A kinetic method for assay of plant peroxidase and catalase activities by chemiluminescence

Frébort, Ivo; Haviger, Antonín; Jı́lek, Milan

doi:10.1002/pca.2800030203

Cited by 5 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Once the samples had been powdered, the powder was added to 1 mL of 50 mM sodium-phosphate buffer (pH 7) containing 2 mM dithiothreitol, 2 mM EDTA, 0.2% Triton X-100, and 50 mM Tris-HCl. The resultant sample was centrifuged at 12,000 rpm for 30 min at 4 • C, and the supernatant was used for measurement of CAT and APX activities [38]. Soluble protein was determined using bovine serum albumin (BSA) at 595 nm by a UV/VIS spectrophotometer according to the method described by Bradford [39].…”

Section: Antioxidant Enzyme Activitymentioning

confidence: 99%

Physiological, Biochemical, and Agronomic Trait Responses of Nigella sativa Genotypes to Water Stress

et al. 2022

View full text Add to dashboard Cite

Water stress may affect the growth, physiology, morphology, biochemistry, and productivity of Nigella sativa (black cumin), a medicinal and aromatic plant. Measuring these parameters under various irrigation regimes could provide useful information for successful genotype selection and breeding. Therefore, these agronomically significant features were evaluated in ten black cumin genotypes (Afghanistan, Pakistan, Syria, India, Arak, Isfahan, Semirom, Shahreza, Shahrekord, and Mashhad) under three irrigation regimes (40% (I1), 60% (I2), and 80% (I3) of permissible moisture discharge) during the 2017 to 2018 growing seasons. Water stress was shown to increase the levels of carotenoids (Cars), proline, total soluble carbohydrates (TSC), malondialdehyde (MDA), hydrogen peroxide (H2O2), catalase (CAT), and ascorbate peroxidase (APX) activities but reduced the relative water content (RWC) and chlorophyll content. The highest increases in Cars, TSC, proline, CAT, and APX were noted in the Arak, Isfahan, Semirom, Shahreza, Shahrekord, and Mashhad genotypes under the I3 water regime, respectively. At the same time, the lowest decrease was observed in chlorophyll, H2O2, and relative water content (RWC) in Semirom. According to the stress susceptibility index, the most resistant genotypes were Shahrekord under I2 and Semirom under I3. These data demonstrate that the irrigation regimes affected the physiological, biochemical, and morphological features of black cumin both qualitatively and quantitatively, although the impact varied depending upon the genotype, irrigation regime, and traits. As such, the results presented represent valuable information with which to inform future selection and breeding programs for drought-tolerant black cumin. This is of particular significance considering global climate change.

show abstract

Section: Antioxidant Enzyme Activitymentioning

confidence: 99%

Physiological, Biochemical, and Agronomic Trait Responses of Nigella sativa Genotypes to Water Stress

et al. 2022

View full text Add to dashboard Cite

show abstract

“…5,6) This enzyme has also been used to study the role of reactive oxygen species (ROS) involved in gene expression and apoptosis. 7,8) Although catalase was one of the first enzymes to be isolated and purified, its physiological function and regulation are not yet fully understood.…”

mentioning

confidence: 99%

Chemical Structure-Dependent Differential Effects of Flavonoids on the Catalase Activity as Evaluated by a Chemiluminescent Method

Doronicheva

Yasui

2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The antioxidative activity of flavonoids depends upon a combination of many factors, such as the concentration and chemical structure of the flavonoids and the arrangement of functional groups in their structure. In the present study, to evaluate the antioxidative effect of several types of flavonoids on catalase activity at a physiological H 2 O 2 concentration, a chemiluminescent (CL) method was used. The H 2 O 2 /luminol-dependent CL intensity in a system containing 3.7 nM catalase and low concentrations (10-100 nM) of green tea flavanols (epigallocatechin gallate; EGCG and epicatechin gallate; EG) was enhanced in comparison with that of a system without catalase, suggesting that EGCG and EG partially suppressed catalase activity. On the other hand, flavone and flavonols such as rutin (a 3-glycosidic flavone), quercitrin (a 3-glycosidic flavonol), myricetin, and kaempferol (flavonols), respectively, lowered the CL intensity to a greater extent at low concentrations (Ͻ0.1 m mM) when catalase was present than when catalase was absent, indicating that these flavonoids activate catalase. In addition, isoflavone and flavanone such as daidzein and naringenin, respectively, exhibited weak antioxidative activities against H 2 O 2 without any effect on the catalase activity over a wide range of flavonoid concentrations (0.04-0.4 m mM). From these results, it was for the first time suggested that the binding of flavonoids to the heme moiety or a protein region of catalase contributes to the enhancement of catalase activity.

show abstract

Exogenous sulfur application can effectively alleviate iron deficiency yellowing in peanuts and increase pod yield

Zhang,

Hou,

Gou

et al. 2024

European Journal of Agronomy

View full text Add to dashboard Cite

A kinetic method for assay of plant peroxidase and catalase activities by chemiluminescence

Cited by 5 publications

References 11 publications

Physiological, Biochemical, and Agronomic Trait Responses of Nigella sativa Genotypes to Water Stress

Physiological, Biochemical, and Agronomic Trait Responses of Nigella sativa Genotypes to Water Stress

Chemical Structure-Dependent Differential Effects of Flavonoids on the Catalase Activity as Evaluated by a Chemiluminescent Method

Exogenous sulfur application can effectively alleviate iron deficiency yellowing in peanuts and increase pod yield

Contact Info

Product

Resources

About