A <i>Parachlorella kessleri</i> (Trebouxiophyceae, Chlorophyta) strain from an extremely acidic geothermal pond in Argentina

Juárez, Ángela Beatriz; Vélez, Carlos G.; Iñiguez, Abril R.; Martínez, Daniel E.; Rodríguez, María Cecilia; Vigna, María Susana; Molina, Marı́a del Carmen

doi:10.2216/10-79.1

Cited by 28 publications

(16 citation statements)

References 46 publications

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“…Besides the study of Novis (2006) for Klebsormidium acidophilum, results available for coccoid trebouxiophytes (Huss et al 2002, Juárez et al 2011 suggest that adaptation to low pH has taken place independently in different lineages and that in green microalgae acidophilic forms coexist with closely related neutrophilic forms. Here we investigate the phylogenetic relationships of low pH Klebsormidium using rbcL and ITS sequences of several strains isolated from acidic soils and rivers in Europe and U.S.A.…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence for the polyphyletic origin of low pH adaptation in the genus Klebsormidium (Klebsormidiophyceae, Streptophyta)

Škaloud¹,

Lukešová²,

Malavasi³

et al. 2014

Plecevo

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Background and aims -Algae living in low pH environments have been the subject of numerous studies, but their phylogenetic relationships with relatives found in non-acidic habitats are poorly known. In the present study we analyzed the morphology and phylogeny of acid-adapted strains of Klebsormidium, a genus of filamentous green algae frequently present in low pH environments. Methods -Eighteen strains of Klebsormidium were collected from from low pH habitats in Europe and U.S.A., mainly from terrestrial sites affected directly or indirectly by carbon mining activities. These algae were isolated in culture and their phylogenetic relationships were studied using rbcL and ITS rDNA sequence data in a concatenated dataset.

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Section: Introductionmentioning

confidence: 99%

Molecular evidence for the polyphyletic origin of low pH adaptation in the genus Klebsormidium (Klebsormidiophyceae, Streptophyta)

Škaloud¹,

Lukešová²,

Malavasi³

et al. 2014

Plecevo

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“…Parachlorella kessleri cells are characterised by a thicker (54-59 nm) cell wall than that in other 'Chlorella' species (Yamamoto et al 2005). Juárez and Vélez (2011) reported that the thickness of the cell wall could reach up 60 to 80 nm. Additionally, the fibrillar cell wall component of P. kessleri cells was composed of 100% of N-acetylglucosamine (chitin) (Takeda 1991;Juárez and Vélez 2011).…”

Section: Discussionmentioning

confidence: 98%

“…Juárez and Vélez (2011) reported that the thickness of the cell wall could reach up 60 to 80 nm. Additionally, the fibrillar cell wall component of P. kessleri cells was composed of 100% of N-acetylglucosamine (chitin) (Takeda 1991;Juárez and Vélez 2011). In the un-sonicated P. kessleri samples, the increase in the cell size with the time of cultivation was a result of the growth process, during which the volume of a particular cell and, in consequence, its diameter became bigger.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods

et al. 2018

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The main objective of this study is to demonstrate the possibilities of using laser light scattering methods, dynamic light scattering and laser Doppler electrophoresis, as suitable methods in investigations of algal production biosystems and biotechnology. This paper highlights the innovative use of the dynamic light scattering (DLS) methods for monitoring the destruction of Parachlorella kessleri cells. Additionally, these results indicate electrophoretic mobility as a new parameter to investigate the effectiveness of cell disruption prior to extraction conducted to optimise the biotechnological processes of recovery of microalgal intracellular metabolites. The efficacy of P. kessleri cell disintegration by ultrasound was determined by measurements of the number of cells with the algal cell reduction (CR ns), relative mean hydrodynamic diameter (Rd t) and electrophoretic mobility after applying different lengths of ultrasound exposure to a cell suspension. It was found that stationary-phase cells were the most resistant to the ultrasound treatment, especially at low values of the optical density. Both the relative hydrodynamic diameter and the electrophoretic mobility of cells were correlated statistically significantly with the time of sonication (t) and the algal cell reduction. The relationships allowed estimation of the sonication time needed for total cell disruption.

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“…Trebouxiophyceae are a ubiquitous class of photosynthetic unicellular Chlorophyta (=‘green algae’) encountered in many environments, including extreme ones such as hot (Büdel et al ., ; Flechtner et al ., ; Fučíková et al ., ) and cold (Cavacini, ; Fermani et al ., ; Hodač et al ., ) deserts, or hyper acidic waters (Juárez et al ., ). They are also common in mesophilic environments like soil and continental waters, some of them can also be found in the sea (Heesch et al ., ; Tragin and Vaulot, ).…”

Section: Introductionmentioning

confidence: 97%

Global distribution of Trebouxiophyceae diversity explored by high‐throughput sequencing and phylogenetic approaches

Metz

Domaizon

Unrein

et al. 2019

Environmental Microbiology

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Summary Trebouxiophyceae are a ubiquitous class of Chlorophyta encountered in aquatic and terrestrial environments. Most taxa are photosynthetic, and many acts as photobionts in symbiotic relationships, while others are free‐living. Trebouxiophyceae have also been widely investigated for their use for biotechnological applications. In this work, we aimed at obtaining a comprehensive image of their diversity by compiling the information of 435 freshwater, soil and marine environmental DNA samples surveyed with Illumina sequencing technology in order to search for the most relevant environments for bioprospecting. Freshwater and soil were most diverse and shared more than half of all operational taxonomic units (OTUs), however, their communities were significantly distinct. Oceans hosted the highest genetic novelty, and did not share any OTUs with the other environments; also, marine samples host more diversity in warm waters. Symbiotic genera usually found in lichens such as Trebouxia, Myrmecia and Symbiochloris were also abundantly detected in the ocean, suggesting either free‐living lifestyles or unknown symbiotic relationships with marine planktonic organisms. Altogether, our study opens the way to new prospection for trebouxiophycean strains, especially in understudied environments like the ocean.

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A Parachlorella kessleri (Trebouxiophyceae, Chlorophyta) strain from an extremely acidic geothermal pond in Argentina

Cited by 28 publications

References 46 publications

Molecular evidence for the polyphyletic origin of low pH adaptation in the genus Klebsormidium (Klebsormidiophyceae, Streptophyta)

Molecular evidence for the polyphyletic origin of low pH adaptation in the genus Klebsormidium (Klebsormidiophyceae, Streptophyta)

Effectiveness of Parachlorella kessleri cell disruption evaluated with the use of laser light scattering methods

Global distribution of Trebouxiophyceae diversity explored by high‐throughput sequencing and phylogenetic approaches

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