A hybrid simulation framework for computer simulation and modelling studies of grating-based x-ray phase-contrast images

Vignero, Janne; Marshall, Nicholas; Bliznakova, Kristina; Bosmans, Hilde

doi:10.1088/1361-6560/aaceb8

Cited by 12 publications

(12 citation statements)

References 46 publications

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“…The interferometer operated at 40 kVp with a 2 μm G2 grating period and a system visibility of 22%. The radiation dose per round was 0.51 mGy [19,20]. From the acquisitions, dark field images were reconstructed through a MATLAB-algorithm.…”

Section: Dark Field Imagingmentioning

confidence: 99%

Preterm birth impairs postnatal lung development in the neonatal rabbit model

et al. 2020

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Background: Bronchopulmonary dysplasia continues to cause important respiratory morbidity throughout life, and new therapies are needed. The common denominator of all BPD cases is preterm birth, however most preclinical research in this area focusses on the effect of hyperoxia or mechanical ventilation. In this study we investigated if and how prematurity affects lung structure and function in neonatal rabbits. Methods: Pups were delivered on either day 28 or day 31. For each gestational age a group of pups was harvested immediately after birth for lung morphometry and surfactant protein B and C quantification. All other pups were hand raised and harvested on day 4 for the term pups and day 7 for the preterm pups (same corrected age) for lung morphometry, lung function testing and qPCR. A subset of pups underwent microCT and dark field imaging on day 0, 2 and 4 for terms and on day 0, 3, 5 and 7 for preterms. Results: Preterm pups assessed at birth depicted a more rudimentary lung structure (larger alveoli and thicker septations) and a lower expression of surfactant proteins in comparison to term pups. MicroCT and dark field imaging revealed delayed lung aeration in preterm pups, in comparison to term pups. Preterm birth led to smaller pups, with smaller lungs with a lower alveolar surface area on day 7/day 4. Furthermore, preterm birth affected lung function with increased tissue damping, tissue elastance and resistance and decreased dynamic compliance. Expression of vascular endothelial growth factor (VEGFA) was significantly decreased in preterm pups, however in the absence of structural vascular differences.Conclusions: Preterm birth affects lung structure and function at birth, but also has persistent effects on the developing lung. This supports the use of a preterm animal model, such as the preterm rabbit, for preclinical research on BPD. Future research that focuses on the identification of pathways that are involved in in-utero lung development and disrupted by pre-term birth, could lead to novel therapeutic strategies for BPD.

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Section: Dark Field Imagingmentioning

confidence: 99%

Preterm birth impairs postnatal lung development in the neonatal rabbit model

et al. 2020

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“…Pups were anaesthetized for the imaging with Isoflurane (2.5 MAC, 2,5%, 2l/min, ISO-VET®, EuroVet, Heusden-Zolder, Belgium) delivered via a nose cone. Dark field imaging was performed using a Talbot-Lau interferometer operating at 40 kVp with a 2 μm G2 grating period and system visibility of 22% (44). The radiation dose per round was 0.51 mGy expressed in entrance air Kerma.…”

Section: Methodsmentioning

confidence: 99%

“…The radiation dose per round was 0.51 mGy expressed in entrance air Kerma. DF and conventional attenuation images were reconstructed from the X-ray acquisitions (31,44). DF signal strength was scored in 4 lung quadrants relative to control images.…”

Section: Methodsmentioning

confidence: 99%

Intermittent CPAP limits hyperoxia-induced lung damage in a rabbit model of bronchopulmonary dysplasia

Gie

Salaets

Vignero

et al. 2020

American Journal of Physiology-Lung Cellular and Molecular Phys

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A significant proportion of preterm infants develop bronchopulmonary dysplasia (BPD) leading to poor lifelong respiratory health. Limited treatment options exist with continuous positive airway pressure (CPAP) ventilation being one of the few associated with diminished BPD. However, little is known about the effect of the distending pressure of CPAP on the developing lung exposed to hyperoxia. We aimed to identify the functional and structural effects of CPAP in a preterm hyperoxia rabbit model of BPD. Premature rabbit pups were randomized to normoxia, hyperoxia (≥95% O2), or hyperoxia plus 4 h daily CPAP [fraction of inspired oxygen (FiO2) 0.95, 5 cmH2O]. On day 7 postdelivery we performed invasive pressure-volume- and forced oscillation-based pulmonary function tests, before lung harvest for histological evaluation. Alveolar and vascular morphology, airway smooth muscle content, respiratory epithelium height, extracellular matrix components, and inflammatory cytokine expression were quantified. Hyperoxia-reared pups had restrictive lungs: alveolar walls were thickened, with the lung parenchymal tissue, collagen content, and airway smooth muscle content increased. In addition, peripheral pulmonary artery wall thickness was increased. CPAP increased alveolar recruitment and limited the structural effect of hyperoxia on the respiratory epithelium and pulmonary arteries. Additionally, CPAP improved lung function, mitigating hyperoxia-associated changes to respiratory system resistance, tissue damping, and tissue elastance. Hyperoxia disrupted functional and structural lung development. Daily intermittent CPAP limited hyperoxia-associated decreased lung function and attenuated structural changes to pulmonary arteries and respiratory epithelium while having no structural alveolar consequences. The mechanism by which CPAP has these beneficial effects needs further investigation.

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“…There is a limited number of computational studies in the field of phase-contrast grating imaging reported in the literature [19][20][21][22][23][24]. All these studies are based on the use of simple objects, such as spheres and cylinders, and aim at proof of concept and optimisation of dose and imaging parameters.…”

Section: Introductionmentioning

confidence: 99%

Computational simulations and assessment of two approaches for x-ray phase contrast imaging

Bliznakova

Dukov

2022

J. Phys.: Conf. Ser.

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X-ray phase-contrast imaging is a high-resolution imaging that permits an increase of the perceptibility of the details in three-dimensional objects, such as human tissues compared to conventional absorption imaging. There are different approaches for implementing phase-contrast imaging and their introduction into clinical practice requires advanced computational tools. A long-term goal of our research is the development of computational models of breast phase-contrast imaging. The aim of this study is to develop a software module for implementing grating-based phase-contrast imaging. For this purpose, an existing in-house software application for x-ray imaging with a function to model and simulate propagation-based phase-contrast x-ray images has been extended to include a model of grating-based imaging. To test the new functionality, four computational phantoms reflecting features, which can be screened in the real breast tissue and which differ in their complexity, were designed. Planar x-ray images in absorption, propagation-based and grating-based modes were generated and compared. Results showed improved visual appearance of the simulated objects in images obtained by simulating grating-based imaging setup. The developed subroutine is planned to be experimentally validated at synchrotron facility. The new software functionality will be exploited in studies related to new x-ray imaging techniques for breast screening and diagnosing.

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A hybrid simulation framework for computer simulation and modelling studies of grating-based x-ray phase-contrast images

Cited by 12 publications

References 46 publications

Preterm birth impairs postnatal lung development in the neonatal rabbit model

Preterm birth impairs postnatal lung development in the neonatal rabbit model

Intermittent CPAP limits hyperoxia-induced lung damage in a rabbit model of bronchopulmonary dysplasia

Computational simulations and assessment of two approaches for x-ray phase contrast imaging

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