A Key ABA Catabolic Gene, OsABA8ox3, Is Involved in Drought Stress Resistance in Rice

Cai, Sanjun; Jiang, Guobin; Ye, Nenghui; Chu, Zhizhan; Xu, Xuezhong; Zhang, Jianhua; Zhu, Guiyu

doi:10.1371/journal.pone.0116646

Cited by 74 publications

(45 citation statements)

References 45 publications

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“…2B and 3, B and C) is similar to what was found for SDIR1, another RING-H2 protein implicated in the positive regulation of ABA signaling . A recent study showed that in rice, achieving higher ABA levels through down-regulation of OsABA8ox3 using an RNAi strategy can improve drought stress resistance (Cai et al, 2015). These results are consistent with our finding that ZmXerico1 reduces protein stability of specific ZmABA8ox in vivo, such as maize ortholog of OsABA8ox3, ZmABA8ox3a, leading to increased drought tolerance.…”

Section: Zmxerico1 Functions As An E3 Ubiquitin Ligase and Its Overesupporting

confidence: 92%

Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis

Brugière

Zhang

et al. 2017

Plant Physiol.

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Drought stress is one of the main environmental problems encountered by crop growers. Reduction in arable land area and reduced water availability make it paramount to identify and develop strategies to allow crops to be more resilient in waterlimiting environments. The plant hormone abscisic acid (ABA) plays an important role in the plants' response to drought stress through its control of stomatal aperture and water transpiration, and transgenic modulation of ABA levels therefore represents an attractive avenue to improve the drought tolerance of crops. Several steps in the ABA-signaling pathway are controlled by ubiquitination involving really interesting new genes (RING) domain-containing proteins. We characterized the maize (Zea mays) RING protein family and identified two novel RING-H2 genes called ZmXerico1 and ZmXerico2. Expression of ZmXerico genes is induced by drought stress, and we show that overexpression of ZmXerico1 and ZmXerico2 in Arabidopsis and maize confers ABA hypersensitivity and improved water use efficiency, which can lead to enhanced maize yield performance in a controlled drought-stress environment. Overexpression of ZmXerico1 and ZmXerico2 in maize results in increased ABA levels and decreased levels of ABA degradation products diphaseic acid and phaseic acid. We show that ZmXerico1 is localized in the endoplasmic reticulum, where ABA 89-hydroxylases have been shown to be localized, and that it functions as an E3 ubiquitin ligase. We demonstrate that ZmXerico1 plays a role in the control of ABA homeostasis through regulation of ABA 89-hydroxylase protein stability, representing a novel control point in the regulation of the ABA pathway.

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Section: Zmxerico1 Functions As An E3 Ubiquitin Ligase and Its Overesupporting

confidence: 92%

Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis

Brugière

Zhang

et al. 2017

Plant Physiol.

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“…ABA mediates plant germination and early seedling growth (Mulkey et al, 1983;Cornish and Zeevaart, 1985). Stimulating ABA biosynthesis inhibits seed germination as well as root and shoot growth in rice (Ikeda et al, 2002;Zhu et al, 2009;Cai et al, 2015). We found that seed germination was delayed in OE-OsDET1 plants and that root and shoot growth was inhibited during ABA treatment (Fig.…”

Section: Discussionmentioning

confidence: 75%

The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

et al. 2016

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DEETIOLATED1 (DET1) plays a critical role in developmental and environmental responses in many plants. To date, the functions of OsDET1 in rice (Oryza sativa) have been largely unknown. OsDET1 is an ortholog of Arabidopsis (Arabidopsis thaliana) DET1. Here, we found that OsDET1 is essential for maintaining normal rice development. The repression of OsDET1 had detrimental effects on plant development, and leaded to contradictory phenotypes related to abscisic acid (ABA) in OsDET1 interference (RNAi) plants. We found that OsDET1 is involved in modulating ABA signaling in rice. OsDET1 RNAi plants exhibited an ABA hypersensitivity phenotype. Using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation assays, we determined that OsDET1 interacts physically with DAMAGED-SPECIFIC DNA-BINDING PROTEIN1 (OsDDB1) and CONSTITUTIVE PHOTOMORPHOGENIC10 (COP10); DET1-and DDB1-ASSOCIATED1 binds to the ABA receptors OsPYL5 and OsDDB1. We found that the degradation of OsPYL5 was delayed in OsDET1 RNAi plants. These findings suggest that OsDET1 deficiency disturbs the COP10-DET1-DDB1 complex, which is responsible for ABA receptor (OsPYL) degradation, eventually leading to ABA sensitivity in rice. Additionally, OsDET1 also modulated ABA biosynthesis, as ABA biosynthesis was inhibited in OsDET1 RNAi plants and promoted in OsDET1-overexpressing transgenic plants. In conclusion, our data suggest that OsDET1 plays an important role in maintaining normal development in rice and mediates the cross talk between ABA biosynthesis and ABA signaling pathways in rice.

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“…Plant respond and adapt to drought stress through various physiological and metabolic processes including stomatal closure, repression cell growth, photosynthesis and activation of respiration to increase the chance of survival [33]. The molecular mechanism drought stress tolerance is extremely complicated which is a multigene phenomenon in Arabidopsis plants [34]. Signal transduction in response of drought stress can be divided in to two pathway, ABA independent and ABA dependent pathway [35].…”

Section: Drought Stress Responsementioning

confidence: 99%

RNAi Mediated Drought and Salinity Stress Tolerance in Plants

Pradhan¹,

Naik²,

Sahoo³

2015

AJPS

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RNAi mediated gene silencing demonstrated to serve as a defence mechanism against abiotic stress. Some endogenous small RNAs (microRNA and siRNA) have emerged as important players in plant abiotic stress response. Drought and salinity are the major environmental stresses that limit the agricultural food production. miRNA involved in drought and salinity stress response, including ABA response, auxin signalling, osmoprotection and antioxidant defence by downregulating the response target gene. It is observed that some of the microRNAs are upregulated or downregulated in response to drought and salt stress. We reviewed that miR167, miR393, mir474, miR169g are upregulated whereas miR168, miR396, miR397 are downregulated in rice plant during drought stress. Moreover, our detail categorical analysis on the basis of mechanism of action found that miRNA involved in drought stress was 28% in ABA signalling and response, 14.2% in auxin signalling, 9.52% in miRNA processing, 14.2% in cell growth, 9.52% in antioxidant defence, 4.76% in CO2 fixation and 9.52% in osmotic adjustment. Similarly, miRNA involved in salinity stress was 5.8% in auxin signalling, 23.5% in vegetative phase change and root, shoot, leaf and vascular development, 11.76% in gynoecium and stamens development, 8.82% in metabolic adaptation, 2.74% in early embryogenesis and 41.17% not known. Importantly, some common miRNAs such as miR159, miR167, miR169, miR393 and miR397 play an important role in both drought and salinity stress conditions. Here, in this review, we mainly focused on the current status of miRNAs, mechanism of action and their regulatory network during drought and salinity stress in plants.

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A Key ABA Catabolic Gene, OsABA8ox3, Is Involved in Drought Stress Resistance in Rice

Cited by 74 publications

References 45 publications

Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis

Overexpression of RING Domain E3 Ligase ZmXerico1 Confers Drought Tolerance through Regulation of ABA Homeostasis

The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

RNAi Mediated Drought and Salinity Stress Tolerance in Plants

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