A label-free immunosensor based on graphene nanocomposites for simultaneous multiplexed electrochemical determination of tumor markers

Jia, Xinle; Liu, Zhimin; Liu, Na; Ma, Zhanfang

doi:10.1016/j.bios.2013.09.050

Cited by 123 publications

(31 citation statements)

References 29 publications

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“…[117] The base was treated with RGO nanoparticles, AuNPs, and thionine on one side and Prussian blue on the other side. The thionine side was then incubated with anti-CEA and the Prussian blue side was incubated with anti-AFP.…”

Section: 0 Protein and Biomarkersmentioning

confidence: 99%

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Yang

Denno

Pyakurel

et al. 2015

Analytica Chimica Acta

480

263

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Carbon nanomaterials are advantageous for electrochemical sensors because they increase the electroactive surface area, enhance electron transfer, and promote adsorption of molecules. Carbon nanotubes (CNTs) have been incorporated into electrochemical sensors for biomolecules and strategies have included the traditional dip coating and drop casting methods, direct growth of CNTs on electrodes and the use of CNT fibers and yarns made exclusively of CNTs. Recent research has also focused on utilizing many new types of carbon nanomaterials beyond CNTs. Forms of graphene are now increasingly popular for sensors including reduced graphene oxide, carbon nanohorns, graphene nanofoams, graphene nanorods, and graphene nanoflowers. In this review, we compare different carbon nanomaterial strategies for creating electrochemical sensors for biomolecules. Analytes covered include neurotransmitters and neurochemicals, such as dopamine, ascorbic acid, and serotonin; hydrogen peroxide; proteins, such as biomarkers; and DNA. The review also addresses enzyme-based electrodes that are used to detect non-electroactive species such as glucose, alcohols, and proteins. Finally, we analyze some of the future directions for the field, pointing out gaps in fundamental understanding of electron transfer to carbon nanomaterials and the need for more practical implementation of sensors.

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Section: 0 Protein and Biomarkersmentioning

confidence: 99%

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Yang

Denno

Pyakurel

et al. 2015

Analytica Chimica Acta

480

263

View full text Add to dashboard Cite

show abstract

“…The fascinated characteristics have stimulated people's interest to fabricate immunoassay by employing gold nanoparticles as the skeletons for carrying antibodies or antigen biomolecules. Generally speaking, gold nanoparticles can bind kinds of substances to build up efficient electrochemical transducer surfaces, which containing ionic liquid [23,24], metal nanoparticles [25], mesoporous silica [26], amino acid [27], protein [28,29], polymers [30][31][32][33], carbon nanoparticles [34][35][36], and electron mediator [37,38], et al For example, a low-cost and robust impedimetric immunoassay based on gold nanoparticles modified free-standing graphene paper electrode was developed by Ying and his workers for rapid and sensitive detection of Escherichia coli O157:H7 [39]. Results show that the developed paper immunoassay possesses greatly enhanced sensing performance, such as wide linear range (1.5×10 2 -1.5× 10 2 cfu mL −1 ), low detection limit (1.5×10 2 cfu mL −1 ), and excellent specificity.…”

Section: Noble Metal Nanoparticles As Matrices For Non-enzymatic Elecmentioning

confidence: 99%

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

Tang

2015

Microchim Acta

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Electrochemical immunodetection has attracted considerable attention due to its high sensitivity, low cost and simplicity. Large efforts have recently made in order to design ultrasensitive assays. Noble metal nanoparticles (NM-NPs) offer advantages such as high conductivity and large surface-to-volume ratio. NM-NPs therefore are excellent candidates for developing electrochemical platforms for immunodetection and as signal tags. The use of biofunctionalized NM-NPs often results in amplified recognition via stronger loading of signal tags, and also in enhanced signal. This review (with 87 references) gives an overview on the current state in the use of NM-NPs in Nonenzymatic electrochemical immunosensing. We discuss the application of NM-NPs as electrode matrices and as electroactive labels (either as a carrier or as electrocatalytic labels), and compare the materials (mainly nanoparticles of gold, platinum, or of bimetallic materials) in terms of performance (for example by increasing sensitivity via label amplification or via high densities of capture molecules). A conclusion covers current challenges and gives an outlook. Rather than being exhaustive, the review focuses on representative examples that illustrate novel concepts and promising applications. NM-NPs based immunosensing opens a series of concepts for basic research and offers new tools for determination of trace amounts of protein-related analytes in environment and clinical applications.

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“…Considerable efforts have focused on the modification of electrode materials with graphene and its derivatives, alone or [191] Thiolated DNA probe/G1 PAMAM * /AuNP/ graphene/Au electrode 1 pM-1 lM (1 pM) [192] ssDNA/azophloxine/graphene nanosheets/ GCE 1 fM-0.1 pM (0.4 fM) [193] ssDNA/Au nanorods/GO/GCE 0.1 fM-1 nM (3.5 fM) [194] ssDNA/Au nanorods/GO/GCE 0.1 fM-1 nM (0.035 fM) [195] ssDNA/AuNP/polyaniline/graphene sheetschitosan/GCE 10-1000 pM (2.11 pM) [196] ssDNA/AuNP-p-aminothiophenolfunctionalized GO/GCE 0.1 pM-0.1 lM (0.011 pM) [197] ssDNA/Chitosan-Co 3 O 4 nanorods-graphene/ carbon ionic liquid electrode 1 pM-1 lM (0.43 pM) [198] ssDNA/GO-chitosan/ITO 10 fM-50 nM (10 fM E.Coli O157:H7 Anti-E. coli O157:H7 Ab/AuNPs/graphene paper electrode 1.5 · 10 2 -1.5 · 10 7 cfu mL À1 (1.5 · 10 2 cfu mL À1 ) [203] Lysozyme Lysozyme binding aptamers/graphene/GCE 0.01-0.5 pM (6 fM) [204] a-fetoprotein (AFP) Ag-GO-Nafion/Au electrode 0.01-100 ng mL À1 (3 pg mL À1 ) [205] Anti-AFP Ab/Chitosan-TiO 2 -graphene/GCE 0.1-10 ng mL À1 10-300 ng mL À1 (0.03 ng mL À1 ) [206] Anti-AFP/AuNP/Prussian blue/reduced GO/ GCE 0.01-300 ng mL À1 (0.885 pg mL À1 ) [207] Anti-CEA Ab/AuNP/graphene/GCE 0.001-10 ng mL À1 (0.35 pg mL À1 ) [208] HRP/HRP-anti-CEA Ab/Au-graphene/GCE 0.1-80 ng mL À1 (0.04 ng mL À1 ) [209] Carcinoembryonic antigen (CEA) Anti-CEA Ab/chitosan/reduced GO/GCE 0.05 pg mL À1 -1 ng mL À1 (0.024 pg mL À1 ) [210] Anti-CEA/AuNP/thionine/reduced GO/GCE 0.01-300 ng mL À1 (0.650 pg mL À1 ) [207] Anti-PSA Ab/magnetic graphene nanosheets/ ITO electrode 0.003-50 ng mL À1 (0.72 pg mL À1 ) [211] Prostate specific antigen (PSA)…”

Section: Other Small Electroactive Analytesmentioning

confidence: 99%

Recent advances in electrochemical biosensing schemes using graphene and graphene-based nanocomposites

Vashist

Luong

2015

Carbon

210

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A label-free immunosensor based on graphene nanocomposites for simultaneous multiplexed electrochemical determination of tumor markers

Cited by 123 publications

References 29 publications

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: A review

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

Recent advances in electrochemical biosensing schemes using graphene and graphene-based nanocomposites

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