A kinetic study of microencapsulated bovine carbonic anhydrase

Boguslaski, Robert C.; Janik, Alicja

doi:10.1016/0005-2744(71)90143-4

Cited by 19 publications

(4 citation statements)

References 11 publications

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“…The catalytic efficiency ( k cat / K m ) of the soluble and immobilized BCA for p ‐NPA hydrolysis was found to be 874 and 783 M −1 s −1 , respectively. Upon comparison with free CA, the catalytic activity of FeCA was found to be 89.5 %, which is higher than that of previously reported CA encapsulated microcapsules (10 %)16a and nanogels (70 %) 16b. The CO 2 conversion efficiency was quantified by using indirect approaches.…”

Section: Resultsmentioning

confidence: 70%

Carbonic Anhydrase Immobilized on Encapsulated Magnetic Nanoparticles for CO₂ Sequestration

Vinoba

Bhagiyalakshmi

Jeong

et al. 2012

Chemistry A European J

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Bovine carbonic anhydrase (BCA) was covalently immobilized onto OAPS (octa(aminophenyl)silsesquioxane)-functionalized Fe(3)O(4)/SiO(2) nanoparticles by using glutaraldehyde as a spacer. The Fe(3)O(4) nanoparticles were coated with SiO(2), onto which was grafted OAPS, and the product was characterized using SEM, TEM, XRD, IR, X-ray photoelectron spectroscopy (XPS), and magnetometer analysis. The enzymatic activities of the free and Fe(3)O(4)/SiO(2)/OAPS-conjugated BCA (Fe-CA) were investigated by hydrolyzing p-nitrophenylacetate (p-NPA), and hydration and sequestration of CO(2) to CaCO(3). The CO(2) conversion efficiency and reusability of the Fe-CA were studied before and after washing the recovered Fe-CA by applying a magnetic field and quantifying the unreacted Ca(2+) ions by using ion chromatography. After 30 cycles, the Fe-CA displayed strong activity, and the CO(2) capture efficiency was 26-fold higher than that of the free enzyme. Storage stability studies suggested that Fe-CA retained nearly 82 % of its activity after 30 days. Nucleation of the precipitated CaCO(3) was monitored by using polarized light microscopy, which revealed the formation of two phases, calcite and valerite, at pH 10 upon addition of serine. The magnetic nanobiocatalyst was shown to be an excellent reusable catalyst for the sequestration of CO(2).

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Section: Resultsmentioning

confidence: 70%

Carbonic Anhydrase Immobilized on Encapsulated Magnetic Nanoparticles for CO₂ Sequestration

Vinoba

Bhagiyalakshmi

Jeong

et al. 2012

Chemistry A European J

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“…They can provide up to 10 -fold rate enhancement over non-catalyzed reactions (l3), Oa a weight basis, enzymes are often 100-1000 times more active than their inorganic counterparts (13). Carbonic anhydrase, which catalyzes the hydration of carbon dioxide to carbonic acid (14), is 5000 times more active than arsenite, the normal industrial catalyst for the reaction (l5). For a cornorehensive background on enzymes, their activities and specificities, a number of texts and reviews are available (12,(16)(17)(18)(19)(20).…”

Section: Transferases -Catalyze Group Transfer Reactionsmentioning

confidence: 99%

Pilot plant production of glucose with glucoamylase immobilized to porous silica

Lee

Reilly

et al. 1976

Biotech & Bioengineering

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INFORMATION TO USERSThis materia! was produced from a microfilm copy of the original document. While the most advanced technological means to photograph and reproduce this document have been used, the quality is heavily dependent upon the quality of the original submitted.The following explanation of techniques is provided to help you understand markings or patterns which may appear on this reproduction.

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“…Carbonic anhydrase is the only enzyme with the aid of bov/ine serum albumin uhich exhibited greater Hichaelis (K'm) value, i.e., 3 to 4 times greater (Boguslaski and Janik, 1971).…”

mentioning

confidence: 99%

Evaluation of dithiobis(thioformate) and S-methyl dithiocarbonate as reactive groups for the immobilization of biological molecules

Borlaza

Cheetham

Southwell-Keely

1979

Carbohydrate Research

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A kinetic study of microencapsulated bovine carbonic anhydrase

Cited by 19 publications

References 11 publications

Carbonic Anhydrase Immobilized on Encapsulated Magnetic Nanoparticles for CO₂ Sequestration

Carbonic Anhydrase Immobilized on Encapsulated Magnetic Nanoparticles for CO₂ Sequestration

Pilot plant production of glucose with glucoamylase immobilized to porous silica

Evaluation of dithiobis(thioformate) and S-methyl dithiocarbonate as reactive groups for the immobilization of biological molecules

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A kinetic study of microencapsulated bovine carbonic anhydrase

Cited by 19 publications

References 11 publications

Carbonic Anhydrase Immobilized on Encapsulated Magnetic Nanoparticles for CO2 Sequestration

Carbonic Anhydrase Immobilized on Encapsulated Magnetic Nanoparticles for CO2 Sequestration

Pilot plant production of glucose with glucoamylase immobilized to porous silica

Evaluation of dithiobis(thioformate) and S-methyl dithiocarbonate as reactive groups for the immobilization of biological molecules

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Product

Resources

About

Carbonic Anhydrase Immobilized on Encapsulated Magnetic Nanoparticles for CO₂ Sequestration

Carbonic Anhydrase Immobilized on Encapsulated Magnetic Nanoparticles for CO₂ Sequestration