A hydrogen peroxide biosensor based on nano-Au/PAMAM dendrimer/cystamine modified gold electrode

Liu, Zhimin; Yang, Yibin; Wang, Hua; Li, Yanli; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1016/j.snb.2004.08.023

Cited by 139 publications

(73 citation statements)

References 32 publications

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“…-CS/Pt electrodes [55], HRPPPy electrode [56], HRP-TiO 2 film electrodes [57], and HRP/CeO 2 /CHT modified GC electrode [27]. From the slope of the regression equation, the sensitivity of the biosensor estimated was 243.1 AE 17.2 mA mM [64,65]. The reason might be that ascorbic acid can reduce benzoquinone (the oxidation form of mediator), which destroyed the circulation of mediator.…”

Section: Amperometric Response and Enzymatic Kinetic Parametermentioning

confidence: 99%

A MgO Nanoparticles Composite Matrix‐Based Electrochemical Biosensor for Hydrogen Peroxide with High Sensitivity

Zhang

et al. 2010

Electroanalysis

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A novel horseradish peroxidase (HRP) electrochemical biosensor based on a MgO nanoparticles (nano-MgO)-chitosan (chit) composite matrix was developed. The morphology of nano-MgO-chit nanocomposite was examined by scanning electron microscopy (SEM). The interaction between nano-MgO-chit nanocomposite matrix and enzyme was characterized with UV-vis spectra. This proposed composite material combined the advantages of inorganic nanoparticles and organic polymer chit. The HRP immobilized in the nanocomposite matrix displayed excellent electrocatalytic activity to the reduction of H 2 O 2 in the presence of hydroquinone as a mediator. The effects of the experimental variables such as solution pH and the working potential were investigated using steady-state amperometry. The present biosensor (HRP-modified electrode) had a fast response towards H 2 O 2 (less than 10 s), and excellent linear relationships were obtained in the concentration range of 0.1 -1300 mM, with a detection limit of 0.05 mM (S/N ¼ 3). Moreover, the stability and reproducibility of this biosensor were evaluated with satisfactory results.

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Section: Amperometric Response and Enzymatic Kinetic Parametermentioning

confidence: 99%

A MgO Nanoparticles Composite Matrix‐Based Electrochemical Biosensor for Hydrogen Peroxide with High Sensitivity

Zhang

et al. 2010

Electroanalysis

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“…32 In terms of stabilization, the treatment of enzymes that previously adsorbed on dendrimers bearing primary amino groups with glutaraldehyde, in many cases offers very good results, because this technique permits the crosslink between glutaraldehyde molecules bound to the enzyme and glutaraldehyde molecules bound to the dendrimer. 33 The primary amino groups of both enzyme and the dendrimer possess on its surface are activated with the molecule of glutaraldehyde. 34 Hence, an intense crosslinking between dendrimer and enzyme seems to be achieved, and this multiple covalent links between the enzyme and dendrimer stabilize the quaternary structure of the protein, and also rigidity of the subunit structures is increased.…”

Section: Introductionmentioning

confidence: 99%

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Yüksel

Akin

Geyik

et al. 2011

Biotechnology Progress

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This article deals with the use of pyranose oxidase (PyOx) and glucose oxidase (GOx) enzymes in amperometric biosensor design and their application in monitoring fermentation processes with the combination of flow injection analysis (FIA). The amperometric studies were carried out at -0.7 V by following the oxygen consumption due to the enzymatic reactions for both batch and FIA modes. Optimization studies (enzyme amounts and pH) and analytical parameters such as linearity, repeatability, effect of interference, storage, and operational stabilities have been studied. Under optimized conditions, for the PyOx-based biosensor, linear graph was obtained from 0.025 to 0.5 mM glucose in phosphate buffer (50 mM) at pH 7.0 with the equation of y = 3.358x + 0.028 and R(2) = 0.998. Linearity was found to be 0.01-1.0 mM in citrate buffer (50 mM and pH 4.0) with the equation of y = 1.539x + 0.181 and R(2) = 0.992 for the GOx biosensor. Finally, these biosensor configurations were further evaluated in a conventional flow injection system. Results from batch experiments provide a guide to design sensitive, stable, and interference-free biosensors for FIA mode. Biosensor stability, dynamic range, and repeatability were also studied in FIA conditions, and the applicability for the determination of glucose in fermentation medium could be successfully demonstrated. The FIA-combined glucose biosensor was used for the offline monitoring of yeast fermentation. The obtained results correlated well with HPLC measurements.

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“…Among various dendrimers, poly(amidoamine) (PAMAM) is the most frequently studied. A H 2 O 2 biosensor based on nano-Au/PAMAM dendrimer/cystamine modified gold electrode exhibited high sensitivity and good stability and reproducibility [23]. Godinez modified gold bead electrode with PB containing starburst PAMAM dendrimer to afford mixed and stable electrocatalytic layers which not only showed an improved surface coverage of PB on the dendrimer modified electrode but also showed an enhanced stability at neutral pH values [24].…”

mentioning

confidence: 99%

Improvement of Selectivity and Stability of Amperometric Detection of Hydrogen Peroxide Using Prussian Blue‐PAMAM Supramolecular Complex Membrane as a Catalytic Layer

Wang

et al. 2007

Electroanalysis

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A novel hydrogen peroxide (H 2 O 2 ) sensor was fabricated by using a submonolayer of 3-mercaptopropionic acid (3-MPA) adsorbed on a polycrystalline gold electrode further reacted with poly(amidoamine) (PAMAM) dendrimer (generation 4.0) to obtain a film on which Prussian Blue (PB) was later coordinated to afford a mixed and stable electrocatalytic layer for H 2 O 2 reduction. On the basis of the electrochemical behaviors, atomic force microscopy (AFM) and X-ray photoelectron spectra (XPS), it is suggested that the PB molecules are located within the dendritic structure of the surface attached PAMAM dendrimers. It was found that the PB/PAMAM/3-MPA/Au modified electrode showed an excellent electrocatalytic activity for H 2 O 2 reduction. The effects of applied potential and pH of solution upon the response of the modified electrode were investigated for an optimum analytical performance. Even in the presence of dissolved oxygen, the sensor exhibited highly sensitive and rapid response to H 2 O 2 . The steady-state cathodic current responses of the modified electrode obtained at À 0.20 V (vs. SCE) in air-saturated 0.1 mol L À1 phosphate buffer solution (PBS, pH 6.50) showed a linear relationship to H 2 O 2 concentration ranging from 1.2 Â 10 À6 mol L À1 to 6.5 Â 10 À4 mol L À1 with a detection limit of 3.1 Â 10 À7 mol L À1. Performance of the electrode was evaluated with respected to possible interferences such as ascorbic acid and uric acid etc. The selectivity, stability, and reproducibility of the modified electrode were satisfactory.

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A hydrogen peroxide biosensor based on nano-Au/PAMAM dendrimer/cystamine modified gold electrode

Cited by 139 publications

References 32 publications

A MgO Nanoparticles Composite Matrix‐Based Electrochemical Biosensor for Hydrogen Peroxide with High Sensitivity

A MgO Nanoparticles Composite Matrix‐Based Electrochemical Biosensor for Hydrogen Peroxide with High Sensitivity

Offline glucose biomonitoring in yeast culture by polyamidoamine/ cysteamine‐modified gold electrodes

Improvement of Selectivity and Stability of Amperometric Detection of Hydrogen Peroxide Using Prussian Blue‐PAMAM Supramolecular Complex Membrane as a Catalytic Layer

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