A kinetic analysis of d-xylose transport in rhodotorula glutinis

Alcorn, Mary E.; Griffin, Charles C.

doi:10.1016/0005-2736(78)90036-6

Cited by 41 publications

(12 citation statements)

References 14 publications

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“…However, it would be beneficial to express an active xylose transporter, which is capable of taking up xylose in the cell against a concentration gradient, for utilization of xylose below 1 g L −1 (6.7 mM). Such transporters are common among the natural xylose utilizing yeasts (Alcorn and Griffin, 1978;Does and Bisson, 1989;Kilian and van Uden, 1988;Kilian et al, 1993;Lucas and van Uden, 1986;Nobre et al, 1999;Weierstall et al, 1999). In addition, it would be desirable to express a transporter, which is specific for xylose and not inhibited by glucose.…”

Section: Discussionmentioning

confidence: 99%

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Gárdonyi

Jeppsson

Lidén

et al. 2003

Biotech & Bioengineering

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Section: Discussionmentioning

confidence: 99%

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Gárdonyi

Jeppsson

Lidén

et al. 2003

Biotech & Bioengineering

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“…A similar high affinity, accumulative xylose-proton symport, has been found to occur in species of the yeast genus Rhodotorula in addition to low affinity facilitated diffusion. As in C. shehatae the high affinity system of Rhodotorula also accepted Dgalactose while the low affinity system did not (H6fer 1970;Janda et al 1976;H6fer and Misra 1978;Alcorn and Griffin 1978).…”

Section: Transport Of D-xylose In Derepressed Cellsmentioning

confidence: 93%

Transport of hemicellulose monomers in the xylose-fermenting yeastCandida shehatae

Lucas

Uden

1986

Appl Microbiol Biotechnol

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Summary. Cells of Candida shehatae repressed by growth in glucose-or D-xylose-medium produced a facilitated diffusion system that transported glucose ( K s + 2 m M , Vm,x--+2.3 mmoles g-1 h-l), D-xylose (Ks + 125 mM, Vmax + 22.5 mmoles g-1 h-1) and D-mannose, but neither D-galactose nor L-arabinose.Cells derepressed by starvation formed several sugar-proton symports. One proton symport accumulated 3-0-methylglucose about 400-fold and transported glucose (Ks+0.12 mM, V m a x + 3 . 2 mmoles g-1 h-1) and D-mannose, a second proton symport transported D-xylose (Ks+ 1.0 mM, Vmax 1.4 mmoles g-1 h-1) and D-galactose, while L-arabinose apparently used a third proton symport. The stoicheiometry was one proton for each molecule of glucose or D-xylose transported. Substrates of one sugar proton symport inhibited non-competitively the transport of substrates of the other symports.Starvation, while inducing the sugar-proton symports, silenced the facilitated diffusion system with respect to glucose transport but not with respect to the transport of D-xylose, facilitated diffusion functioning simultaneously with the D-xylose-proton symport.

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“…the low-affinity electroneutral component of D-xylose transport which became evident at pH values above 6 and at high substrate concentrations (H6fer & Misra, 1978;Hauer & H6fer, 1982). However, this carrier system so far failed to accept any substrate other than D-xylose (unpublished results), thus representing obviously a different molecular entity of R. gracilis plasma membrane as concluded by Alcorn and Griffin (1978). This work has been supported by the Deutsche Forschungsgemeinschafl (grant no.…”

Section: Glucuronate Is Translocated By the Monosaccharide Carrier Wimentioning

confidence: 78%

Transport of an anionic substrate by the H+/monosaccharide symport inRhodotorula gracilis: Only the protonated form of the carrier is catalytically active

Niemietz

Höfer

1984

J. Membrain Biol.

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Summary. The yeast Rhodotorula gracilis accumulated glucuronate by an H +/symport. The transport was electroneutral, driven by the chemical gradient of protons A pH. The observed sloichiometry amounted to I proton per molecule glucuronate. At pH 4. the half-saturation constant K-~ was at its lowest value (K; = g raM). whereas the maximal velocity V, reached a maximum( V; 15 nmol/min x mg dry wt). Monosaccharides competitively inhinted the uptake ofglucuronate and vice versa. Hence, the two substrates share the same transport system. The steady-state accumulation of glucuronate reflected the course of the pH gradient. It is concluded Ibat glucuronate is transported as an anionic substrate by lhe prolonated carrier, the driving force being the chemical gradient of the H + (ApH). The ternary carrier/H+/glc -COO -complex is electroneutral and independent of the membrane potential. Simultaneous uptake of organic acids (acetic or propionic acid) which is also energized by the pH gradient ted to a noncompetitive inhibition of glucuronate transport. Thus, manipulation of the driving force, ApH. reduced V; without affecting K;. Kinetic and energetic arguments are presented which stronty suggest lhal only the protonated carrier is catalytically active in R. ~racilis.

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A kinetic analysis of d-xylose transport in rhodotorula glutinis

Cited by 41 publications

References 14 publications

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Transport of hemicellulose monomers in the xylose-fermenting yeastCandida shehatae

Transport of an anionic substrate by the H+/monosaccharide symport inRhodotorula gracilis: Only the protonated form of the carrier is catalytically active

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