A key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation

Zhang, Chengcheng; Yu, Leilei; Ma, Chenchen; Jiang, Shuaiming; Zhang, Yufeng; Wang, Shunhe; Tian, Fengwei; Xue, Yuzheng; Zhao, Jianxin; Zhang, Hao; Liu, Liming; Chen, Wei; Huang, Shi; Zhang, Jiachao; Zhai, Qixiao

doi:10.1016/j.chom.2023.10.011

Cited by 11 publications

(2 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Referring to previous methods, fecal DNA extraction, 16S rRNA sequencing, and bioinformatics analysis were performed to investigate the composition and structure of the gut microbiota, respectively. 48 The functions vegdist (method = “bray”) and cmdscale of the vegan package in R v 4.2.2 were used to perform principal coordinate analysis based on the Bray–Curtis distance. Linear discriminant analysis (LDA) effect size (LEfSe) analysis was performed to identify significantly different bacteria among groups.…”

Section: Methodsmentioning

confidence: 99%

Analysis of the key genes of Lactobacillus reuteri strains involved in the protection against alcohol-induced intestinal barrier damage

Zhang,

et al. 2024

Food Funct.

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Analysis of the key genes of Lactobacillus reuteri strains involved in the protection against alcohol-induced intestinal barrier damage

Zhang,

et al. 2024

Food Funct.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Metabolite analyses of CKT and GT were conducted and analyzed by Novogene Co., Ltd. The method used to measure the metabolomics was consistent with that described in the research (Zhang et al, 2023).…”

Section: Metabolomicsmentioning

confidence: 99%

An Ilex latifolia‐containing compound tea regulates glucose–lipid metabolism and modulates gut microbiota in high‐fat diet‐fed mice

Xu,

Cheng,

Wen

et al. 2024

Food Frontiers

View full text Add to dashboard Cite

Kuding Tea (Ilex latifolia) is a bitter‐tasting herbal tea that was used for the treatment of symptoms related with diabetes mellitus, hypertension, and hyperlipidemia. However, Kuding Tea is also difficultly accepted by people in daily life because of its poor palatability. In this study, Kuding Tea, green tea (GT) (Camellia sinensis L.), and Luohan (Siraitia grosvenorii) fruits were formulated into a compound Kuding Tea (CKT) to improve the taste and health benefits of this beverage. High‐fat diet‐fed male C57BL/6J mice were used as animal models to explore the effects of CKT (6 or 12 mg/mL, water ad libitum) on body weight, food intake, liver function, blood glucose and lipids, and gene expression. L02 and 3T3‐L1 cells were used to further demonstrate the effects of CKT on fat accumulation and hepatic lipid deposition. Our results suggest that CKT can regulate glucose and lipid metabolism by decreasing body weight, reducing white adipose deposition, improving glucose tolerance, increasing the expression of brown adipose genes, and reducing fat accumulation in the liver, and CKT inhibited fat accumulation better than GT. In addition, a low dose (6 mg/mL) of CKT reduced the abundance of Desulfovibrio bacteria, positively associated with obesity, and increased that of norank_f__Muribaculaceae, Lachnospiraceae_NK4A136_group, and Alloprevotella, which are beneficial to glucose and lipid metabolism. This study suggests that CKT not only has a better palatability but also has potential preventive effects on high‐fat diet‐induced glucose–lipid metabolic diseases.

show abstract

A Novel Multiple-Network Analysis Integrating Metabolomics and Pharmacology for Mechanism Study of Medicine: A Case Study of CDD-2101 Against Constipation

Shi,

Xiang,

Lam

et al. 2024

J. Anal. Test.

View full text Add to dashboard Cite

Constipation affects more than 14% of the global population with complex pathogenesis. Compound Traditional Chinese medicines (TCM), such as CDD-2101, a manufactured version of a time-tested TCM, hold great promise for constipation treatment due to the multiple-component-multiple-target strategy. Here, a novel multiple network analysis based on metabolomics and network pharmacology was developed to decipher the complex interplays between CDD-2101 and constipation. First, CDD-2101 alleviated constipation in a loperamide-induced acute constipation mice model and no adverse effect was observed. Then, fecal metabolomics and phytochemistry profiles were conducted by liquid chromatography-mass spectrometry analysis. The untargeted metabolomics built a metabolomic network related to significantly modulated pathways during the constipation alleviation, including bile acid secretion, steroid hormone biosynthesis, glycerophospholipid metabolism, and linoleic acid metabolism. Based on the phytochemistry, network pharmacology predicted similar modulated pathways and built a hub-target network including retinoid X receptor alpha (RXRA), cytochrome P450 1A1 (CYP1A1), cytochrome P450 1A2 (CYP1A2), and phospholipase A2-IVA (PLA2G4). Third, integrated networks were built to select the key pathways and targets, as well as the key ingredients, including rhein, hesperetin, albiflorin, and magnolol from the TCM, which were corroborated by molecular docking, in vitro enzyme assays, and targeted metabolomics results. Finally, an integrated network was built to elucidate the multi-ingredient-multi-symptom linkage during the treatment. In conclusion, the novel multiple-network analysis revealed the complex “multi-component-multi-target-multi-pathway” mechanisms of CDD-2101 against constipation. Our work provides insight into the understanding of active compounds and pharmacological mechanisms, contributing to the development of natural medicines.

show abstract

A key genetic factor governing arabinan utilization in the gut microbiome alleviates constipation

Cited by 11 publications

References 82 publications

Analysis of the key genes of Lactobacillus reuteri strains involved in the protection against alcohol-induced intestinal barrier damage

Analysis of the key genes of Lactobacillus reuteri strains involved in the protection against alcohol-induced intestinal barrier damage

An Ilex latifolia‐containing compound tea regulates glucose–lipid metabolism and modulates gut microbiota in high‐fat diet‐fed mice

A Novel Multiple-Network Analysis Integrating Metabolomics and Pharmacology for Mechanism Study of Medicine: A Case Study of CDD-2101 Against Constipation

Contact Info

Product

Resources

About