A Label-Free Colorimetric Aptasensor Containing DNA Triplex Molecular Switch and AuNP Nanozyme for Highly Sensitive Detection of Saxitoxin

When nanozymes meet deoxyribonucleic acid: Understanding their interactions and biomedical diagnosis applications

“…51 Currently, in many studies, one has confirmed that the adsorption of ssDNA can promote the enzyme-like activity of various materials. [55][56][57][58][59][69][70][71][72][73][74][75][76][77][78][79][80][81][82][83][84][85] The activity enhancement is mainly attributed to the increased surface negativity of nanozymes after the adsorption of DNA, which can enhance the diffusion and adsorption of positively charged substrates like TMB, thus resulting in the enrichment of the substrates toward the nanozyme surface and the improvement of catalytic efficiency. Liu's group demonstrated that Fe 3 O 4 NPs showed certain POD-like activity.…”

“…Identifying these risks and taking corresponding preventive measures is a major demand for protecting human health. Currently, the interactions between DNA chains (aptamers) and nanozymes have been applied to establish biosensors for the monitoring of various health risk factors, including antibiotics, 59,62,65,76,84,91 toxins, 56,69,80,100,103,131,132 pesticide residues, 90,97 and pathogens and viruses. 51,63,66,[93][94][95]133 The emergence and application of antibiotics is a major revolution in the history of human medicine.…”

“…Currently, some studies have realized the monitoring of biotoxins using the DNA or aptamer-mediated nanozyme activity modulation principle. 56,69,80,100,103,131,132 Typically, Li et al designed a terminal-fixed anti-saxitoxin (STX) aptamer with a loop recognition structure, 56 and the extended two-terminal sequences could hybridize to form a stem-loop structure. It was found that the terminal-fixed aptamer could significantly increase its affinity to the target.…”

“…Food poisoning caused by biotoxins often occurs, which seriously threatens human health. Currently, some studies have realized the monitoring of biotoxins using the DNA or aptamer‐mediated nanozyme activity modulation principle 56,69,80,100,103,131,132 . Typically, Li et al.…”

Detection of saxitoxin by a SERS aptamer sensor based on enzyme cycle amplification technology

“…The receptors and antibodies used in RBA and ELISA are easily affected by the environment and have poor stability, requiring strict storage conditions. In addition, the colorimetric method, [14][15][16][17] fluorescence method, 18,19 and electrochemical detection method [20][21][22][23][24] are also used to detect the toxins of the clam. The measurable range and stability of the colorimetric method are better than those of other rapid detection methods, but the sensitivity is limited.…”