A
            <i>Narnavirus</i>
            in the Trypanosomatid Protist Plant Pathogen
            <i>Phytomonas serpens</i>

Akopyants, Natalia S.; Lye, Lon-Fye; Dobson, Deborah E.; Lukeš, J; Beverley, Stephen M.

doi:10.1128/genomea.00711-16

Cited by 22 publications

(18 citation statements)

References 8 publications

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“…In Leptomonas seymouri and two isolates of Phytomonas serpens we documented the presence of dsRNA (fragments of 2.9 + 1.5 kb and 3.8 kb, respectively) ( Fig. 4 A and B and Table 1), in agreement with previous findings (38)(39)(40) that these species bear Leptomonas seymouri narna-like virus 1 (LepseyNLV1) and Phytomonas serpens narnavirus 1 (PserNV1). Sequence features of trypanosomatid narnaviruses.…”

Section: Phylogenetic Analysis Of Lbv1s Suggests Classification As a supporting

confidence: 92%

“…Recently, molecular descriptions have been made for the viruses from several additional trypanosomatid species. Among them were a bunyavirus-like virus of Leptomonas moramango (37) as well as narnavirus-like viruses of Leptomonas seymouri (38) and the dixenous plant pathogen Phytomonas serpens (39). Provocatively, Leptomonas seymouri has been recovered from cultures from visceral leishmaniasis patients infected with Leishmania donovani, and many of such Leptomonas seymouri strains bear NLV1 (40).…”

Section: Significancementioning

confidence: 99%

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Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Grybchuk

Akopyants

Kostygov

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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125

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Knowledge of viral diversity is expanding greatly, but many lineages remain underexplored. We surveyed RNA viruses in 52 cultured monoxenous relatives of the human parasite ( and ), as well as plant-infecting was a hotbed for viral discovery, carrying a virus (Leptomonas pyrrhocoris ostravirus 1) with a highly divergent RNA-dependent RNA polymerase missed by conventional BLAST searches, an emergent clade of tombus-like viruses, and an example of viral endogenization. A deep-branching clade of trypanosomatid narnaviruses was found, notable as bearing Narna-like virus 1 (LepseyNLV1) have been reported in cultures recovered from patients with visceral leishmaniasis. A deep-branching trypanosomatid viral lineage showing strong affinities to bunyaviruses was termed "" (LBV) and judged sufficiently distinct to warrant assignment within a proposed family termed "" Numerous relatives of trypanosomatid viruses were found in insect metatranscriptomic surveys, which likely arise from trypanosomatid microbiota. Despite extensive sampling we found no relatives of the totivirus (LRV1/2), implying that it was acquired at about the same time the became able to parasitize vertebrates. As viruses were found in over a quarter of isolates tested, many more are likely to be found in the >600 unsurveyed trypanosomatid species. Viral loss was occasionally observed in culture, providing potentially isogenic virus-free lines enabling studies probing the biological role of trypanosomatid viruses. These data shed important insights on the emergence of viruses within an important trypanosomatid clade relevant to human disease.

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Section: Phylogenetic Analysis Of Lbv1s Suggests Classification As a supporting

confidence: 92%

Section: Significancementioning

confidence: 99%

Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Grybchuk

Akopyants

Kostygov

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

125

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“…Antarctic penguins have been 382 sampled since the 1970's, and avian avulaviruses have repeatedly been detected, both by 383 serology and PCR. The detection of phylogenetically related avian avulavirus 17 in 2013 in384 Adelie penguins (21), in2014-2016, and again in 2016 in Adelie 385 penguins as shown here, strongly suggests that these animals are an important reservoir for 386 these viruses. Although the influenza A virus we detected was the same virus as described 387 previously (15), the long branch lengths in the phylogenetic trees suggest long-term 388 undetected circulation in Antarctica (15).389 14The second key observation that indicates that penguins are potential virus reservoirs was the 390 presence of likely arboviruses, which is why we paired our analysis of the penguin virome 391 with that of the ticks that parasitise them.…”

mentioning

confidence: 54%

Sustained virome diversity in Antarctic penguins and their ticks: geographical connectedness and no evidence for low pathogen pressure

Wille

Harvey

Shī

et al. 2019

Preprint

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“…Due to the nature of the cloacal swabs, it is impossible to accurately determine the host for these viruses, although some information can be gleaned from the families in which these virus fall. For example, the Narnaviridae are known to infect fungi and protists, while the related Leviviridae infect bacteria [58][59][60][61]. Other novel viruses fell within invertebrate associated clades of the Nodaviridae and Tombusviridae, associated with both vertebrate and invertebrate infecting viruses in the Picornavirales [36].…”

Section: Discussionmentioning

confidence: 99%

Sustained RNA virome diversity in Antarctic penguins and their ticks

et al. 2020

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Despite its isolation and extreme climate, Antarctica is home to diverse fauna and associated microorganisms. It has been proposed that the most iconic Antarctic animal, the penguin, experiences low pathogen pressure, accounting for their disease susceptibility in foreign environments. There is, however, a limited understanding of virome diversity in Antarctic species, the extent of in situ virus evolution, or how it relates to that in other geographic regions. To assess whether penguins have limited microbial diversity we determined the RNA viromes of three species of penguins and their ticks sampled on the Antarctic peninsula. Using total RNA sequencing we identified 107 viral species, comprising likely penguin associated viruses (n = 13), penguin diet and microbiome associated viruses (n = 82), and tick viruses (n = 8), two of which may have the potential to infect penguins. Notably, the level of virome diversity revealed in penguins is comparable to that seen in Australian waterbirds, including many of the same viral families. These data run counter to the idea that penguins are subject to lower pathogen pressure. The repeated detection of specific viruses in Antarctic penguins also suggests that rather than being simply spill-over hosts, these animals may act as key virus reservoirs.

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A Narnavirus in the Trypanosomatid Protist Plant Pathogen Phytomonas serpens

Cited by 22 publications

References 8 publications

Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Viral discovery and diversity in trypanosomatid protozoa with a focus on relatives of the human parasite Leishmania

Sustained virome diversity in Antarctic penguins and their ticks: geographical connectedness and no evidence for low pathogen pressure

Sustained RNA virome diversity in Antarctic penguins and their ticks

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