A label-free colorimetric aptasensor based on split aptamers-chitosan oligosaccharide-AuNPs nanocomposites for sensitive and selective detection of kanamycin

Qi, Xiaoyan; Zhao, Yinglin; Su, Haipeng; Wang, Lele; Li, Ling; Ma, Rui; Yan, Xiaochen; Sun, Jianan; Wang, Qianqian; Mao, Xiangzhao

doi:10.1016/j.talanta.2021.123032

Cited by 32 publications

(11 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AuNPs-based aptasensors have been widely applied in the detection of various targets including chemical compounds, proteins, pesticides, toxins, and viruses [ 36 , 38 , 39 , 40 , 48 , 49 , 50 ]. Their simple preparation, rapid detection, and the fact that they do not require special instrumentation have made them the preferred choice for PoCT [ 51 ].…”

Section: Resultsmentioning

confidence: 99%

“…If the analyte for the specific MRE is present, the MRE binds to the analyte instead of the AuNPs and the colour of the AuNPs will change from ruby red to purple/blue. Based on this principle, several AuNP-based optical aptasensors have been developed [ 36 , 37 , 38 , 39 , 40 ]. In this study, using this principle, a simple yet effective, specific, and rapid RBP-A-aptasensor for the detection of RBP4 was developed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Label-Free Gold Nanoparticles-Based Optical Aptasensor for the Detection of Retinol Binding Protein 4

et al. 2022

View full text Add to dashboard Cite

Retinol-binding protein 4 (RBP4) has been implicated in insulin resistance in rodents and humans with obesity and T2DM, making it a potential biomarker for the early diagnosis of T2DM. However, diagnostic tools for low-level detection of RBP4 are still lagging behind. Therefore, there is an urgent need for the development of T2DM diagnostics that are rapid, cost-effective and that can be used at the point-of-care (POC). Recently, nano-enabled biosensors integrating highly selective optical detection techniques and specificity of aptamers have been widely developed for the rapid detection of various targets. This study reports on the development of a rapid gold nanoparticles (AuNPs)-based aptasensor for the detection of RBP4. The retinol-binding protein aptamer (RBP-A) is adsorbed on the surface of the AuNPs through van der Waals and hydrophobic interactions, stabilizing the AuNPs against sodium chloride (NaCl)-induced aggregation. Upon the addition of RBP4, the RBP-A binds to RBP4 and detaches from the surface of the AuNPs, leaving the AuNPs unprotected. Addition of NaCl causes aggregation of AuNPs, leading to a visible colour change of the AuNPs solution from ruby red to purple/blue. The test result was available within 5 min and the assay had a limit of detection of 90.76 ± 2.81 nM. This study demonstrates the successful development of a simple yet effective, specific, and colorimetric rapid assay for RBP4 detection.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Label-Free Gold Nanoparticles-Based Optical Aptasensor for the Detection of Retinol Binding Protein 4

et al. 2022

View full text Add to dashboard Cite

show abstract

“…These are capable of binding specific targets with high affinity, specificity, and sensitivity [ 26 , 27 ]. The target molecule can be as simple as a metal ion, and can be as complex as large protein molecules such as cardiac troponin, or even cells [ 28 , 29 , 30 , 31 , 32 ]. Aptamers are designed by an easier, cheaper in vitro method called SELEX (systematic evolution of ligands by exponential enrichment) [ 33 , 34 ].…”

Section: Label and Label-free Aptasensorsmentioning

confidence: 99%

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

et al. 2022

View full text Add to dashboard Cite

Viral infections are becoming the foremost driver of morbidity, mortality and economic loss all around the world. Treatment for diseases associated to some deadly viruses are challenging tasks, due to lack of infrastructure, finance and availability of rapid, accurate and easy-to-use detection methods or devices. The emergence of biosensors has proven to be a success in the field of diagnosis to overcome the challenges associated with traditional methods. Furthermore, the incorporation of aptamers as bio-recognition elements in the design of biosensors has paved a way towards rapid, cost-effective, and specific detection devices which are insensitive to changes in the environment. In the last decade, aptamers have emerged to be suitable and efficient biorecognition elements for the detection of different kinds of analytes, such as metal ions, small and macro molecules, and even cells. The signal generation in the detection process depends on different parameters; one such parameter is whether the labelled molecule is incorporated or not for monitoring the sensing process. Based on the labelling, biosensors are classified as label or label-free; both have their significant advantages and disadvantages. Here, we have primarily reviewed the advantages for using aptamers in the transduction system of sensing devices. Furthermore, the labelled and label-free opto-electrochemical aptasensors for the detection of various kinds of viruses have been discussed. Moreover, numerous globally developed aptasensors for the sensing of different types of viruses have been illustrated and explained in tabulated form.

show abstract

“…Molecular docking could simulate aptamer–ligand interactions and completely characterize complexes, 23 allowing for generating accurate three-dimensional (3D) models and the elucidation of detailed binding sites. 24 Ma et al 25 used molecular docking to predict the critical bases of aptamer binding to chloramphenicol, and then trimmed the 80-base aptamer to 30 bases for designing the biosensor. Through molecular docking, the recognition mechanism between the aptamer and target can be analyzed and quantified in detail to construct a highly sensitive and reliable apasensor, which involved a complementary sequence and competitive strategy.…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive competitive aptasensor for AFB₁ detection based on an exonuclease-assisted target recycling amplification strategy

Zhan

Yang

et al. 2023

Anal. Methods

View full text Add to dashboard Cite

show abstract

A label-free colorimetric aptasensor based on split aptamers-chitosan oligosaccharide-AuNPs nanocomposites for sensitive and selective detection of kanamycin

Cited by 32 publications

References 35 publications

A Label-Free Gold Nanoparticles-Based Optical Aptasensor for the Detection of Retinol Binding Protein 4

A Label-Free Gold Nanoparticles-Based Optical Aptasensor for the Detection of Retinol Binding Protein 4

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

A highly sensitive competitive aptasensor for AFB₁ detection based on an exonuclease-assisted target recycling amplification strategy

Contact Info

Product

Resources

About

A label-free colorimetric aptasensor based on split aptamers-chitosan oligosaccharide-AuNPs nanocomposites for sensitive and selective detection of kanamycin

Cited by 32 publications

References 35 publications

A Label-Free Gold Nanoparticles-Based Optical Aptasensor for the Detection of Retinol Binding Protein 4

A Label-Free Gold Nanoparticles-Based Optical Aptasensor for the Detection of Retinol Binding Protein 4

Ultrasensitive Aptasensors for the Detection of Viruses Based on Opto-Electrochemical Readout Systems

A highly sensitive competitive aptasensor for AFB1 detection based on an exonuclease-assisted target recycling amplification strategy

Contact Info

Product

Resources

About

A highly sensitive competitive aptasensor for AFB₁ detection based on an exonuclease-assisted target recycling amplification strategy