A<i>Bemisia tabaci</i>midgut protein interacts with begomoviruses and plays a role in virus transmission

Rana, Vipin Singh; Popli, Sonam; Saurav, Gunjan Kumar; Raina, Harpreet Singh; Chaubey, Rahul; Ramamurthy, V. V.; Rajagopal, Raman

doi:10.1111/cmi.12538

Cited by 38 publications

(30 citation statements)

References 34 publications

(55 reference statements)

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“…The CP and AV2 proteins of ToLCNDV are involved in virus movement and their absence results in reduced levels of ssDNA (Padidam et al, 1996. The CP of ToLCNDV also interacts with a midgut protein of B. tabaci and probably facilitates virus transport both from the digestive tract to the haemolymph, and from the haemolymph to the salivary glands (Rana et al, 2016).…”

Section: Genome Organization and Proteinsmentioning

confidence: 99%

Tomato leaf curl New Delhi virus: a widespread bipartite begomovirus in the territory of monopartite begomoviruses

Zaidi

Martin

Amin

et al. 2016

Molecular Plant Pathology

114

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Section: Genome Organization and Proteinsmentioning

confidence: 99%

Tomato leaf curl New Delhi virus: a widespread bipartite begomovirus in the territory of monopartite begomoviruses

Zaidi

Martin

Amin

et al. 2016

Molecular Plant Pathology

114

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“…Similarly, a midgut protein of B . tabaci meditates entry of begomoviruses into the guts by interacting with CP of tomato leaf curl New Delhi virus and cotton leaf curl Rajasthan virus (Rana et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…In general, once orally acquired by its insect vector, a persistent circulative virus follows a sequential pathway, from ingestion through the stylet to gut to haemolymph and finally to the salivary glands (Jia et al, ; Liu et al, ). The successful spread of virions in the insect vectors requires specific interactions between the virus and vector components to overcome membrane barriers (Linz, Liu, Chougule, & Bonning, ; Rana et al, ) or prevent degradation by the vector's immune system (Gray et al, ; Liu et al, ; Xu et al, ). The virions are thought to hijack a host mechanism that most likely enables transport of essential macromolecules (Marsh & Helenius, ; Seddas et al, ; Wang, Wu, Liu, Wu, & Wang, ).…”

Section: Introductionmentioning

confidence: 99%

ADP ribosylation factor 1 facilitates spread of wheat dwarf virus in its insect vector

Wang

Liu

Zhang

et al. 2019

Cellular Microbiology

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Many plant viruses are vectored by insects in a persistent circulative manner. The insect gut and salivary gland are important barriers limiting virus spread, but the mechanisms by which viruses are able to cross the gut escape barriers of the insect remain largely unknown. Wheat dwarf virus (WDV), transmitted by Psammotettix alienus in a persistent, circulative, and nonpropagative manner, causes the most economically important virus disease in wheat. In this study, ADP ribosylation factor 1 (ARF1) was found to interact with the coat protein of WDV in a yeast two-hybrid, pull-down assay and to colocalise with virions in the gut and salivary glands of P. alienus. When transcription of ARF1 was suppressed by RNA interference, the WDV titre decreased in the haemolymph and salivary glands, and transmission efficiency decreased, but titre in the gut did not differ from that of the control. These data suggest that ARF1 of P. alienus binds to the WDV virion and helps virus spread from gut to haemolymph. Our study provides direct experimental evidence that WDV can use the existing membrane trafficking mechanism to aid its spread within the insect vector. This first analysis of the molecular interaction between WDV and its vector P. alienus contributes to understanding the mechanisms involved in circulative transmission of the virus by the leafhopper vector.

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“…For example, in the aphid Myzus persicae , the coat protein read-through domain (CP-RTD) of Beet western yellows virus binds Rack-1 and membrane-bound glyceraldehyde-3-phosphate dehydrogenase; these processes are thought to facilitate transcytosis of luteoviruses in the aphid midgut and accessory salivary glands [11]. The coat proteins of Tomato leaf curl New Delhi virus and Cotton leaf curl Rajasthan virus were shown to interact with Bemisia tabaci midgut protein to facilitate trafficking of viral particles from the midgut into the insect hemolymph [12]. Furthermore, the Rice ragged stunt virus nonstructural protein Pns10 interacted with the Nilaparvata lugens oligomycin-sensitivity conferral protein to enhance virus titer in salivary gland cells [13].…”

Section: Introductionmentioning

confidence: 99%

The α-tubulin of Laodelphax striatellus facilitates the passage of rice stripe virus (RSV) and enhances horizontal transmission

Chen

et al. 2018

Preprint

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14Rice stripe virus (RSV), causal agent of rice stripe disease, is transmitted by the small 15 brown planthopper (SBPH, Laodelphax striatellus) in a persistent manner. The midgut and 16 salivary glands of SBPH are the first and last barriers in viral circulation and transmission, 17 respectively; however, the precise mechanisms used by RSV to cross these organs and 18 re-inoculate rice have not been fully elucidated. We obtained full-length cDNA of L. 19 striatellus α-tubulin 2 (LsTUB) and found that RSV infection increased the level of LsTUB 20 in vivo. Furthermore, LsTUB was shown to bind the RSV nonstructural protein 3 (NS3) in 21 vitro. RNAi was used to reduce LsTUB expression, which caused a significant reduction in 22 RSV titer, NS3 expression, RSV inoculation rates, and transmission to healthy plants. 23 Electrical penetration graphs (EPG) showed that LsTUB knockdown by RNAi did not 24 impact SBPH feeding; therefore, the reduction in RSV inoculation rate was likely caused 25 by the decrease in RSV transmission. These findings suggest that LsTUB mediates the 26 passage of RSV through midgut and salivary glands and leads to successful horizontal 27 transmission. 30The survival of plant viruses is largely dependent on the efficient transmission to plant 31 hosts by viral-specific vector(s) [1,2]. Insects transmit over 70% of all known plant viruses 32 [3]. Hemipteran insects (e.g. leafhoppers, planthoppers, aphids and whiteflies), transmit 33 approximately 55% of insect-vectored plant viruses; as these insects have distinctive 34 piercing-sucking mouthparts with needle-like stylet bundles that are comprised of two 35 maxillary and two mandibular stylets [3-5]. Four categories of insect vector -plant virus 36 transmission relationships have been described as follows: non-persistent; semi-persistent; 37 persistent: propagative and persistent: circulative [2,6]. Plant viruses with persistent 38 relationships enter vectors via the gut, pass through various tissues and ultimately reach the 39 salivary glands and ovaries. Viruses are transmitted horizontally from the salivary glands 40 of the vector into healthy plants and are vertically transmitted from female ovaries to 41 offspring [7]. Barriers to the persistent transmission of plant viruses in insect vectors 42 include the following: (i) midgut infection barriers; (ii) dissemination barriers, including 43 midgut escape and salivary gland infection barriers; (iii) salivary gland escape barriers; and 44 (iv) transovarial transmission barriers [8,9]. A deeper understanding of the mechanistic 45 basis of virus transmission through these four barriers will facilitate the development of 46 novel methods to control the systemic spread of plant viruses [10]. 47 Previous studies demonstrated that the persistent transmission of viruses in 48 different insect tissues requires specialized interactions between components of the virus 49 and vector. For example, in the aphid Myzus persicae, the coat protein read-through 50 domain (CP-RTD) of Beet western yellows virus...

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ABemisia tabacimidgut protein interacts with begomoviruses and plays a role in virus transmission

Cited by 38 publications

References 34 publications

Tomato leaf curl New Delhi virus: a widespread bipartite begomovirus in the territory of monopartite begomoviruses

Tomato leaf curl New Delhi virus: a widespread bipartite begomovirus in the territory of monopartite begomoviruses

ADP ribosylation factor 1 facilitates spread of wheat dwarf virus in its insect vector

The α-tubulin of Laodelphax striatellus facilitates the passage of rice stripe virus (RSV) and enhances horizontal transmission

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