A kaleidoscope of photosynthetic antenna proteins and their emerging roles

Arshad, Rameez; Saccon, Francesco; Bag, Pushan; Biswas, Avratanu; Calvaruso, Claudio; Bhatti, Ahmad Farhan; Grebe, Steffen; Mascoli, Vincenzo; Mahbub, Moontaha; Muzzopappa, Fernando; Polyzois, Alexandros; Schiphorst, Christo; Sorrentino, Mirella; Streckaitė, Simona; Amerongen, Herbert van; Aro, Eva‐Mari; Bassi, Roberto; Boekema, Egbert J.; Croce, Roberta; Dekker, Jan; Grondelle, Rienk van; Jansson, Stefan; Kirilovsky, Diana; Kouřil, Roman; Michel, Sylvie; Mullineaux, Conrad W.; Panzarová, Klára; Robert, Bruno; Ruban, Alexander V.; Stokkum, Ivo H. M. van; Wientjes, Emilie; Büchel, Claudia

doi:10.1093/plphys/kiac175

Cited by 16 publications

(11 citation statements)

References 196 publications

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“…About 80% of genes were detected in a genome scale in CX-26 middle leaves (Fig 2-3). Consistent with the reduction of SPAD value in T3 leaves, the DEGs of T1 and T2 leaves were fewer and PCA analysis showed an overlapped pattern protein complexes embedded in the thylakoid membrane in green plants [13]. At the same time, the gene expression between T2 and T3 leaves involved in carotenoid biosynthesis, porphyrin and chlorophyll metabolism and carbon fixation in photosynthetic organisms was altered (Fig 6).…”

Section: Keeg Analysis and Gene Regulatory Network Analysis Revealsupporting

confidence: 64%

“…Oxygenic photosynthesis is responsible for the great biomass of cigar tobacco leaves. The PS antenna proteins function in light capturing and energy transferring by binding Chla, Chlb, and carotenoids in the light-harvesting chlorophyll protein complexes embedded in the thylakoid membrane in green plants [13]. At the same time, the gene expression between T2 and T3 leaves involved in carotenoid biosynthesis, porphyrin and chlorophyll metabolism and carbon fixation in photosynthetic organisms was altered (Fig 6).…”

Section: Discussionmentioning

confidence: 99%

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Genome-scale mRNA transcriptomic insights into the mature growth dynamic of Cigar tobacco leaves

Yang

Wan

Qiao

et al. 2022

Preprint

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The harvest time is a key factor for cigar leaves with high quality, which varies greatly depending on the environment. Here, we performed a genome-scale mRNA transcriptomic analysis on the cigar cultivar CX-26 (Nicotiana tabacum L.) to evaluate the relationship between gene expression and growth state. The leaves were harvested with 67 (T1), 71(T2) and 75 (T3)-day growth. A total of 80,502 genes were detected in the CX-26 leaves, of which 64,611 genes were annotated on the reference genome. Principal component analysis showed that T1 and T2 leaves had a high overlapping pattern, while T3 leaves were distinct. Indeed, T1 and T2 leaves had fewer differential expressing genes (DEGs), while T3 leaves had 26,456 DGEs from T2 leaves, supporting the distinct growth of T3 leaves. GO annotations mainly enriched the photosynthesis-related metabolic process, catalytic activity and binding biological processes. KEEG analysis identified the key pathways including photosynthesis-antenna proteins, plant hormone/MAPK signaling pathway and plant-pathogen interaction. The maturity regulation and defense response-associated hormones abscisic acid and jasmonate acid were higher in the T3 leaves than that in T1 and T2 leaves confirming the KEEG analysis. Furthermore, several photosynthesis-related enzymes and a transcription factor were highlighted in the gene regulatory network, which might regulate the dynamics of carbohydrate metabolism, lipid metabolism and energy metabolism. In summary, our study provides insight into the growth state of CX-26 cigar leaves.

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Section: Keeg Analysis and Gene Regulatory Network Analysis Revealsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Genome-scale mRNA transcriptomic insights into the mature growth dynamic of Cigar tobacco leaves

Yang

Wan

Qiao

et al. 2022

Preprint

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“…47 Light-harvesting proteins are pigment-binding proteins containing Chls, carotenoids, and xanthophylls to harvest solar energy efficiently. 48 CP26, as a minor antenna, was located between the LHCII timers and PSII core, which was responsible for transferring the electronic excitation energy to the core. 49 In CP26, the crosslinked sites K58 and K92 were close to chlorophyll B601 with the binding sites of L59-P62 and chlorophyll A602 with the binding sites of L90-G91.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Crosslinker Nanocarriers Delivery to Chloroplasts for In Vivo Mapping of Photosynthetic Membrane Protein Complexes in Living Chlamydomonas reinhardtii Cells

Chen

Jiang

et al. 2023

Anal. Chem.

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Photosynthesis, as the core of solar energy biotransformation, is driven by photosynthetic membrane protein complexes in plants and algae. Current methods for intracellular photosynthetic membrane protein complex analysis mostly require the separation of specific chloroplasts or the change of the intracellular environment, which causes the missing of real-time and on-site information. Thus, we explored a method for in vivo crosslinking and mapping of photosynthetic membrane protein complexes in the chloroplasts of living Chlamydomonas reinhardtii (C. reinhardtii) cells under cultural conditions. Poly(lactic-co-glycolic acid) (PLGA) and poly(lactic-co-glycolic acid)-poly(ethylene glycol) (PLGA-PEG) nanoparticles were fabricated to deliver bis(succinimidyl)propargyl with a nitro compound (BSPNO) into the chloroplasts to crosslink photosynthetic membrane protein complexes. After the in vivo crosslinked protein complexes were extracted and digested, mass spectrometry was employed to detect lysine-specific crosslinked peptides for further elucidating the protein conformations and interactions. With this method, the weak interactions between extrinsic proteins in the luminal side (PsbL and PsbH) and the core subunits (CP47 and CP43) in photosynthetic protein complexes were directly captured in living cells. Additionally, the previously uncharacterized protein (Cre07.g335700) was bound to the light-harvesting proteins, which was related to the biosynthesis of light-harvesting antennae. These results indicated that in vivo analysis of photosynthetic protein complexes based on crosslinker nanocarriers was expected to not only figure out the difficulty in the study of photosynthetic protein complexes in living cells but also provide an approach to explore transient and weak interactions and the function of uncharacterized proteins.

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“…Oxygenic photosynthesis is responsible for the great biomass of cigar tobacco leaves. The photosynthesis antenna proteins function in light capturing and energy transferring by binding chlorophyll a, chlorophyll b, and carotenoids in the light‐harvesting chlorophyll protein complexes embedded in the thylakoid membrane in green plants (Arshad et al., 2022). At the same time, the gene expression between T2 and T3 leaves involved in carotenoid biosynthesis, porphyrin and chlorophyll metabolism, and carbon fixation in photosynthetic organisms was altered (Figure 7).…”

Section: Discussionmentioning

confidence: 99%

Genome‐scale transcriptomic insights into the mature growth dynamic of tobacco leaves

et al. 2023

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The harvest time is a key factor for cigar leaves with high quality, which varies greatly depending on the environment. In this study, we performed a growth characterization of the cigar tobacco CX-26 (Nicotiana tabacum L.) from the cluster stage to the vigorous stage and mature stage. Further, soil plant analysis development measurement of three middle leaves with 67 (T1), 71 (T2), and 75 days (T3) of growth after transplant indicates that the maturity might change from 71 to 75 days of growth. A genome-scale transcriptome was conducted to explore the gene expression dynamics of this maturity change. A total of 80,502 genes were detected in the CX-26 leaves, of which 64,611 genes were annotated on the reference genome. T1 and T2 leaves had fewer differential expressing genes (DEGs), while T3 leaves had 26,456 DEGs from T2 leaves, supporting the distinct growth of T3 leaves. Analyses of Gene ontology, Kyoto encyclopedia of genes and genomes identified that DEGs are involved inkey pathways including photosynthesis-antenna proteins, plant hormone/mitogenactivated protein kinase signaling pathway, and plant-pathogen interaction. Abscisic acid and jasmonate acids positively regulate leaf senescence and chlorophyll degradation and were higher in the T3 leaves compared with T1 and T2 leaves. In contrast, the total sugar was reduced in T3 leaves compared with T1 and T2 leaves, indicating the overripe state of the T3 leaves. Furthermore, several photosynthesis-related enzymes and a transcription factor were highlighted in the gene regulatory network, which might regulate the dynamics of carbohydrate metabolism, lipid metabolism, and energy metabolism. In summary, our study provides insight into the growth state of CX-26 cigar leaves.Abbreviations: ABA, abscisic acid; bZIP, basic leucine zipper; DEGs, differential expression genes; DHAR1, Dehdroascorbate reductase 1; GO, gene ontology; HSV, hue saturation value; JA, jasmonate acid; KEGG, Kyoto encyclopedia of genes and genomes; LHC, chlorophyll protein complex; MAPK, mitogen-activated protein kinase; PCA, principal component analysis; PHYA, Phytochrome A; SPA, suppressor of phyA-105 protein; SPAD, soil plant analysis development.

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A kaleidoscope of photosynthetic antenna proteins and their emerging roles

Cited by 16 publications

References 196 publications

Genome-scale mRNA transcriptomic insights into the mature growth dynamic of Cigar tobacco leaves

Genome-scale mRNA transcriptomic insights into the mature growth dynamic of Cigar tobacco leaves

Crosslinker Nanocarriers Delivery to Chloroplasts for In Vivo Mapping of Photosynthetic Membrane Protein Complexes in Living Chlamydomonas reinhardtii Cells

Genome‐scale transcriptomic insights into the mature growth dynamic of tobacco leaves

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