A label-free electrochemical aptasensor for the detection of kanamycin in milk

Zhou, Nandi; Luo, Jibao; Zhang, Juan; You, Yuanding; Tian, Yaping

doi:10.1039/c4ay02710h

Cited by 47 publications

(17 citation statements)

References 31 publications

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“…The electron transfer resistance increased with increasing concentration of DEHP. This result is consistent with a previous report [35], indicating an increase in electron transfer resistance with conformational change of aptamer.…”

Section: Fabrication Of Electrochemical Impedance Spectroscope Aptasesupporting

confidence: 94%

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Selection of Aptamers Specific for DEHP Based on ssDNA Library Immobilized SELEX and Development of Electrochemical Impedance Spectroscopy Aptasensor

et al. 2020

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A selection of aptamers specific for di(2-ethylhexyl) phthalate (DEHP) and development of electrochemical impedance spectroscopy (EIS) aptasensor are described in this paper. The aptamers were selected from an immobilized ssDNA library using the systematic evolution of ligands by exponential enrichment (SELEX). The enrichment was monitored using real-time quantitative PCR (Q-PCR), and the aptamers were identified by high-throughput sequencing (HTS), gold nanoparticles (AuNPs) colorimetric assay, and localized surface plasmon resonance (LSPR). The EIS aptasensor was developed to detect DEHP in water samples. After eight rounds of enrichment, HTS, AuNPs colorimetric assay, and LSPR analysis indicated that four aptamers had higher binding activity, and aptamer 31 had the highest affinity (Kd = 2.26 ± 0.06 nM). The EIS aptasensor had a limit of detection (LOD) of 0.103 pg/mL with no cross-reactivity to DEHP analogs and a mean recovery of 76.07% to 141.32% for detection of DEHP in water samples. This aptamer is novel with the highest affinity and sensitivity.

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Section: Fabrication Of Electrochemical Impedance Spectroscope Aptasesupporting

confidence: 94%

“…previous report [35], indicating an increase in electron transfer resistance with conformational change of aptamer.…”

Section: Fabrication Of Electrochemical Impedance Spectroscope Aptasementioning

confidence: 49%

Selection of Aptamers Specific for DEHP Based on ssDNA Library Immobilized SELEX and Development of Electrochemical Impedance Spectroscopy Aptasensor

et al. 2020

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“…In the past decades, various methods have been reported for the detection of Kana residues such as microbiological agar diffusion (M-agar) assay (Maitra et al 1979), gas chromatography (GC) (Isoherranen and Soback 1999), and capillary electrophoresis (CE) (Long et al 2003), high-performance liquid chromatography (HPLC) (Blanchaert et al 2013), electrochemical immunosensor (Wei et al 2012), electrochemical aptasensor (Zhou et al 2015). These are often complex and require tedious sample pretreatment steps.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Artificial Antigen and Development of IgY-Based Indirect Competitive ELISA for the Detection of Kanamycin Residues

2015

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The present study was aimed to evaluate the feasibility to use chicken egg yolk antibodies (IgY) for the detection of Kanamycin (Kana) residues in food stuffs. Bovine serum albumin (BSA) was conjugated with Kana by carbodiimide method, and the laying chickens were immunized with Kana-BSA conjugate. PEG-6000 method was employed to extract IgY. The peak titer of anti-Kana IgY was 1:256,000 after the fifth booster immunization. The optimal dilution for anti-Kana IgY was 1:25,000 to obtain optical density (OD) of 1.0 at 2 μg/mL of OVA-Kana coating concentration. The indirect competitive ELISA (ic-ELISA) showed that the IC 50 value of anti-Kana IgY was 4.48 ng/ mL and the regression curve equation was y =−15.62x+ 60.17 (R 2 =0.98, n=3). The ic-ELISA showed a lower crossreactivity (less than 0.01 %) with other drugs (except gentamicin-1.91 %). Recoveries from milk, pork, and chicken powder samples were in the range of 82.02 to 98.20 %, with relative standard deviation lower than 5.13 %. Our results indicate that generated anti-Kana IgY can be used in routine screening analysis of Kana residues in food samples.

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“…8,9 Consequently, aptamers are gaining popularity in the eld of biosensing. 10 Since the discovery of the kanamycin binding aptamer (KBA), 11 there have been several attempts to develop KBA based sensors for the detection of residual kanamycin using different optical [12][13][14][15][16][17][18][19][20][21] and electrochemical [22][23][24][25][26][27][28][29][30][31][32][33] schemes. Out of these, uorescent aptasensors have several advantages due to their simpler implementation schemes and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Smartphone-based kanamycin sensing with ratiometric FRET

et al. 2019

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A label-free electrochemical aptasensor for the detection of kanamycin in milk

Abstract: Kanamycin is detected based on the conformational change of the aptamer attached to the electrode surface and the corresponding SWV current change in [Fe(CN)6]3−/4− solution.

Cited by 47 publications

References 31 publications

Selection of Aptamers Specific for DEHP Based on ssDNA Library Immobilized SELEX and Development of Electrochemical Impedance Spectroscopy Aptasensor

Selection of Aptamers Specific for DEHP Based on ssDNA Library Immobilized SELEX and Development of Electrochemical Impedance Spectroscopy Aptasensor

Preparation of Artificial Antigen and Development of IgY-Based Indirect Competitive ELISA for the Detection of Kanamycin Residues

Smartphone-based kanamycin sensing with ratiometric FRET

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