A <i>Hoxa2</i> mutant conditional allele generated by Flp‐ and Cre‐mediated recombination

Ren, Shuyue; Pasqualetti, Massimo; Dierich, Andrée; Meur, Marianne Le; Rijli, Filippo M.

doi:10.1002/gene.10052

Cited by 19 publications

(31 citation statements)

References 15 publications

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“…We used a Wnt1::Cre transgenic mouse line in which the Wnt1 promoter drives Cre recombinase expression in NCC progenitors (Danielian et al, 1998) To assess the efficiency of the Wnt1::Cre mediated recombination, we carried out whole-mount in situ hybridization on E10.0 mutant embryos. We have previously shown that the Wnt1::Cre driver mated to a Hoxa2 floxed allele efficiently induces lack of Hoxa2 expression in NCCs (Ren et al, 2002;Santagati et al, 2005). Similarly, we tested the expression of Hoxa3 in Wnt1::Cre/ Hoxa flox/del mutant embryos and observed, as expected, a selective loss of Hoxa3 transcripts from the NCCs contributing to the third and more posterior PAs, whereas the other sites of Hoxa3 expression were unaffected (see Fig.…”

Section: Generation Of Hoxa and Hoxd Cluster Deletionssupporting

confidence: 66%

“…Generation of conventional Hoxa2 and conditional Hoxa2 flox mutant alleles has been described previously (Rijli et al, 1993;Ren et al, 2002). Mice mutant for the Hoxd cluster were as reported previously (Zakany et al, 2001).…”

Section: Stocks Of Mice and Matingsmentioning

confidence: 99%

“…The following RNA probes were used: Hoxa2 (Ren et al, 2002), Hoxb2 (Vesque et al, 1996), Hoxa3, Hoxb3, Hoxd3 (Manley and Capecchi, 1997), Hoxa4, Hoxb4, Hoxd4 (Gaunt et al, 1989), CRABP1 (Maden et al, 1992), Pax1 (Wallin et al, 1996), Alx4 (Qu et al, 1997), Pitx1 (Lanctot et al, 1999) and Barx1 (Tissier-Seta et al, 1995). Z/AP staining E15.5 embryos were fixed overnight in 4% paraformaldehyde (PFA), rinsed in phosphate-buffered saline (PBS), equilibrated in 20% sucrose and included in Cryomatrix (Thermo Electron Corporation).…”

Section: In Situ Hybridizationmentioning

confidence: 99%

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Rostral and caudal pharyngeal arches share a common neural crest ground pattern

et al. 2009

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In vertebrates, face and throat structures, such as jaw, hyoid and thyroid cartilages develop from a rostrocaudal metameric series of pharyngeal arches, colonized by cranial neural crest cells (NCCs). Colinear Hox gene expression patterns underlie arch specific morphologies, with the exception of the first (mandibular) arch, which is devoid of any Hox gene activity. We have previously shown that the first and second (hyoid) arches share a common, Hox-free, patterning program. However, whether or not more posterior pharyngeal arch neural crest derivatives are also patterned on the top of the same ground-state remained an unanswered question. Here, we show that the simultaneous inactivation of all Hoxa cluster genes in NCCs leads to multiple jaw and first archlike structures, partially replacing second, third and fourth arch derivatives, suggesting that rostral and caudal arches share the same mandibular arch-like ground patterning program. The additional inactivation of the Hoxd cluster did not significantly enhance such a homeotic phenotype, thus indicating a preponderant role of Hoxa genes in patterning skeletogenic NCCs. Moreover, we found that Hoxa2 and Hoxa3 act synergistically to pattern third and fourth arch derivatives. These results provide insights into how facial and throat structures are assembled during development, and have implications for the evolution of the pharyngeal region of the vertebrate head.

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Section: Generation Of Hoxa and Hoxd Cluster Deletionssupporting

confidence: 66%

Section: Stocks Of Mice and Matingsmentioning

confidence: 99%

Section: In Situ Hybridizationmentioning

confidence: 99%

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Rostral and caudal pharyngeal arches share a common neural crest ground pattern

et al. 2009

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“…The reporter gene expression patterns that are generated using the Hoxa2-associated enhancer elements define striped patterns in the hindbrain that resemble the original rhombomeres Ren et al, 2002) (data not shown). Because rhombomere development involves dynamic interactions among Hox and other genes (for review, see Glover et al, 2006), and because cell movement across rhombomere boundaries is known to occur (Birgbauer and Fraser, 1994;Wingate and Lumsden, 1996), an important question is whether the striped Hox gene expression patterns faithfully represent the rhombomere-derived domains throughout development.…”

Section: Fidelity Of Rhombomeric Domains Revealed By Hoxa2 Enhancer Ementioning

confidence: 90%

Fate-Mapping the Mammalian Hindbrain: Segmental Origins of Vestibular Projection Neurons Assessed Using Rhombomere-SpecificHoxa2Enhancer Elements in the Mouse Embryo

Pasqualetti¹,

Dı́az²,

Renaud³

et al. 2007

J. Neurosci.

121

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As a step toward generating a fate map of identified neuron populations in the mammalian hindbrain, we assessed the contributions of individual rhombomeres to the vestibular nuclear complex, a major sensorimotor area that spans the entire rhombencephalon. Transgenic mice harboring either the lacZ or the enhanced green fluorescent protein reporter genes under the transcriptional control of rhombomere-specific Hoxa2 enhancer elements were used to visualize rhombomere-derived domains. We labeled functionally identifiable vestibular projection neuron groups retrogradely with conjugated dextran-amines at successive embryonic stages and obtained developmental fate maps through direct comparison with the rhombomere-derived domains in the same embryos. The fate maps show that each vestibular neuron group derives from a unique rostrocaudal domain that is relatively stable developmentally, suggesting that anteroposterior migration is not a major contributor to the rostrocaudal patterning of the vestibular system. Most of the groups are multisegmental in origin, and each rhombomere is fated to give rise to two or more vestibular projection neuron types, in a complex pattern that is not segmentally iterated. Comparison with studies in the chicken embryo shows that the rostrocaudal patterning of identified vestibular projection neuron groups is generally well conserved between avians and mammalians but that significant speciesspecific differences exist in the rostrocaudal limits of particular groups. This mammalian hindbrain fate map can be used as the basis for targeting genetic manipulation to specific subpopulations of vestibular projection neurons.

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“…In Hoxa2 flox/flox ;Wnt1-Cre 10.5 day post coitum (dpc) embryos, Hoxa2 expression was selectively abolished from the NCCs contributing to the second and more posterior arches, as assessed by whole-mount in situ hybridisation ( Fig. 1C) (see also Ren et al, 2002). At birth, Hoxa2 flox/flox ;Wnt1-Cre mutant mice displayed a similar external phenotype to the conventional Hoxa2 -/-mutants (Rijli et al, 1993), namely the loss of external ear (Fig.…”

Section: Selective Deletion Of Hoxa2 In Second Arch Neural Crest Cellmentioning

confidence: 98%

Temporal requirement ofHoxa2in cranial neural crest skeletal morphogenesis

et al. 2005

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NCCs retain a remarkable degree of plasticity even after their migration in the arch, and require Hoxa2 as an integral component of their morphogenetic program. Moreover, subpopulations of postmigratory NCCs required Hoxa2 at discrete time points to pattern distinct derivatives. This study provides the first temporal inactivation of a vertebrate Hox gene and illustrates Hox requirement during late morphogenetic processes.

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A Hoxa2 mutant conditional allele generated by Flp‐ and Cre‐mediated recombination

Cited by 19 publications

References 15 publications

Rostral and caudal pharyngeal arches share a common neural crest ground pattern

Rostral and caudal pharyngeal arches share a common neural crest ground pattern

Fate-Mapping the Mammalian Hindbrain: Segmental Origins of Vestibular Projection Neurons Assessed Using Rhombomere-SpecificHoxa2Enhancer Elements in the Mouse Embryo

Temporal requirement ofHoxa2in cranial neural crest skeletal morphogenesis

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