A kinetic analysis of the action of the insect prothoracicotropic hormone

Amplification and expression of a salivary gland DNA puff gene in the prothoracic gland of Bradysia hygida (Diptera: Sciaridae)

“…After incubation, the tissues were removed and the medium was transferred to an Eppendorf tube and stored at À20°C until further processing. The amount of ecdysone released during the incubation time was determined by radioimmunoassay (RIA), as previously described (Basso et al, 2002;Hartfelder et al, 2000), using an antiserum prepared against a hemisuccinate derivative of ecdysone (Bollenbacher et al, 1983;Warren and Gilbert, 1986) and [23, H(N)] ecdysone (Perkin Elmer) (NEN, spec. act.…”

Developmental characterization, function and regulation of a Laccase2 encoding gene in the honey bee, Apis mellifera (Hymenoptera, Apinae)

“…As only small quantities of hemolymph could be collected from individual bees, the hemolymph from ligated and non-ligated bees were pooled to make up 2 ml samples, which is an adequate volume for hormone detection under the assay conditions used. Ecdysteroid titers were quantified by a radioimmunoassay (RIA), as previously described (Feldlaufer and Hartfelder, 1997;Bloch et al, 2000), using an antiserum prepared against an ecdysone derivative (Bollenbacher et al, 1983;Warren and Gilbert, 1986) at a final concentration of 0.05%, and using [23,24-3H(N)]ecdysone (NEN, spec. act.…”

The release of an insect neuropeptide hormone, bombyxin, is regulated by muscarinic transmission in the brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori (Lepidoptera: Bomby cidae).

“…PTTH is produced in two pairs of dorsolateral neurosecretory cells and released from the end of the axon at the corpus-allatum (Mizoguchi et al, 1990). The released PTTH stimulates the prothoracic gland to synthesize and secrete ecdysone (Bollenbacher et al, 1979;Agui et al, 1980). The change in ecdysone level results in the molt, and ecdysis is triggered by eclosion hormone (Truman, 1985).…”