A label-free electrochemical impedance immunosensor based on AuNPs/PAMAM-MWCNT-Chi nanocomposite modified glassy carbon electrode for detection of Salmonella typhimurium in milk

Dong, Jing; Zhao, Han; Xu, Minrong; Ma, Qiang; Ai, Shiyun

doi:10.1016/j.foodchem.2013.04.098

Cited by 146 publications

(65 citation statements)

References 29 publications

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“…Dong et al (2013) discussed a label-free impedance immunosensor for the detection of Salmonella Typhimurium developed by immobilizing Salmonella antibodies on gold nanoparticles and poly (amidoamine)-multi-walled carbon nanotubes -chitosan nanocomposite film modified glassy carbon electrode. This biosensor, able to measure concentrations from 10 3 to 10 7 CFU mL −1 in pure culture, was successfully tested also with real milk samples.…”

Section: Impedance Biosensorsmentioning

confidence: 99%

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Grossi

Riccò

2017

J. Sens. Sens. Syst.

296

160

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Abstract. Electrical impedance spectroscopy (EIS), in which a sinusoidal test voltage or current is applied to the sample under test to measure its impedance over a suitable frequency range, is a powerful technique to investigate the electrical properties of a large variety of materials. In practice, the measured impedance spectra, usually fitted with an equivalent electrical model, represent an electrical fingerprint of the sample providing an insight into its properties and behavior. EIS is used in a broad range of applications as a quick and easily automated technique to characterize solid, liquid, semiliquid, organic as well as inorganic materials. This paper presents an updated review of EIS main implementations and applications.

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Section: Impedance Biosensorsmentioning

confidence: 99%

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Grossi

Riccò

2017

J. Sens. Sens. Syst.

296

160

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“…The fascinated characteristics have stimulated people's interest to fabricate immunoassay by employing gold nanoparticles as the skeletons for carrying antibodies or antigen biomolecules. Generally speaking, gold nanoparticles can bind kinds of substances to build up efficient electrochemical transducer surfaces, which containing ionic liquid [23,24], metal nanoparticles [25], mesoporous silica [26], amino acid [27], protein [28,29], polymers [30][31][32][33], carbon nanoparticles [34][35][36], and electron mediator [37,38], et al For example, a low-cost and robust impedimetric immunoassay based on gold nanoparticles modified free-standing graphene paper electrode was developed by Ying and his workers for rapid and sensitive detection of Escherichia coli O157:H7 [39]. Results show that the developed paper immunoassay possesses greatly enhanced sensing performance, such as wide linear range (1.5×10 2 -1.5× 10 2 cfu mL −1 ), low detection limit (1.5×10 2 cfu mL −1 ), and excellent specificity.…”

Section: Noble Metal Nanoparticles As Matrices For Non-enzymatic Elecmentioning

confidence: 99%

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

Tang

2015

Microchim Acta

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Electrochemical immunodetection has attracted considerable attention due to its high sensitivity, low cost and simplicity. Large efforts have recently made in order to design ultrasensitive assays. Noble metal nanoparticles (NM-NPs) offer advantages such as high conductivity and large surface-to-volume ratio. NM-NPs therefore are excellent candidates for developing electrochemical platforms for immunodetection and as signal tags. The use of biofunctionalized NM-NPs often results in amplified recognition via stronger loading of signal tags, and also in enhanced signal. This review (with 87 references) gives an overview on the current state in the use of NM-NPs in Nonenzymatic electrochemical immunosensing. We discuss the application of NM-NPs as electrode matrices and as electroactive labels (either as a carrier or as electrocatalytic labels), and compare the materials (mainly nanoparticles of gold, platinum, or of bimetallic materials) in terms of performance (for example by increasing sensitivity via label amplification or via high densities of capture molecules). A conclusion covers current challenges and gives an outlook. Rather than being exhaustive, the review focuses on representative examples that illustrate novel concepts and promising applications. NM-NPs based immunosensing opens a series of concepts for basic research and offers new tools for determination of trace amounts of protein-related analytes in environment and clinical applications.

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“…The binding of Salmonella cells to the specific antibodies attached to the modified electrode increased the electron transfer resistance measured by EIS using [Fe(CN) 6 ] 3−/4− as the redox probe. A linear relationship was obtained between 1.0 × 10 3 to 1.0 × 10 7 cfu·mL −1 with a detection limit of 5.0 × 10 2 cfu·mL −1 .The method was successfully applied to determine S. typhimurium content in milk samples [122]. A label-free impedimetric aptamer-based biosensor for S. typhimurium was also fabricated by grafting a diazonium-supporting layer onto SPCEs followed by immobilization of an aminated-aptamer.…”

Section: Electrochemical Affinity Biosensors For Microorganismsmentioning

confidence: 98%

“…), which is of key importance in food and water quality monitoring. Table 4 summarizes relevant information regarding recent reports on electrochemical affinity biosensors for microorganisms [118][119][120][121][122][123][124][125][126] and some important features of selected configurations are commented below.…”

Section: Electrochemical Affinity Biosensors For Microorganismsmentioning

confidence: 99%

Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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A label-free electrochemical impedance immunosensor based on AuNPs/PAMAM-MWCNT-Chi nanocomposite modified glassy carbon electrode for detection of Salmonella typhimurium in milk

Cited by 146 publications

References 29 publications

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

Electrochemical Affinity Biosensors in Food Safety

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