A Hydrophobic Dye‐Encapsulated Nano‐Hybrid as an Efficient Fluorescent Probe for Living Cell Imaging

Chang, Shiuh‐Young; Wu, Xumeng; Li, Yongsheng; Niu, Dechao; Ma, Zhi; Zhao, Wenru; Gu, Jinlou; Wu, Dong; Ding, Feng; Zhu, Weihong; Shi, Jianlin

doi:10.1002/adhm.201200144

Cited by 22 publications

(21 citation statements)

References 22 publications

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“…Nevertheless, the majority of dyes have hydrophobic characteristics, which greatly hinder their applications in vitro and in vivo . 24 The advancement in encapsulation technology allowed the development of various strategies to encapsulate hydrophobic dyes and easily use them for different biomedical applications. The concept of loading dyes in nanostructures is not recent.…”

Section: Resultsmentioning

confidence: 99%

Dye-Loaded Polymersome-Based Lateral Flow Assay: Rational Design of a COVID-19 Testing Platform by Repurposing SARS-CoV-2 Antibody Cocktail and Antigens Obtained from Positive Human Samples

et al. 2021

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The global pandemic of COVID-19 continues to be an important threat, especially with the fast transmission rate observed after the discovery of novel mutations. In this perspective, prompt diagnosis requires massive economical and human resources to mitigate the disease. The current study proposes a rational design of a colorimetric lateral flow immunoassay (LFA) based on the repurposing of human samples to produce COVID-19-specific antigens and antibodies in combination with a novel dye-loaded polymersome for naked-eye detection. A group of 121 human samples (61 serums and 60 nasal swabs) were obtained and analyzed by RT-PCR and ELISA. Pooled samples were used to purify antibodies using affinity chromatography, while antigens were purified via magnetic nanoparticles-based affinity. The purified proteins were confirmed for their specificity to COVID-19 via commercial LFA, ELISA, and electrochemical tests in addition to sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Polymersomes were prepared using methoxy polyethylene glycol- b -polycaprolactone (mPEG- b -PCL) diblock copolymers and loaded with a Coomassie Blue dye. The polymersomes were then functionalized with the purified antibodies and applied for the preparation of two types of LFA (antigen test and antibody test). Overall, the proposed diagnostic tests demonstrated 93 and 92.2% sensitivity for antigen and antibody tests, respectively. The repeatability (92–94%) and reproducibility (96–98%) of the tests highlight the potential of the proposed LFA. The LFA test was also analyzed for stability, and after 4 weeks, 91–97% correct diagnosis was observed. The current LFA platform is a valuable assay that has great economical and analytical potential for widespread applications.

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Section: Resultsmentioning

confidence: 99%

Dye-Loaded Polymersome-Based Lateral Flow Assay: Rational Design of a COVID-19 Testing Platform by Repurposing SARS-CoV-2 Antibody Cocktail and Antigens Obtained from Positive Human Samples

et al. 2021

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“…Fluorescent bioprobes can precisely extract disease‐related information from complex biological systems due to their high sensitivity and resolution, low cost, and real‐time monitoring capability without radioactivity . Organic dyes are widely exploited as fluorescent biomarkers for near‐infrared (NIR) imaging since they are richer in variety, and much more compatible with living cells with respect to quantum dots (QDs) . However, there still exist two major obstacles to realize efficient bioprobing with organic dyes: (i) the poor aqueous dispersibility and bioenvironmental stability; (ii) the emission quenching at high loading contents which is notoriously known as aggregation‐caused quenching (ACQ) effect .…”

Section: Methodsmentioning

confidence: 99%

Morphology‐Tailoring of a Red AIEgen from Microsized Rods to Nanospheres for Tumor‐Targeted Bioimaging

Shao

Wang

et al. 2016

Advanced Materials

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Efficient morphology modulation of a red AIEgen from pristine microsized rods to nanospheres is achieved via encapsula ting QM-2 (quinolinemalononitrile-2) into hybrid micelles. This novel reagent shows great potential in tumor-targeted bioimaging because of its monodispersion in aqueous systems, the uniform diameter of ≈30 nm, enhanced fluorescence brightness with a large Stokes shift of 190 nm, and strongly increased biocompatibility and photostability.

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“…Multicolor luminescence and even white light emission could be obtained by neutralizing the solution after a certain time. To address the issue of fluorescent molecules leaking from micelles in practical applications, [22] we designed a cross‐linked silica shell that encapsulates micelles through OTES (Octyltriethoxysilane) and APTES ((3‐Aminopropyl) triethoxysilane) silane coupling agents (Figure S19). Note that the alkaline hydrolysis of APTES provided sufficient OH − to affect luminescence (Figure 4 a, S20).…”

Section: Figurementioning

confidence: 99%

Spatial Confinement Tunes Cleavage and Re‐Formation of C=N Bonds in Fluorescent Molecules

et al. 2021

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Molecules in confined spaces exhibit unusual behaviors that are not typically observed in bulk systems. Such behavior can provide alternative strategies for exploring new reaction pathways. Cleavage of the C=N bond of Nile red (NR) in solution is an irreversible reaction. Here, we used spatial confinement within a cationic micelle‐confined system to convert this reaction to a reversible process. The fluorescence of NR shifted between red and green for nine cycles. The new chemical pathway based on spatial confinement can be attributed to two factors: increasing the local concentration of reactants and reducing the reaction energy barrier. This effect is supported by both experimental evidence and theoretical calculations. The cross‐linked silica shell comprising the confinement chamber stabilizes the enclosed molecules. This reduces fluorophore leakage and maintains fluorescence intensity in most environments, including in solution, on paper, and in hydrogel films, and expands practical applications in encrypted information and multi‐informational displays.

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A Hydrophobic Dye‐Encapsulated Nano‐Hybrid as an Efficient Fluorescent Probe for Living Cell Imaging

Cited by 22 publications

References 22 publications

Dye-Loaded Polymersome-Based Lateral Flow Assay: Rational Design of a COVID-19 Testing Platform by Repurposing SARS-CoV-2 Antibody Cocktail and Antigens Obtained from Positive Human Samples

Dye-Loaded Polymersome-Based Lateral Flow Assay: Rational Design of a COVID-19 Testing Platform by Repurposing SARS-CoV-2 Antibody Cocktail and Antigens Obtained from Positive Human Samples

Morphology‐Tailoring of a Red AIEgen from Microsized Rods to Nanospheres for Tumor‐Targeted Bioimaging

Spatial Confinement Tunes Cleavage and Re‐Formation of C=N Bonds in Fluorescent Molecules

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