A label-free, impedance-based real time assay to identify drug-induced toxicities and differentiate cytostatic from cytotoxic effects

Kustermann, Stefan; Boess, Franziska; Buneß, Andreas; Schmitz, Markus; Watzele, Manfred; Weiser, Thomas; Singer, Thomas P.; Suter, Laura; Roth, Adrian

doi:10.1016/j.tiv.2012.08.019

Cited by 81 publications

(82 citation statements)

References 25 publications

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“…This technique successfully characterized the type of cellular responses of importance in our research group [19,20] as well as others [16,[21][22][23].…”

Section: Introductionmentioning

confidence: 94%

“…RT-CES graphs were used to determine whether the responses to treatment were cytotoxic or cytostatic by using guidelines published by Kustermann et al (2013) [16]. The line at CI = 1 divided the graphs into viable / proliferating cells (CI > 1) and nonproliferating / dying cells (CI < 1) from each other ( Fig.…”

Section: Real Time Cell Analysis Of Plant Extract Treated Hela Cellsmentioning

confidence: 99%

“…Cytostatic responses are usually employed to stop uncontrolled tumour growth while cytotoxic responses lead to cell death caused by apoptosis, necrosis or cell lysis [16]. Cytostatic and cytotoxic behaviour plays an important role in the progression of a drug into preclinical and clinical studies.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cellular injury evidenced by impedance technology and infrared microspectroscopy

Roux

Prinsloo

Meyer

2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…This technique successfully characterized the type of cellular responses of importance in our research group [19,20] as well as others [16,[21][22][23].…”

Section: Introductionmentioning

confidence: 94%

Section: Real Time Cell Analysis Of Plant Extract Treated Hela Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Cellular injury evidenced by impedance technology and infrared microspectroscopy

Roux

Prinsloo

Meyer

2015

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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“…However, further validation of HTS applications of impedance‐based instruments/techniques is advisable. To date, several studies using cell impedance have demonstrated its ability to discriminate cytostatic from cytotoxic effects106 and also to predict different outcomes in cell‐based functional assays 92. Various cell types have already been tested: astrocytes,107 cardiomyocytes,94 108, 109 110 cervix cells,111, 112 eye cells,94 intestinal cells,99, 113 lung cells,82, 94, 99, 100, 103 hepatocytes,82, 93, 94, 99 kidney cells,82, 99 macrophages,100 fibroblasts,58 breast,83 and neuronal cells 114…”

Section: Impedance‐based Monitoringmentioning

confidence: 99%

High throughput toxicity screening and intracellular detection of nanomaterials

Collins

Annangi

Rubio

et al. 2016

WIREs Nanomed Nanobiotechnol

113

106

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With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read‐across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter‐experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM‐cell interactions. Validation of in vitro HTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose‐ and time‐dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label‐free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance‐based monitoring, Multiplex analysis of secreted products, and genotoxicity methods—namely High throughput comet assay, High throughput in vitro micronucleus assay, and γH2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test; possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed. WIREs Nanomed Nanobiotechnol 2017, 9:e1413. doi: 10.1002/wnan.1413For further resources related to this article, please visit the WIREs website.

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“…Briefly, a pre-titrated cell concentration of 20 000 cells/well were plated into e-plates and allowed to attach for 24hours at which point the cell index (CI) was 1 0.5 (37 C, 5 CO 2 , 95% humidity). (Kustermann et al 2013) was used to determine whether the compound had significant toxicity or not (Fig.S1). …”

Section: Real-time Electronic Sensingmentioning

confidence: 99%

Gold(I) complex of 1,1′-bis(diphenylphosphino) ferrocene–quinoline conjugate: a virostatic agent against HIV-1

et al. 2016

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A label-free, impedance-based real time assay to identify drug-induced toxicities and differentiate cytostatic from cytotoxic effects

Cited by 81 publications

References 25 publications

Cellular injury evidenced by impedance technology and infrared microspectroscopy

Cellular injury evidenced by impedance technology and infrared microspectroscopy

High throughput toxicity screening and intracellular detection of nanomaterials

Gold(I) complex of 1,1′-bis(diphenylphosphino) ferrocene–quinoline conjugate: a virostatic agent against HIV-1

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