gquadruplex/hemin formation. Therefore, the free hemin quenches Au NCs fluorescence remarkably and the assay of S1 nuclease activity and inhibition has accomplished. Both the fluorescent NCs syntheses and the detection of S1 nuclease are facile and efficient.

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Section: Introductionmentioning

confidence: 99%

General Gel-sol Method to Synthesize Various Highly Fluorescent Nanoclusters and Assay of Nuclease with the Near Infrared-emitting Gold Nanoclusters

Xia

Luo

2023

J Fluoresc

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Polydiacetylene vesicles acting as colorimetric sensor for the detection of plantaricin LD1

Yadav

Tiwari

2021

Analytical Biochemistry

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Electroactive bacteria as whole-cell biosensor for sensitive detection of hydrogen peroxide

Wen

He²,

Zhuo

et al. 2023

Journal of Environmental Chemical Engineering

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A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity

Abstract: A label-free, simple and rapid colorimetric method for the sensitive detection of S1 nuclease activity based on protamine-assisted aggregation of gold nanoparticles.

Cited by 7 publications

References 63 publications

General Gel-sol Method to Synthesize Various Highly Fluorescent Nanoclusters and Assay of Nuclease with the Near Infrared-emitting Gold Nanoclusters

General Gel-sol Method to Synthesize Various Highly Fluorescent Nanoclusters and Assay of Nuclease with the Near Infrared-emitting Gold Nanoclusters

Polydiacetylene vesicles acting as colorimetric sensor for the detection of plantaricin LD1

Electroactive bacteria as whole-cell biosensor for sensitive detection of hydrogen peroxide

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