A hybrid injectable hydrogel from hyperbranched PEG macromer as a stem cell delivery and retention platform for diabetic wound healing

Abstract

Diabetic wounds, which are a severe type of diabetes, have become one of the most serious clinical problems. There is a great promise in the delivery of adipose stem cells into wound sites using injectable hydrogels that can improve diabetic wound healing. Due to the biocompatibility of poly(ethylene glycol) diacrylate (PEGDA), we developed an in situ RAFT polymerization approach using anti-alcoholic drug-Disulfiram (DS) as a RAFT agent precursor to achieve hyperbranched PEGDA (HP-PEG). HP-PEG can form an injectable hydrogel by crosslinking with thiolated hyaluronic acid (HA-SH). ADSCs can maintain their regenerative ability and be delivered into the wound sites. Hence, diabetic wound healing process was remarkably promoted, including inhibition of inflammation, enhanced angiogenesis and re-epithelialization. Taken together, the ADSCs-seeded injectable hydrogel may be a promising candidate for diabetic wound treatment.

Physical Double‐Network Hydrogel Adhesives with Rapid Shape Adaptability, Fast Self‐Healing, Antioxidant and NIR/pH Stimulus‐Responsiveness for Multidrug‐Resistant Bacterial Infection and Removable Wound Dressing

“…Hydrogel with low swelling ratio or non‐swelling property will avoid the limitations of weak mechanical toughness caused by swelling and less medical complications, which is a desirable advantage of hydrogels being applied onto wound sites. [ 28 ] First, the morphologies of the hydrogels before and after reaching swelling equilibrium state were observed (Figure 2d). Before swelling test, hydrogel PEGSD2 showed slightly larger pore size than those of hydrogels with gelatin and GTU copolymers.…”

Macrophage-derived exosomes accelerate wound healing through their anti-inflammation effects in a diabetic rat model

“…At present, many studies have reported that the process of diabetic wound healing was affected by intense and prolonged inflammation; thus, a treatment strategy preventing inflammation shows promising potential for promoting diabetic wound healing [27,28]. A previous study demonstrates that exosomes secreted by macrophage cells influenced inflammatory pathways and contributed to the resolution of inflammation in the recipient cells [29][30][31].…”

Glycosaminoglycan and Proteoglycan Biotherapeutics in Articular Cartilage Protection and Repair Strategies: Novel Approaches to Visco‐supplementation in Orthobiologics

“…[426] Another innovative approach is the use of resident MSCs harvested from OA knee arthroscopic flushing fluids recovered from initial clinical knee examinations. This strategy yielded enhanced wound healing of diabetic wounds which have a notoriously poor repair capability similar to OA lesions in articular cartilage.…”