A Klebsiella pneumoniae producing three kinds of class A β-lactamases encoded by one single plasmid isolated from a patient in Huashan Hospital, Shanghai, China

Prevalence of plasmid-mediated quinolone-resistance determinants in Shigella flexneri isolates from Anhui Province, China

“…Amplification of bla genes (b-lactamase genes) was also performed with the methods described previously for bla TEM , bla SHV and bla CTXÀM . [13][14][15] The mutations in the quinolone resistance-determining regions of the gyrA, gyrB, parC and parE genes were amplified by PCR. 16 All purified PCR products were directly sequenced using an ABI3100 genetic analyzer and continued using primers walking on both DNA strands.…”

“…CTX-M-ESBLs (especially CTX-M-14) prefer hydrolyzing cefotaxime than ceftazidime and are the main type of ESBLs in Enterobacteriaceae isolates, including Shigella spp., in China. 3,13,14 Results of PFGE show clonal spread in a few of these strains, which poses a serious threat to clinic. Some isolates (05ShC15/ 05ShC42 and 05FuY2/05FuY17) carry the same resistance genes, but the strains are in different PFGE types.…”

Prevalence of IncI1-Iγ and IncFIA-FIB type plasmids in extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains isolated from the NICU of a North Indian hospital

“…Furthermore, they are inhibited by b-lactamase inhibitors such as clavulanic acid and tazobactam. Bacteria having ESBLs marker on a plasmid are of serious concern because of cross-species dissemination of these markers (Haque et al, 2012;Xiong et al, 2004 Clinical conditions and treatments predisposing to infection by such pathogens have been investigated mostly due to the prevalence of ESBL-producing K. pneumoniae in NICUs and there is a paucity of ESBL data on neonates, particularly in North India. In view of the present situation, we initiated our study to understand the mechanism of resistance among the K. pneumoniae isolates circulating in the NICU of a teaching hospital in North India.…”

Distribución de genes codificadores de β-lactamasas de espectro extendido en aislamientos de Klebsiella pneumoniae de hospitales de Bogotá, D.C., Colombia

“…Genes codificadores de BLEE en Klebsiella pneumoniae hospitalaria de beta-lactámicos como estrategia adicional de resistencia, lo que les permite cubrir un rango amplio de antibióticos, desde las penicilinas y cefalosporinas de primera y segunda generación (sustrato de las beta-lactamasas de espectro ampliado), hasta las cefalosporinas de tercera y cuarta generación y monobactámicos (sustrato de las beta-lactamasas de espectro extendido) (28,29). Así, los diferentes genes encontrados podrían explican la gran frecuencia de microorganismos resistentes a las cefalosporinas evaluadas.…”

“…Así, los diferentes genes encontrados podrían explican la gran frecuencia de microorganismos resistentes a las cefalosporinas evaluadas. A lo anterior se le suman los porcentajes de resistencia a ciprofloxacina, gentamicina, amikacina y trimetoprimsulfametoxazol que evidencian el potencial de las bacterias estudiadas para adquirir y expresar resistencia hacia otros grupos de antibióticos y la cada vez más limitada disposición de antibióticos eficaces para tratar las infecciones causadas por estos microorganismos (29).…”