A-kinase interacting protein 1 is sufficiently expressed and positively associates with WHO grade, meanwhile predicts unfavorable overall survival independently in glioma patients

Abstract

The present study aimed to investigate the association of A-kinase interacting protein 1 (AKIP1) with clinical characteristics, and further explore the prognostic value of AKIP1 in glioma patients. Totally 168 glioma patients who underwent tumor resection were analyzed, and their tumor tissue specimens were acquired for the detection of AKIP1 expression by immunohistochemistry (IHC), which was scored by a semi-quantitative method considering staining intensity and staining density. According to AKIP1 expression in tumor tissues of glioma patients, there were 65 (38.7%) patients with AKIP1 low expression (IHC score 0–3), 48 (28.6%) patients with AKIP1 high + expression (IHC score 4–6), 42 (25.0%) patients with AKIP1 high++ expression (IHC score 7–9) and 13 (7.7%) patients with AKIP1 high+++ expression (IHC score 10–12), respectively. AKIP1 expression was positively associated with World Health Organization grade. Overall survival (OS) was the lowest in the patients with AKIP1 high+++ expression, followed by those with AKIP1 high++ expression and those with AKIP1 high+ expression, and highest in those with AKIP1 low expression. Further subgroup analysis exhibited that AKIP1 expression was negatively associated with OS especially in high-grade glioma patients. In addition, AKIP1 expression was negatively associated with OS in all subgroups of patients with/without adjuvant radiotherapy, with/without adjuvant chemotherapy. Further multivariate Cox's regression exhibited that AKIP1 high expression was an independent predictive factor for worse OS. AKIP1 presents with the potential to be a novel biomarker for tumor management and prognosis surveillance in glioma patients.

Silencing of AKIP1 Suppresses the Proliferation, Migration, and Epithelial-Mesenchymal Transition Process of Glioma Cells by Upregulating DLG2

“…It also played an essential role in esophageal squamous cell BioMed Research International carcinoma and could predict the poor prognosis of breast cancer via Akt/GSK-3β/Snail pathway [14,15]. The latest statistics, which illustrated the negative association of AKIP1 with overall survival of glioma patients, elucidated the potential of AKIP1 to be a novel biomarker for the treatment of glioma [6]. In the present study, overexpressed AKIP1 was also noted in a myriad of glioma cell lines, which was consistent with the public recognition that AKIP1 might exert an oncogenic role in tumor cells.…”

“…A kinase-interacting protein 1 (AKIP1), a 23 kDa protein, was originally discovered in breast and prostate cancer cell lines by mRNA screens [ 5 ]. Recent report has considered AKIP1 as an independent predictor factor for malignant glioma by virtue of high AKIP1 expression presented in most of glioma patients [ 6 ]. CCLE website predicted that the expression of AKIP1 was upregulated in glioma cells.…”

Chaperonin‐containing tailless complex polypeptide 1 subunit 6A correlates with increased World Health Organization grade, less isocitrate dehydrogenase mutation, and deteriorative survival of astrocytoma patients

“…The diaminobenzidine (DAB; Sigma‐Aldrich) was used for staining, and hematoxylin (Sigma‐Aldrich) was used for counterstain. The intensity and density of IHC staining were recorded under a light microscope using a semi‐quantitative scoring method as previous report 18 . Two investigators evaluated the intensity and density of IHC staining independently, and the final value of intensity and density of IHC staining was calculated as the mean of two investigators.…”

“…The intensity and density of IHC staining were recorded under a light microscope using a semi-quantitative scoring method as previous report. 18 Two investigators evaluated the intensity and density of IHC staining independently, and the final value of intensity and…”

The relationship among integrin alpha 7, CD133 and Nestin as well as their correlation with clinicopathological features and prognosis in astrocytoma patients

“…At the end of IHC assay, the staining results of sections were viewed and photographed using a microscope. The intensity and density of the IHC staining were scored by two experts, and the detailed scoring procedures were performed in terms of the method described in the published literature [23,9,24]. The IHC score was the product of intensity score and density score, ranging from 0 to 12.…”