A <i>CsTu</i>‐<i><scp>TS</scp>1</i> regulatory module promotes fruit tubercule formation in cucumber

Yang, Sen; Wen, Changlong; Liu, Bin; Cai, Yanling; Xue, Shudan; Bartholomew, Ezra S.; Dong, Mingming; Chen, Jian; Xu, Shuo; Wang, Ting; Qi, Wenzhu; Pang, Jing; Mao, Dehua; Liu, Xingwang; Ren, Huazhong

doi:10.1111/pbi.12977

Cited by 30 publications

(29 citation statements)

References 48 publications

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“…1 a4), trichome and tubercule presence vs trichome and tubercule absence 25,26 (Fig. 1 a5–6), large vs small tubercules 27 (Fig. 1 a7, a7-B a7-S), and hard vs soft spines 28 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Selection footprints reflect genomic changes associated with breeding efforts in 56 cucumber inbred lines

et al. 2019

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Cucumber selective breeding over recent decades has dramatically increased productivity and quality, but the genomic characterizations and changes associated with this breeding history remain unclear. Here, we analyzed the genome resequencing data of 56 artificially selected cucumber inbred lines that exhibit various phenotypes to detect trait-associated sequence variations that reflect breeding improvement. We found that the 56 cucumber lines could be assigned to group 1 and group 2, and the two groups formed a distinctive genetic structure due to the breeding history involving hybridization and selection. Differentially selected regions were identified between group 1 and group 2, with implications for genomic-selection breeding signatures. These regions included known quantitative trait loci or genes that were reported to be associated with agronomic traits. Our results advance knowledge of cucumber genomics, and the 56 selected inbred lines could be good germplasm resources for breeding.

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“…1 a4), trichome and tubercule presence vs trichome and tubercule absence 25,26 (Fig. 1 a5–6), large vs small tubercules 27 (Fig. 1 a7, a7-B a7-S), and hard vs soft spines 28 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Selection footprints reflect genomic changes associated with breeding efforts in 56 cucumber inbred lines

et al. 2019

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“…It was worth noting that trichome type had distinct relationship with the existence of tubercules. Tubercules are derived from several layers of cells that lie near the trichome base with an expanded structure (Yang et al 2014(Yang et al , 2018 (Fig. S2).…”

Section: Morphological Classification Of Cucumber Fruit Trichomesmentioning

confidence: 99%

Classification of fruit trichomes in cucumber and effects of plant hormones on type II fruit trichome development

Xue

Dong

Liu

et al. 2018

Planta

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Cucumber fruit trichomes could be classified into eight types; all of them are multicellular with complex and different developmental processes as compared with unicellular trichomes in other plants. The fruit trichomes or fruit spines of cucumber, Cucumis sativus L., are highly specialized structures originating from epidermal cells with diverse morphology, which grow perpendicular to the fruit surface. To understand the underlying molecular mechanisms of fruit trichome development, in this study, we conducted morphological characterization and classification of cucumber fruit trichomes and their developmental processes. We examined the fruit trichomes among 200 cucumber varieties, which could be classified into eight morphologically distinct types (I-VIII). Investigation of the organogenesis of the eight types of trichomes revealed two main developmental patterns. The development of glandular trichomes had multiple stages including initiation and expansion of the trichome precursor cell protuberating out of the epidermal surface, followed by periclinal bipartition to two cells (top and bottom) which later formed the head region and the stalk, respectively, through subsequent cell divisions. The non-glandular trichome development started with the expansion of the precursor cell perpendicularly to the epidermal plane followed by cell periclinal division to form a stalk comprising of some rectangle cells and a pointed apex cell. The base cell then started anticlinal bipartition to two cells, which then underwent many cell divisions to form a multicellular spherical structure. In addition, phytohormones as environmental cues were closely related to trichome development. We found that GA and BAP were capable of increasing trichome number per fruit with distinct effects under different concentrations.

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“…This variation is likely due to multiple factors controlling epidermal cell shape, including the Pe gene on chromosome 5. Pe has been localized to a 0.23 Mb region and exhibits tight, but not unbreakable, linkage to several fruit surface related traits such as dull ( D ), uniform fruit color ( u ) and tuberculate ( Tu ), suggesting a cluster of genes modulating cucumber exocarp characteristics (Yang et al, 2014a, Yang et al, 2014b, Yang et al, 2014c; Chen et al, 2016; Zhang et al, 2017; Yang et al, 2019). The QTL for epidermal cell width identified on chromosome 5 in this study is consistent with the location of Pe .…”

Section: Discussionmentioning

confidence: 99%

QTL and Transcriptomic Analyses Implicate Cuticle Transcription Factor SHINE as a Source of Natural Variation for Epidermal Traits in Cucumber Fruit

et al. 2019

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The fruit surface is a unique tissue with multiple roles influencing fruit development, post-harvest storage and quality, and consumer acceptability. Serving as the first line of protection against herbivores, pathogens, and abiotic stress, the surface can vary markedly among species, cultivars within species, and developmental stage. In this study we explore developmental changes and natural variation of cucumber (Cucumis sativus L.) fruit surface properties using two cucumber lines which vary greatly for these traits and for which draft genomes and a single nucleotide polymorphism (SNP) array are available: Chinese fresh market type, Chinese Long ‘9930’ (CL9930), and pickling type, ‘Gy14’. Thin-section samples were prepared from the mid-region of fruit harvested at 0, 4, 8, 12, 16, 20, 24 and 30 days post pollination (dpp), stained with Sudan IV and evaluated for cuticle thickness, depth of wax intercalation between epidermal cells, epidermal cell size and shape, and number and size of lipid droplets. ‘Gy14’ is characterized by columnar shaped epidermal cells, a 2–3 fold thicker cuticular layer than CL9930, increased cuticular intercalations between cells and a larger number and larger sized lipid droplets. In both lines maximal deposition of cuticle and increase in epidermal size coincided with exponential fruit growth and was largely completed by approximately 16 dpp. Phenotyping and quantitative trait locus mapping (QTL) of fruit sampled from an F7:F8 Gy14 × CL9930 recombinant inbred line (RIL) population identified QTL regions on chromosomes 1, 4 and 5. Strong QTL for epidermal cell height, cuticle thickness, intercalation depth, and diameter of lipid droplets co-localized on chromosome 1. SSR markers on chromosome 1 were used to screen for recombinants in an extended RIL population to refine the QTL region. Further fine mapping by KASP assay combined with gene expression profiling suggested a small number of candidate genes. Tissue specificity, developmental analysis of expression, allelic diversity and gene function implicate the regulatory factor CsSHINE1/WIN1 as a source of natural variation for cucumber fruit epidermal traits.

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A CsTu‐TS1 regulatory module promotes fruit tubercule formation in cucumber

Cited by 30 publications

References 48 publications

Selection footprints reflect genomic changes associated with breeding efforts in 56 cucumber inbred lines

Selection footprints reflect genomic changes associated with breeding efforts in 56 cucumber inbred lines

Classification of fruit trichomes in cucumber and effects of plant hormones on type II fruit trichome development

QTL and Transcriptomic Analyses Implicate Cuticle Transcription Factor SHINE as a Source of Natural Variation for Epidermal Traits in Cucumber Fruit

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