A JAK-STAT pathway target gene encoding a single WAP domain (SWD)-containing protein from Litopenaeus vannamei

A Novel Forkhead Box Protein P (FoxP) From Litopenaeus vannamei Plays a Positive Role in Immune Response

“…These indicated that the mechanism underlying hemocyte phagocytosis of V. parahaemolyticus could be different from those of other foreign particles, which requires further exploration. Previous studies have suggested that many secreted immune functional proteins, including these detected in this study, such as SWD4, SWD5, hemolin, and CTLs, could enhance hemocyte phagocytosis of bacteria in arthropods ( 25 , 33 , 39 , 40 ). The opsonization mechanisms mediated by these LvFoxP-regulated proteins could contribute to the stimulatory effect of LvFoxP on hemocyte phagocytosis.…”

“…Silencing of LvFoxP decreased expression of most immune effector proteins analyzed in this study, including CTLs, lysozyme, hemolin, PPOs, and AMPs from ALF, PEN, and Crustin families, which are all essential for humoral immune responses. Previous studies have described that PENs, ALFs and SWDs, and secreted immune functional proteins hemolin and CTLs possess both antibacterial and antiviral activities in shrimp ( 25 , 28 , 32 , 33 ). It can be inferred that through up-regulating expression of these proteins, LvFoxP may enhance immune responses in shrimp.…”

“…The coding sequence of LvFoxP was cloned into pAc5.1/V5-His A plasmid (Invitrogen, USA) to generate LvFoxP expression vector. The previously reported firefly luciferase plasmid PGL-Basic vectors (Promega, USA) containing promoters of antimicrobial peptides (AMPs), Dorsal, Relish, SWDs, CTL4 and lysozyme were used (25)(26)(27)(28)(29). Drosophila S2 cells were plated in 96-well plate and transfected with 0.05 mg firefly luciferase plasmid, 0.1 mg gene expression vector, and 0.03 mg pRL-TK renilla luciferase plasmid (as internal control) (Promega, USA).…”

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“…These indicated that the mechanism underlying hemocyte phagocytosis of V. parahaemolyticus could be different from those of other foreign particles, which requires further exploration. Previous studies have suggested that many secreted immune functional proteins, including these detected in this study, such as SWD4, SWD5, hemolin, and CTLs, could enhance hemocyte phagocytosis of bacteria in arthropods (25,33,39,40). The opsonization mechanisms mediated by these LvFoxPregulated proteins could contribute to the stimulatory effect of LvFoxP on hemocyte phagocytosis.…”

“…Silencing of LvFoxP decreased expression of most immune effector proteins analyzed in this study, including CTLs, lysozyme, hemolin, PPOs, and AMPs from ALF, PEN, and Crustin families, which are all essential for humoral immune responses. Previous studies have described that PENs, ALFs and SWDs, and secreted immune functional proteins hemolin and CTLs possess both antibacterial and antiviral activities in shrimp (25,28,32,33). It can be inferred that through up-regulating expression of these proteins, LvFoxP may enhance immune responses in shrimp.…”