Amus-51RIP allele for transformation ofNeurospora crassa

Abstract

This report describes the construction and characterization ofmus-51RIP70, an allele for high-efficiency targeted integration of transgenes into the genome of the model eukaryoteNeurospora crassa. Two of themus-51RIP70strains investigated in this work (RZS27.10 and RZS27.18) can be obtained from the Fungal Genetics Stock Center. The two deposited strains are, to our knowledge, genetically identical and neither one is preferred over the other for use inNeurosporaresearch.