A hybridisation technique to identify anthelmintic resistance genes in Haemonchus1Note: Nucleotide sequence data reported in this paper are available in the GenBank™ database under accession numbers AF182011–AF182013.1

Jambre, L.F. Le; Lenane, I.J.; Wardrop, Alison J.

doi:10.1016/s0020-7519(99)00157-5

Cited by 35 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mapping anthelmintic resistance using marker association has been a consistent topic at CARS meetings and has its origin with some seminal work on microsatellite markers in H. contortus , Teladorsagia circumcincta and Trichostrongylus colubriformis ( Hoekstra et al, 1997; Otsen et al, 2000; Grillo et al, 2006; Redman et al, 2008 ) and the use of a H. contortus / placei species-hybrid system ( Le Jambre et al, 1999 ). Though SNP markers and small indels may well replace the use of microsatellite markers in the near future, these markers are still useful and have been used to establish the methodology for mapping anthelmintic resistance in H. contortus ( Hunt et al, 2010; Redman et al, 2012 ).…”

Section: Macrocyclic Lactones: Genetic Marker Association Mappingmentioning

confidence: 99%

Recent advances in candidate-gene and whole-genome approaches to the discovery of anthelmintic resistance markers and the description of drug/receptor interactions

Kotze¹,

Hunt²,

Skuce³

et al. 2014

International Journal for Parasitology: Drugs and Drug Resistan

168

180

View full text Add to dashboard Cite

show abstract

Section: Macrocyclic Lactones: Genetic Marker Association Mappingmentioning

confidence: 99%

Recent advances in candidate-gene and whole-genome approaches to the discovery of anthelmintic resistance markers and the description of drug/receptor interactions

Kotze¹,

Hunt²,

Skuce³

et al. 2014

International Journal for Parasitology: Drugs and Drug Resistan

168

180

View full text Add to dashboard Cite

show abstract

“…Test PCR amplifications were carried out on the six candidate genes using primer pairs reported in previously published studies (Blackhall et al (1998a(Blackhall et al ( , 1998b(Blackhall et al ( , 2003, Jagannathan et al, 1999;Le Jambre et al, 1999;Urdaneta-Marquez et al, 2014). Only the Hco-lgc-37 primer pair from Blackhall et al (2003) amplified sufficiently robustly in all five populations and were used in this study.…”

Section: Pcr Amplificationmentioning

confidence: 99%

“…Hco-pgp-9 and Hcopgp-2 were originally designated as Hco-pgp-1 and Hco-pgp-A, respectively (Xu et al, 1998;Le Jambre et al, 1999), but were subsequently re-named based on the identification of their respective C. elegans orthologues (Williamson and Wolstenholme, 2012). This gene family, known to be involved in ivermectin efflux in mammals (Schinkel, 1999), has been suggested to be involved in ivermectin resistance in parasitic nematodes in a number of studies and reviews (Blackhall et al, 1998b;Xu et al, 1998;Le Jambre et al, 1999;Sangster et al, 1999;Bartley et al, 2009;Williamson and Wolstenholme, 2012). In the case of Hco-pgp-2, Blackhall et al (1998b) used the same methodology and strains as used for Hco-glc-5 and Hco-lgc-37.…”

Section: Introductionmentioning

confidence: 99%

Evidence from two independent backcross experiments supports genetic linkage of microsatellite Hcms8a20, but not other candidate loci, to a major ivermectin resistance locus in Haemonchus contortus

Rezansoff

Laing

Gilleard

2016

International Journal for Parasitology

View full text Add to dashboard Cite

Haemonchus contortus is the leading parasitic nematode species used to study anthelmintic drug resistance. A variety of candidate loci have been implicated as being associated with ivermectin resistance in this parasite but definitive evidence of their importance is still lacking. We have previously performed two independent serial backcross experiments to introgress ivermectin resistance loci from two H. contortus ivermectin-resistant strains - MHco4(WRS) and MHco10(CAVR) - into the genetic background of the ivermectin-susceptible genome reference strain MHco3(ISE). We have interrogated a number of candidate ivermectin resistance loci in the resulting backcross populations and assessed the evidence for their genetic linkage to an ivermectin resistance locus. These include the microsatellite marker Hcms8a20 and six candidate genes Hco-glc-5, Hco-avr-14, Hco-lgc-37 (previously designated Hco-hg-1), Hco-pgp-9 (previously designated Hco-pgp-1), Hco-pgp-2 and Hco-dyf-7. We have sampled the haplotype diversity of amplicon markers within, or adjacent to, each of these loci in the parental strains and fourth generation backcross populations to assess the evidence for haplotype introgression from the resistant parental strain into the genomic background of the susceptible parental strain in each backcross. The microsatellite Hcms8a20 locus showed strong evidence of such introgression in both independent backcrosses, suggesting it is linked to an important ivermectin resistance mutation in both the MHco4(WRS) and MHco10(CAVR) strains. In contrast, Hco-glc-5, Hco-avr-14, Hco-pgp-9 and Hco-dyf-7 showed no evidence of introgression in either backcross. Hco-lgc-37 and Hco-pgp-2 showed only weak evidence of introgression in the MHco3/4 backcross but not in the MHco3/10 backcross. Overall, these results suggest that microsatellite marker Hcms8a20, but not the other candidate genes tested, is linked to a major ivermectin resistance locus in the MHco4(WRS) and MHco10(CAVR) strains. This work also emphasises the need for genome-wide approaches to identify mutations responsible for the ivermectin resistance in this parasite.

show abstract

“…By identifying the alleles derived from the original resistant parent in the resulting backcrossed progeny, it was possible to generate a genetic map of resistance loci within the genome. Similar approaches have been used in mapping drug resistance associated loci in Haemonchus contortus [12, 13] and elucidating the genetic basis of drug resistance in some trematode parasite species [14]. …”

Section: Introductionmentioning

confidence: 99%

Genomic introgression mapping of field-derived multiple-anthelmintic resistance in Teladorsagia circumcincta

et al. 2017

View full text Add to dashboard Cite

Preventive chemotherapy has long been practiced against nematode parasites of livestock, leading to widespread drug resistance, and is increasingly being adopted for eradication of human parasitic nematodes even though it is similarly likely to lead to drug resistance. Given that the genetic architecture of resistance is poorly understood for any nematode, we have analyzed multidrug resistant Teladorsagia circumcincta, a major parasite of sheep, as a model for analysis of resistance selection. We introgressed a field-derived multiresistant genotype into a partially inbred susceptible genetic background (through repeated backcrossing and drug selection) and performed genome-wide scans in the backcross progeny and drug-selected F2 populations to identify the major genes responsible for the multidrug resistance. We identified variation linking candidate resistance genes to each drug class. Putative mechanisms included target site polymorphism, changes in likely regulatory regions and copy number variation in efflux transporters. This work elucidates the genetic architecture of multiple anthelmintic resistance in a parasitic nematode for the first time and establishes a framework for future studies of anthelmintic resistance in nematode parasites of humans.

show abstract

A hybridisation technique to identify anthelmintic resistance genes in Haemonchus1Note: Nucleotide sequence data reported in this paper are available in the GenBank™ database under accession numbers AF182011–AF182013.1

Cited by 35 publications

References 7 publications

Recent advances in candidate-gene and whole-genome approaches to the discovery of anthelmintic resistance markers and the description of drug/receptor interactions

Recent advances in candidate-gene and whole-genome approaches to the discovery of anthelmintic resistance markers and the description of drug/receptor interactions

Evidence from two independent backcross experiments supports genetic linkage of microsatellite Hcms8a20, but not other candidate loci, to a major ivermectin resistance locus in Haemonchus contortus

Genomic introgression mapping of field-derived multiple-anthelmintic resistance in Teladorsagia circumcincta

Contact Info

Product

Resources

About