A Hydrolysis Method Using Microwaves: Determination of N-Acetyl- and N-Glycolylneuraminic Acids in Biological Systems by Fluorometric High-Performance Liquid Chromatography

“…Several methods have been developed to quantify Neu5Ac in biological samples including Warren method spectrophotometry [3][4][5][6][7][8][9], spectrofluorimetry [10], enzymology [11], gas chromatography (GC) [12] and high-performance liquid chromatography (HPLC) coupled with ultraviolet [13,14], fluorimetric [15][16][17][18], amperometric [19], and mass spectrometry detection [20][21][22][23][24][25]. The limit of the classical Waren method is its lack of specificity.…”

Section: Introductionmentioning

confidence: 99%

Measurement of free and total sialic acid by isotopic dilution liquid chromatography tandem mass spectrometry method

Tebani

Schlemmer

Imbard

et al. 2011

Journal of Chromatography B

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“…In the laboratory, the microwave generator used early on was a laboratory-grade instrument; for example, in 1993, Lagana et al (32) described the analysis of sialic acids using the Microwave Digestion System (CEM Co.). More recently, household microwave ovens have been used for chemical analysis of protein, lipid, and sugar structures in the laboratory (33)(34)(35)(36)(37)(38).…”

Section: Discussionmentioning

confidence: 99%

Microwave-mediated analysis for sugar, fatty acid, and sphingoid compositions of glycosphingolipids

Itonori

Takahashi

Kitamura

et al. 2004

Journal of Lipid Research

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For chemical characterization of glycosphingolipids, it is necessary to determine the chemical compositions of three constituents, i.e., sugars, fatty acids, and sphingoids. A new rapid analytical method is described using a one-pot reaction in a household microwave oven, producing sugars, fatty acids, and especially sphingoids free of byproducts, from a single aliquot of a biological sample. Glycosphingolipids were hydrolyzed by microwave exposure with 0.1 M NaOH/CH 3 OH for 2 min followed by 1 M HCl/ CH 3 OH for 45 s. The alkaline methanolysis step produced intermediate lysoglycosphingolipids virtually free of byproducts such as the O -methyl ethers usually seen. The fatty acid methyl esters were extracted with n-hexane, and other reaction products were dried, taken up in aqueous alkaline methanol, and shaken with chloroform. Sphingoids partitioned into the organic phase under these conditions, whereas the sugar portion that partitioned into the aqueous phase was re-N -acetylated and remethanolyzed for 30 s by microwave exposure.Analysis of the profiles of glycosphingolipid constituents obtained using the microwave oven method showed that they were quantitatively and qualitatively comparable to those obtained by time-consuming conventional methods, which require reaction for several hours. Analysis of the three constituents, including analysis by gas chromatography, may be obtained within 1 day using the method described here.

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A Hydrolysis Method Using Microwaves: Determination of N-Acetyl- and N-Glycolylneuraminic Acids in Biological Systems by Fluorometric High-Performance Liquid Chromatography

Cited by 22 publications

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Quantitation of N-acetylneuraminic (sialic) acid in bovine glycomacropeptide (GMP)

Quantitation of N-acetylneuraminic (sialic) acid in bovine glycomacropeptide (GMP)

Measurement of free and total sialic acid by isotopic dilution liquid chromatography tandem mass spectrometry method

Microwave-mediated analysis for sugar, fatty acid, and sphingoid compositions of glycosphingolipids

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