A label-free and enzyme-free fluorescent aptasensor for sensitive detection of acetamiprid based on AT-rich dsDNA-templated copper nanoparticles

Copper nanoclusters: designed synthesis, structural diversity, and multiplatform applications

“…The sensor could operate in a wide analytical range of 1-40 μM with LOD of 0.25 μM. Kaimei Fan et al298 and Yue Yang et al299 applied CuNCs for the detection of neonicotinoid insecticides such as acetamiprid (brand name: Assail) and dinotefuran in water and food samples. The lowest detectable amounts of acetamiprid and dinotefuran were 2.37 nM 298 and 7.04 μM 299 using dsDNA-and GSH-CuNCs-based fluorometric probes, respectively.…”

DNA‐Templated Copper Nanoprobes: Overview, Feature, Application, and Current Development in Detection Technologies

“…Besides, acetamiprid was used for the target by aptasensor detection, which was sensing by Cu-NPs with AT-rich dsDNA structure. [41] This method was exhibiting a good linear range from 5 to 500 nM, and detection limit of 2.37 nM. It noted that the detection platform of acetamiprid in diluted vegetable and fruit extracts was also successfully achieved.…”

“…This method obtained a linear range from 0.01 to 100 nM, and the detection limit of 5 pM. Besides, acetamiprid was used for the target by aptasensor detection, which was sensing by Cu‐NPs with AT‐rich dsDNA structure . This method was exhibiting a good linear range from 5 to 500 nM, and detection limit of 2.37 nM.…”

Copper and copper nanoparticles toxicity and their impact on basic functions in the body

“…Advanced production techniques like thermal sintering (147), laser assisted sintering (148), electrodeposition (149), biotechnological processes (150), standard chemical synthesis (151,152) and click chemistry (151) and chemical properties. Antimicrobial applications (153,154) or other regulation of microorganism growth (155), use of unique optical properties like fl uorescence (156) including application to improving of quantum dots properties (157) and sensor systems, where the copper nanoparticles can carry another functional part like biomolecules (158) or serve as pseudoenzymes (159)(160)(161) are examples of specifi c use. When the nanoparticles appeared, toxicity of them became recognized as an important issue because they can cause pollution of environment and occupational risks.…”