A Kinesin Driven Enzyme Linked Immunosorbant Assay (ELISA) for Ultra Low Protein Detection Applications

Abstract

Fast efficient chromatography is important in order to resolve the individual components of complex biological mixtures. Classically, thermal effects that decrease chromatographic efficiency accompany both HPLC and SFC. In

Artificial Molecular Machines

“…Kinesin, myosin, and dynein systems have been much more extensively utilized. − They have been shown to transport cargos in artificial systems, although cargos must typically be large to outcompete diffusive forces. ,− These biological walkers have been controlled by the design of appropriate tracks, − or by the application of external forces. Kinesin has been used to stretch a length of DNA, and to enable the detection of nanomolar concentrations of a targeted protein . Cross-linked actin and myosin gels were found to move over each other in the presence of ATP .…”

“…Kinesin has been used to stretch a length of DNA, 1715 and to enable the detection of nanomolar concentrations of a targeted protein. 1731 Cross-linked actin and myosin gels were found to move over each other in the presence of ATP. 1732 Microtubule containing gels combined with kinesin and ATP have been shown to spontaneously generate autonomous motility.…”

Microtubule Detachment in Gliding Motility Assays Limits the Performance of Kinesin-Driven Molecular Shuttles

“…In a device such as the “smart dust biosensor”, where gliding microtubules capture, tag, and deposit an analyte, it limits the start-to-end distance covered in the process. In a device for analyte concentration, it limits the harvesting area. − In a biocomputation device, it may cause an exponentially increasing loss of motile agents which compromises the accuracy of the output. The average gliding distance before detachment may be increased by utilizing higher motor densities, although higher motor densities have negative effects too, such as higher energy consumption and higher rates of microtubule shrinking .…”