A lab-on-a-chip device for investigating the fusion process of olfactory ensheathing cell spheroids

Abstract

Understanding the process of fusion of olfactory ensheathing cell spheroids will lead to improvement of cell transplantation therapies to repair spinal cord injuries. The successful fusion of transplanted spheroids will enable alternative transplantation strategies to be developed for in vivo applications. This paper describes the use of a microfluidic device to trap and fuse olfactory ensheathing cell spheroids. The velocity, the pressure distribution in the device were simulated numerically to predict the trapping location. The simulation predicted the optimum flow rates for trapping the spheroids in the later experiments. Simulated particle trajectories were verified experimentally with tracing of fluorescent micro particles. The fusion process of the spheroids was investigated over a period of 48 hours. The microfluidic platform presented here can be used for testing potential drugs that can promote the fusion process and improve the transplantation therapy.

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

“…Although there are numerous µSFCs, they can be divided into two principal categories: 1) those using emulsion-based techniques, or; 2) those utilizing microwells or U-shaped microstructures. Some other devices under investigation are culture-only chips, meaning that spheroid formation must first proceed using different methods outside the chip, before introduction to the chip for culture and drug efficacy testing [6,8,87]. We refer to these latter devices as microfluidic spheroid culture chips (µSCCs).…”

Inventions and Innovations in Preclinical Platforms for Cancer Research

“…They have been designed with various purposes including shear stress analysis [117], drug screening [118], multi organ-on-a-chip [119] and analysis of the spheroid fusion process [120]. Digital microfluidic platforms also are used for spheroid formation and culture [121].…”

Electrocontrolled Liquid Marbles for Rapid Miniaturized Organic Reactions

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