A key ‘foxy’ aroma gene is regulated by homology-induced promoter indels in the iconic juice grape ‘Concord’

Yang, Yingzhen; Cuenca, José; Wang, Nian; Liang, Zhenchang; Sun, Honghe; Gutierrez, Benjamin; Xi, Xiaojun; Arro, Jie; Wang, Yi; Fan, Peige; Londo, Jason P.; Cousins, Peter; Li, Shaohua; Fei, Zhangjun; Zhong, Gan-Yuan

doi:10.1038/s41438-020-0304-6

Cited by 19 publications

(15 citation statements)

References 54 publications

(76 reference statements)

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“…Although SNPs Y158 and K217 are two intronic mutations with presumably little effect on VviUCC1 function ( Figure 1 ), two associated SNPs (Y-984 and K-88) were found to affect two common cis -transcriptional regulatory elements (a CAAT-box and a TATA-box, respectively) ( Figure S2 ). It suggests a possible role of Y-984 and K-88 in the modulation of VviUCC1 expression, as recently reported for a series of INDELs located in the anthraniloyl-CoA:methanol acyltransferase gene promoter affecting methyl anthranilate accumulation and ‘foxy’ aroma in Concord grapes [ 80 ]. Of special interest is K-88, located ca .…”

Section: Discussionsupporting

confidence: 64%

VviUCC1 Nucleotide Diversity, Linkage Disequilibrium and Association with Rachis Architecture Traits in Grapevine

Tello

Torres‐Pérez

Flutre

et al. 2020

Genes

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Cluster compactness is a trait with high agronomic relevance, affecting crop yield and grape composition. Rachis architecture is a major component of cluster compactness determinism, and is a target trait toward the breeding of grapevine varieties less susceptible to pests and diseases. Although its genetic basis is scarcely understood, a preliminary result indicated a possible involvement of the VviUCC1 gene. The aim of this study was to characterize the VviUCC1 gene in grapevine and to test the association between the natural variation observed for a series of rachis architecture traits and the polymorphisms detected in the VviUCC1 sequence. This gene encodes an uclacyanin plant-specific cell-wall protein involved in fiber formation and/or lignification processes. A high nucleotide diversity in the VviUCC1 gene promoter and coding regions was observed, but no critical effects were predicted in the protein domains, indicating a high level of conservation of its function in the cultivated grapevine. After correcting statistical models for genetic stratification and linkage disequilibrium effects, marker-trait association results revealed a series of single nucleotide polymorphisms (SNPs) significantly associated with cluster compactness and rachis traits variation. Two of them (Y-984 and K-88) affected two common cis-transcriptional regulatory elements, suggesting an effect on phenotype via gene expression regulation. This work reinforces the interest of further studies aiming to reveal the functional effect of the detected VviUCC1 variants on grapevine rachis architecture.

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Section: Discussionsupporting

confidence: 64%

VviUCC1 Nucleotide Diversity, Linkage Disequilibrium and Association with Rachis Architecture Traits in Grapevine

Tello

Torres‐Pérez

Flutre

et al. 2020

Genes

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“…Interestingly, the homologous genes VvSTS and VpSTS from V. vinifera “Carigane” and V. pseudoreticulata share >99% identity on the amino acid level, but with significant difference in promoter regions; VpSTS conferred powdery mildew resistance with an elevated responsiveness of the promoter [ 46 ]. In V. labrusca “Concord”, the absence of a 426 bp and/or a 42 bp sequence in the acyltransferase gene (AMAT) promoter highly associated with high levels of AMAT expression, and further regulated the methyl anthranilate (MA) accumulation, which caused a special “foxy” aroma [ 47 ]. We claim that promoter differentiation between VvWhy1 and VpWhy1 may lead to disease resistance differentiation in V. vinifera and V. pseudoreticulata.…”

Section: Discussionmentioning

confidence: 99%

The Single-Stranded DNA-Binding Gene Whirly (Why1) with a Strong Pathogen-Induced Promoter from Vitis pseudoreticulata Enhances Resistance to Phytophthora capsici

Lai

Que

Pan

et al. 2022

IJMS

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Vitis vinifera plants are disease-susceptible while Vitis pseudoreticulata plants are disease-resistant; however, the molecular mechanism remains unclear. In this study, the single-stranded DNA- and RNA-binding protein gene Whirly (VvWhy1 and VpWhy1) were cloned from V. vinifera “Cabernet Sauvignon” and V. pseudoreticulata “HD1”. VvWhy1 and VpWhy1 promoter sequences (pVv and pVp) were also isolated; however, the identity of the promoter sequences was far lower than that between the Why1 coding sequences (CDSs). Both Why1 gene sequences had seven exons and six introns, and they had a C-terminal Whirly conserved domain and N-terminal chloroplast transit peptide, which was then verified to be chloroplast localization. Transcriptional expression showed that VpWhy1 was strongly induced by Plasmopara viticola, while VvWhy1 showed a low expression level. Further, the GUS activity indicated pVp had high activity involved in response to Phytophthora capsici infection. In addition, Nicotiana benthamiana transiently expressing pVp::VvWhy1 and pVp::VpWhy1 enhanced the P. capsici resistance. Moreover, Why1, PR1 and PR10 were upregulated in pVp transgenic N. benthamiana leaves. This research presented a novel insight into disease resistance mechanism that pVp promoted the transcription of Why1, which subsequently regulated the expression of PR1 and PR10, further enhancing the resistance to P. capsici.

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“…The leading cultivars used by the American juice industry are 'Isabella', 'Bordo/ Ives', and 'Concord', because of their high productivity, resistance to diseases, nutritional properties, and unique flavors. 11 However, these genotypes exhibit uneven maturation, weak coloration, low content of soluble solids, and poor adaptation to warmer climates. 4 The grapevine breeding program at Embrapa has been continually developing adapted cultivars by clonal selection and controlled crosses.…”

Section: Introductionmentioning

confidence: 99%

“…In Europe, grape juices are made from berries of winemaking V. vinifera cultivars, 4 whereas in North and South America juices are mainly made from grapes of V. labrusca or V. vinifera × V. labrusca interspecific hybrids. The leading cultivars used by the American juice industry are ‘Isabella’, ‘Bordo/Ives’, and ‘Concord’, because of their high productivity, resistance to diseases, nutritional properties, and unique flavors 11 . However, these genotypes exhibit uneven maturation, weak coloration, low content of soluble solids, and poor adaptation to warmer climates 4…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiling reveals the influence of grapevine genetic distance on the chemical signature of juices

Gomez,

Niederauer,

Minatel

et al. 2023

J Sci Food Agric

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BackgroundYield, disease tolerance, and climate adaptation are important traits in grapevine genetic breeding programs. Selection for these characteristics cause unpredictable changes in primary and specialized metabolism, affecting the physicochemical properties and chemical composition of the berries and their processed products, juice and wine. In this study, we investigated the influence of the genetic distance between grapevine genotypes on the chemical signatures of the juices, by integrating comprehensive metabolic profiling to genetic analyses.ResultsThe studied grapevine cultivars exhibited low genetic diversity. Breeding for agronomic traits promoted higher contents of soluble sugars, total phenolics, and anthocyanins in the juices. Untargeted juice metabolomics identified a total of 147 metabolites, consisting of 30 volatiles, 21 phenolics, and 96 UHPLC‐MS features. Juices from grapes of the most recent cultivars exhibited increased levels of trans‐resveratrol, catechin, and luteolin. The blend of volatiles from juices of later cultivars was also more complex, consisting of 29 distinct metabolites in ‘BRS Magna. Grapes from ‘BRS Carmem’, an intermediate cultivar, gave the most divergent UHPLC‐MS juice profile.ConclusionContents of soluble solids, total phenolics and anthocyanins in grape juices were increased by controlled crosses and hybrid selection. Integrative analyses demonstrated that the juices metabolic profiles accurately represent the cultivars genetic distances. Juices from ‘BRS Violeta’ and ‘BRS Magna’ show relevant positive association with health‐related phenolics and a distinct set of odor volatiles, although these characteristics were not aimed at by breeding.This article is protected by copyright. All rights reserved.

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A key ‘foxy’ aroma gene is regulated by homology-induced promoter indels in the iconic juice grape ‘Concord’

Cited by 19 publications

References 54 publications

VviUCC1 Nucleotide Diversity, Linkage Disequilibrium and Association with Rachis Architecture Traits in Grapevine

VviUCC1 Nucleotide Diversity, Linkage Disequilibrium and Association with Rachis Architecture Traits in Grapevine

The Single-Stranded DNA-Binding Gene Whirly (Why1) with a Strong Pathogen-Induced Promoter from Vitis pseudoreticulata Enhances Resistance to Phytophthora capsici

Metabolite profiling reveals the influence of grapevine genetic distance on the chemical signature of juices

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