A <i>Ganoderma atrum</i> polysaccharide alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier

Zheng, Bing; Ying, Mengxi; Xie, Mingyong; Chen, Yi; Wang, Yudan; Ding, Xiaomeng; Yu, Qiang

doi:10.1039/d0fo02260h

Cited by 40 publications

(37 citation statements)

References 36 publications

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“…The abnormal expression of TJs, including ZO-1 and occludin, allows harmful substances in the intestine to escape, which in turn causes intestinal inflammation. [9] MUC-2, an important component of the mucus component, is secreted by intestinal phagocytes and forms an essential chemical barrier against pathogens in the intestine. [20] MBP administration recovered the expression of MUC-2, and TJs in DSS-induced colitis mice (Figure 4), which is consistent with the results of Han et al [21] With the disruption of the intestinal barrier and progression of colitis, apoptosis is almost inevitable.…”

Section: Discussionmentioning

confidence: 99%

“…Animals were randomly and equally divided into six groups ( n = 8 per group): normal control group (NC); model control group (MC); 5‐ASA treatment group (ASA); low‐ (LD), medium‐ (MD), and high‐dose (HD) group. After the acclimation period, mice in the LD, MD, and HD groups were given MBP (100, 200, and 300 mg kg −1 d −1 ) [ 9 ] by oral gavage for 14 days, respectively. Meanwhile, the ASA group was given 5‐ASA (200 kg −1 d −1 ) at the same manner, the NC group was given saline of an equivalent volume.…”

Section: Methodsmentioning

confidence: 99%

“…Polysaccharides have shown great potential in the treatment of colitis. [9] As a traditional medicinal and edible plant, Mesona chinensis Benth (MBP) has detoxifying properties. Polysaccharides extracted from MBP have been shown to have a broad array of biological effects, such as diabetes alleviation, [10] cancer inhibition, [11] and immunomodulatory.…”

Section: Introductionmentioning

confidence: 99%

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Mesona chinensis Benth Polysaccharides Alleviate DSS‐Induced Ulcerative Colitis via Inhibiting of TLR4/MAPK/NF‐κB Signaling Pathways and Modulating Intestinal Microbiota

Shen

Chen

et al. 2022

Molecular Nutrition Food Res

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Scope Ulcerative colitis (UC) is a severe disease of the intestinal tract. To investigate the role of TLR4/Mitogen‐activated protein kinase (MAPK)/Nuclear factor kappa‐B(NF‐κB) pathways and intestinal flora in UC, and the protective mechanisms of Mesona chinensis Benth polysaccharides (MBP), potential therapeutic agents due to their diabetes‐relieving, cancer‐suppressing, and immunomodulatory properties. Methods and results A dextran sulfate sodium (DSS)‐induced mouse colitis model is used for experiments; the histopathology, immunohistochemistry, and Western blotting’s results suggest that MBP can alleviate the colitis symptoms, inhibits the overproduction of TNF‐α, IL‐1β, promote IL‐10, reduces myeloperoxidase activity, and alleviates the inflammatory response probably by inhibiting the activation of TLR4/MAPK/NF‐κB pathways. Furthermore, MBP improvs the ratio of Bcl‐2/BAX, maintains the intestinal integrity by promoting the levels of zonulin occludin‐1 (ZO‐1), occluding and mucin mucin‐2 (MUC‐2), reduces the levels of endotoxin (ET), lipopolysaccharide binding protein (LBP) in serum, and oxidative stress in liver. Moreover, using 16S rRNA Gene Sequencing analysis, MBP regulates gut microbiota by decreasing the abundances of Helicobacter and Prevotella and increasing the abundances of Lactobacillus and Coprococcus, reverses microbiota dysbiosis caused by DSS. Conclusion These findings confirm the anti‐inflammatory effects of MBP, restoration of the intestinal barrier and intestinal flora, and have therapeutic potential to attenuate the development of UC.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mesona chinensis Benth Polysaccharides Alleviate DSS‐Induced Ulcerative Colitis via Inhibiting of TLR4/MAPK/NF‐κB Signaling Pathways and Modulating Intestinal Microbiota

Shen

Chen

et al. 2022

Molecular Nutrition Food Res

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“…Importantly, diarrhea is the main factor of growth retardation in calves. The intestinal barrier function is closely associated with diarrhea [ 23 ]. The beneficial effects of NaH on the intestinal integrity have been found in broilers, possibly through a decreased intestinal permeability and an increased intestinal viscosity [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Sodium Humate on the Growth Performance, Diarrhea Incidence, Blood Parameters, and Fecal Microflora of Pre-Weaned Dairy Calves

Wang

You

et al. 2022

Animals

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This study aimed to evaluate the effects of the administration of sodium humate (NaH) on the growth performance, diarrhea incidence, and fecal microflora of pre-weaned Holstein calves. In a 53-day experiment, forty healthy newborn female calves were randomly allocated to the following four treatment groups: (1) control (basal diet); (2) 1-gram NaH (basal diet extra orally supplemented with 1 g of NaH dissolved in 100 mL of milk or milk replacer daily); (3) 3-gram NaH (basal diet extra orally supplemented with 3 g of NaH dissolved in 100 mL of milk or milk replacer daily); and (4) 5-gram NaH (basal diet extra orally supplemented with 5 g of NaH dissolved in 100 mL of milk or milk replacer daily). NaH was mixed with milk (d 2–20) or milk replacer (d 21–53). Calves in the 5-gram NaH group had a higher ADG during d 1 to 21 and d 21 to 53 than the other groups did (p < 0.05). Fecal scores and diarrheal incidence were significantly lower in the 3-gram and 5-gram NaH groups than the 1-gram NaH and control groups during d 1 to 20 (p < 0.05). The serum IgA, IgG and IL-4 concentrations, and T-SOD and T-AOC activities were higher, and the serum IL-6, TNF-α, D-lactic acid, and MDA concentrations were lower in the 5-gram NaH group than the control group (p < 0.05). Furthermore, NaH supplementation increased the abundances of Bifidobacterium and Lactobacillus but decreased the abundance of Escherichia coli in feces (p < 0.05). These encouraging findings indicated that supplementation with 5 g of NaH effectively improved the immune status, antioxidant capacity, and intestinal beneficial bacteria, and further improved the growth performance and reduced the diarrhea incidence of the pre-weaned dairy calves.

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“…Side chains were terminated by β-Glcp, with the composition of α-1,4-Galp, α-1,4-GalpA, β-1,3-Glcp, and β-1,6-Glcp [ 18 ]. PSG-1 have exhibited a broad range of beneficial health effects, including immunomodulatory, chemo-protective, antioxidant, and hypoglycemic activities [ 19 , 20 , 21 , 22 ]. In particular, latest studies have also proved that PSG-1 could modulate the mucosal immunity through epithelial cells [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Ganoderma atrum Polysaccharide on Acrolein-Induced Apoptosis and Autophagic Flux in IEC-6 Cells

Wang¹,

Chang²,

Zheng³

et al. 2022

Foods

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This study was designed to explore the beneficial effect and mechanism of Ganoderma atrum (G. atrum) polysaccharide (PSG-1) on acrolein-induced IEC-6 cells. Our results indicated that PSG-1 significantly reduced the impairment of acrolein on cell viability, decreased oxidative stress, and enabled normal expression of tight junction (TJ) proteins that were inhibited by acrolein in IEC-6 cells. Furthermore, PSG-1 attenuated the elevation of microtubule-associated proteins light chain 3 (LC3) and Beclin 1-like protein 1 (Beclin 1) and increased the protein levels of phospho-mTOR (p-mTOR) and phospho-akt (p-akt), indicating that PSG-1 activated the mammalian target of rapamycin (mTOR) signaling pathway and alleviated acrolein-induced autophagy in IEC-6 cells. Moreover, PSG-1 markedly attenuated the acrolein-induced apoptosis, as evidenced by the increase in mitochondrial membrane potential (MMP) and B-cell lymphoma 2 (Bcl-2) expression, and the decrease in cysteine aspartate lyase (caspase)-3 and caspase-9. In addition, autophagy the inhibitor inhibited acrolein-induced TJ and apoptosis of IEC-6 cells, while the apoptosis inhibitor also inhibited acrolein-induced TJ and autophagy, suggesting that autophagy and apoptosis were mutually regulated. Taken together, the present study proved that PSG-1 could protect IEC-6 cells from acrolein-induced oxidative stress and could repair TJ by inhibiting apoptosis and autophagic flux, where autophagy and apoptosis were mutually regulated.

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A Ganoderma atrum polysaccharide alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier

Abstract: DSS destroyed the intestinal mucosa, while a Ganoderma atrum polysaccharide repaired the intestinal epithelial physical barrier and the DC-related immune barrier.

Cited by 40 publications

References 36 publications

Mesona chinensis Benth Polysaccharides Alleviate DSS‐Induced Ulcerative Colitis via Inhibiting of TLR4/MAPK/NF‐κB Signaling Pathways and Modulating Intestinal Microbiota

Mesona chinensis Benth Polysaccharides Alleviate DSS‐Induced Ulcerative Colitis via Inhibiting of TLR4/MAPK/NF‐κB Signaling Pathways and Modulating Intestinal Microbiota

Influence of Sodium Humate on the Growth Performance, Diarrhea Incidence, Blood Parameters, and Fecal Microflora of Pre-Weaned Dairy Calves

Protective Effect of Ganoderma atrum Polysaccharide on Acrolein-Induced Apoptosis and Autophagic Flux in IEC-6 Cells

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