A label-free electrochemical immunoassay for IgG detection based on the electron transfer

Qiu, Liping; Wang, Cunchang; Hu, Peng; Wu, Zai‐Sheng; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1016/j.talanta.2010.08.036

Cited by 32 publications

(15 citation statements)

References 25 publications

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“…Electrochemical approaches are useful for detecting protein samples as they have the important advantage of offering label-free and quantitative measurement capability [40–42]. The majority of optical detection methodologies is faced with the significant challenge of inferring accurate and quantitative results from the collected optical signals during measurements.…”

Section: Existing Methods For Analytical Detection Of Proteinsmentioning

confidence: 99%

Functional Polymers in Protein Detection Platforms: Optical, Electrochemical, Electrical, Mass-Sensitive, and Magnetic Biosensors

Hahm

2011

Sensors

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The rapidly growing field of proteomics and related applied sectors in the life sciences demands convenient methodologies for detecting and measuring the levels of specific proteins as well as for screening and analyzing for interacting protein systems. Materials utilized for such protein detection and measurement platforms should meet particular specifications which include ease-of-mass manufacture, biological stability, chemical functionality, cost effectiveness, and portability. Polymers can satisfy many of these requirements and are often considered as choice materials in various biological detection platforms. Therefore, tremendous research efforts have been made for developing new polymers both in macroscopic and nanoscopic length scales as well as applying existing polymeric materials for protein measurements. In this review article, both conventional and alternative techniques for protein detection are overviewed while focusing on the use of various polymeric materials in different protein sensing technologies. Among many available detection mechanisms, most common approaches such as optical, electrochemical, electrical, mass-sensitive, and magnetic methods are comprehensively discussed in this article. Desired properties of polymers exploited for each type of protein detection approach are summarized. Current challenges associated with the application of polymeric materials are examined in each protein detection category. Difficulties facing both quantitative and qualitative protein measurements are also identified. The latest efforts on the development and evaluation of nanoscale polymeric systems for improved protein detection are also discussed from the standpoint of quantitative and qualitative measurements. Finally, future research directions towards further advancements in the field are considered.

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Section: Existing Methods For Analytical Detection Of Proteinsmentioning

confidence: 99%

Functional Polymers in Protein Detection Platforms: Optical, Electrochemical, Electrical, Mass-Sensitive, and Magnetic Biosensors

Hahm

2011

Sensors

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“…A comparison of the proposed method with other labelfree immunoassays for the determination of hIgG was made (Table 1) [1, [28][29][30][31][32][33]. The results show that the limit of detection of the present method is superior to most previously reported methods for the determination of hIgG.…”

Section: Analytical Performance Of the Label-free Ecl Immunosensormentioning

confidence: 86%

Ultrasensitive label-free electrochemiluminescence immunosensor based on N-(4-aminobutyl)-N-ethylisoluminol-functionalized graphene composite

2015

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The electrochemiluminescence (ECL) behavior of N-(4-aminobutyl)-N-ethylisoluminol (ABEI)-functionalized graphene composite (ABEI-GC) modified on an indium tin oxide (ITO) electrode was studied. ABEI-GC exhibited excellent ECL activity. On this basis, a label-free ECL immunosensor was developed for the sensitive detection of human immunoglobulin G (hIgG) by using ABEI-GC as the ECL nano-interface via a layer-by-layer assembly technique. ABEI-GC was first assembled onto an ITO electrode. Positively charged chitosan was then electrostatically adsorbed to the modified electrode. Finally, negatively charged antibody-coated gold nanoparticles were attached to the surface to form the ECL immunosensor. In the presence of hIgG, hIgG was captured by its antibody. In addition, an ECL signal was detected in the presence of H 2 O 2 when a double potential was applied. The ECL immunosensor for the determination of hIgG showed a linear range of 1.0×10 13 -1.0×10 8 g/mL with a detection limit of 5.0×10 14 g/mL. This immunosensor has high sensitivity, wide linearity and good reproducibility. The superior sensitivity of the proposed ECL immunoassay mainly derives from the incorporation of ABEI-GC, which not only improves the ECL intensity, response speed, and stability, but also provides a large specific surface for high levels of protein loading. This work reveals that ABEI-GC is good nano-interface for the construction of ECL biosensors. Our strategy is promising for protein detection and may open up a new avenue for ultrasensitive label-free immunoassays. N-(aminobutyl)-N-(ethylisoluminol) (ABEI), graphene, electrochemiluminescence immunosensor

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“…This monitoring method is very attractive because it is simple and does not require a complicated labeling process. (10,11) The label-free immunosensor detects the target on the basis of a change (amplitude, conductive, piezoelectricity, etc.) due to the binding of the target compound to the immobilized antibody on the sensor bioreceptor region.…”

Section: Introductionmentioning

confidence: 99%

Carbon-Nanotube-Enhanced Label-Free Immunosensor for Highly Sensitive Detection of Plasma Cortisol Level in Fish

2015

Sensors and Materials

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Fish display a wide variation in their physiological responses to stress, which is clearly evident in plasma corticosteroid changes, chiefly the cortisol level, in fish. Cortisol is a well-known indicator of fish stress; thus, a simple and speedy approach to detecting plasma cortisol should be developed. Here we described a novel disposable carbon-nanotube-enhanced label-free immunosensor for detecting plasma cortisol level. The principle of the sensor system is based on differences in the electrochemical change in oxidation peak current induced by an immunoreaction that depends on the cortisol level of the sample. We used a single-walled carbon nanotube to improve the sensitivity and conductivity of the system. The proposed sensor system showed a linear correlation (R = 0.992) between cortisol level and oxidation peak current in the range from 156 to 10000 pg ml −1 . The specificity of the label-free immunosensor system was investigated using other steroid hormones; the specificity of cortisol was suggested by a minimal change in the oxidation peak current of the other steroid hormones. The sensor system was used to determine the plasma cortisol level of Nile tilapia (Oreochromis niloticus) and the results were compared with those of the same samples determined using a conventional method (ELISA). A very good correlation was obtained between results determined using both methods (correlation coefficient, 0.999).

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A label-free electrochemical immunoassay for IgG detection based on the electron transfer

Cited by 32 publications

References 25 publications

Functional Polymers in Protein Detection Platforms: Optical, Electrochemical, Electrical, Mass-Sensitive, and Magnetic Biosensors

Functional Polymers in Protein Detection Platforms: Optical, Electrochemical, Electrical, Mass-Sensitive, and Magnetic Biosensors

Ultrasensitive label-free electrochemiluminescence immunosensor based on N-(4-aminobutyl)-N-ethylisoluminol-functionalized graphene composite

Carbon-Nanotube-Enhanced Label-Free Immunosensor for Highly Sensitive Detection of Plasma Cortisol Level in Fish

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