A kinetic modeling of chondrocyte culture for manufacture of tissue-engineered cartilage

Kino‐oka, Masahiro; Maeda, Yasuhiko; Yamamoto, Takeyuki; Sugawara, Katsura; Taya, Masahito

doi:10.1263/jbb.99.197

Cited by 51 publications

(24 citation statements)

References 63 publications

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“…Successful tissue engineering requires healthy expandable cells, a porous biocompatible scaffold, biochemical growth factors, and a favorable physiological environment for tissue regeneration (6,7). Cell sources, based on their origin, fall into three categories: autologous cells from the patient, allogeneic cells from another human donor, and xenogeneic cells from other species.…”

Section: Principles Of Tissue Engineeringmentioning

confidence: 99%

Monitoring Tissue Engineering Using Magnetic Resonance Imaging

Othman

Magin

2008

Journal of Bioscience and Bioengineering

110

102

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Section: Principles Of Tissue Engineeringmentioning

confidence: 99%

Monitoring Tissue Engineering Using Magnetic Resonance Imaging

Othman

Magin

2008

Journal of Bioscience and Bioengineering

110

102

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“…In this study, we isolated adipose-derived stromal vascular fraction culture cells (ADSVFs) using mesenchymal stem cell markers, and investigated the differentiation of highly pure adipose-derived stem cells (ASCs) with no contaminating vascular endothelial cells or fibroblasts into chondrocytes (Halvorsen et al 2001;Kino-oka et al 2005;Xu et al 2007). …”

Section: Flow Cytometric Analysismentioning

confidence: 99%

Differentiation of Adipose-derived Stromal Vascular Fraction Culture Cells into Chondrocytes Using the Method of Cell Sorting with a Mesenchymal Stem Cell Marker

Ishimura

Yamamoto

Tajima

et al. 2008

Tohoku J. Exp. Med.

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The incidence of arthritic diseases is rapidly increasing in most advanced countries. Articular cartilage, which is the most important tissue in the joint, consists of chondrocytes and abundant extracellular matrix, including aggrecan, and shows poor self-repair. We studied the potential of stem cells in mouse subcutaneous adipose tissue as a source of cells to regenerate cartilage tissue. Analysis of adipose-derived stromal vascular fraction culture cells (ADSVFs) using mesenchymal stem cell markers showed that CD90-positive cells accounted for 93.8%, CD105-positive cells for 68.5%, and p75 neurotrophin receptor (p75NTR, CD271)-positive cells for 36.1%. These results indicate that cells positive for mesenchymal stem cell markers are present in ADSVFs. The CD105-positive or -negative cells were isolated from ADSVFs by magnetic cell separation (MACS), and the efficiency of differentiation into chondrocytes was compared with using three methods of pellet method, gel-coating method, and gel-embedding sheet method. Using the CD105-positive cells and the gel-embedding sheet method, aggrecan mRNA was detected about three times higher than pellet and gel-coating methods. The above data suggest that ADSVFs could be differentiated into chondrocyte-like cells in the gel-embedding sheet method and could be useful in regenerative medicine to treat cartilage defects or cartilage degenerative disease. The use of cells sorted by mesenchymal stem cell markers from adipose tissue would gain position in the repair of cartilage tissue.chondrocyte; adipose-derived stromal vascular fraction culture cells (ADSVFs); CD105; mesenchymal stem cell; gel-embedding sheet.

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“…6,7 The ideal scaffold materials for the engineering of cartilage should be biodegradable, processable, and with biomimetic structure. Many natural and synthetic materials have been considered, such as fibrin, alginate, chitosan, hyaluronan, collagen, polylactic acid (PLA), and poly lactate glycolic acid (PLGA).…”

Section: Introductionmentioning

confidence: 99%

Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel

Yuan

Zhang

et al. 2010

Biotechnology Progress

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Allogeneic mesenchymal stem cells (MSCs) are regarded as promising seed cells for engineering cartilage. However, few researches have covered the immune properties of seeded MSCs. Collagen has been considered as good scaffold, whether it has inherent chondrogenic inducibility for MSCs is still in debate. In this study, engineering grafts are constructed by neonatal rabbit MSCs and collagen Type I hydrogel. After periods of culture, the appearance of chondroid tissue in the grafts and the cartilage matrix-specific genes expressions of seeded cells prove the inducibility of collagen hydrogel, even if the growth factors are absence. With the differentiation, immunological properties of MSCs are changing. The expressions of main histocompatibility complex (MHC) molecules increase and the ability to inhibit the proliferation of activated lymphocytes may be declined. But to a large extent, it keeps the low stimulating to allogeneic lymphocytes and the small absolute value of MHCs. The changes are adverse for avoiding inflammation and rejection. Therefore, suitable scaffold and engineering strategies should be selected. For the grafts based on Collagen I hydrogel and MSCs, a longer culture period might not be necessary. To maintain the immune regulation, a higher initial MSCs density in engineering grafts may be more meaningful.

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A kinetic modeling of chondrocyte culture for manufacture of tissue-engineered cartilage

Cited by 51 publications

References 63 publications

Monitoring Tissue Engineering Using Magnetic Resonance Imaging

Monitoring Tissue Engineering Using Magnetic Resonance Imaging

Differentiation of Adipose-derived Stromal Vascular Fraction Culture Cells into Chondrocytes Using the Method of Cell Sorting with a Mesenchymal Stem Cell Marker

Chondrogenic differentiation and immunological properties of mesenchymal stem cells in collagen type I hydrogel

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