A human X-Y homologous region encodes “amelogenin”

Nakahori, Yutaka; Takenaka, Osamu; Nakagome, Yasuo

doi:10.1016/0888-7543(91)90251-9

Cited by 275 publications

(147 citation statements)

References 10 publications

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“…A 93-bp fragment was ampli®ed from the SRY gene, which is located solely on the Y-chromosome [19]. The ampli®cation product of the amelogenin gene is a 112-bp fragment from the Y-chromosome and a 106-bp fragment of the X-chromosome [20]. Therefore, males show two PCR products and females give a single ampli®cation product.…”

Section: Ampli®cation Of Human Dnamentioning

confidence: 99%

Molecular analysis of skeletal tuberculosis in an ancient Egyptian population

Zink

Haas

Reischl

et al. 2001

Journal of Medical Microbiology

137

104

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Section: Ampli®cation Of Human Dnamentioning

confidence: 99%

Molecular analysis of skeletal tuberculosis in an ancient Egyptian population

Zink

Haas

Reischl

et al. 2001

Journal of Medical Microbiology

137

104

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“…2B). A n amelogenin gene is located at centromere on the short arm of Y and X chromosomes (Nakahori et al, 1991). With the primer set within the amelogenin gene, a 1.…”

Section: Resultsmentioning

confidence: 99%

“…Control DNA was obtained from peripheral leukocytes of normal females and males. Oligonucleotide primers for AP-1 and AP-2, PAR-A, -B, and -C, AMXY-1F and AMXF-2R, YI-6F and Y1-4R located within the SRY (Jfiger et al, 1990b), pseudoautosomal boundary region , amelogenin gene (Nakahori et al, 1991) and DYZ1 (Nakagome et al, 1991a), were synthesized for PCR amplifications. Each nucleotide sequence of the primers is described in the Table 1.…”

Section: Patientmentioning

confidence: 99%

Sex-determining region Y (SRY) in a patient with 46,XX true hermaphroditism

et al. 1992

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SummaryUsing a polymerase chain reaction method, a search for Y-specific DNA sequences was made in samples derived from tissues of a 46,XX true hermaphrodite. We found a sequence of SRY in the ovotestis, skin and leukocytes. Other DNA sequences, which covered the pseudoautosomal boundary region, amelogenin gene and DYZ1 locus of Y-chromosome were not detected. The SRY gene detected in the patient by the polymerase chain reaction was not detected by Southern blot analysis, using the SRY fragment as a probe. These findings suggest that in the patient there is a mosaicism of cells with and without part of the Y chromosome, including the SRY sequence. As the SRY sequence was responsible for the development of the gonadal primordium to the ovotestis, SRY seems essential for gonadal differentiation in testis development.

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“…The AMELX gene, which is located on the X chromosome (Xp22.31-p22.1), makes almost all of the amelogenin. The copy of the amelogenin gene on the Y chromosome (Yp11.2), AMELY, makes very little amelogenin and is not required for enamel formation [7].…”

Section: Introductionmentioning

confidence: 99%

Dimorphic Goat Amelogenin Alleles on Sex Chromosomes for Gender Determination of Preimplantation Embryos

Chen¹,

Tsai²,

JanChi³

et al. 2013

IJBBB

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 Abstract-Amelogenin (AMEL) is a conserved gene located on the sex chromosomes of mammals. It is involved in the formation of enamel, which is the hard, white material that forms the protective outer layer of each tooth. In this study, we first cloned the coding sequences of the goat AMELX and AMELY transcripts from female and male lamb enamel tissues during tooth development. All 207 amino acids of the putative AMEL proteins from the cDNA coding regions of both sex chromosomes were the same, but the AMEL sequences of the 3'-untranslated regions (UTR) were different. The results showed that the nucleotide sequences of intron 5 of the goat AMELX and AMELY genes contained multiple deletions/insertions and had only 48.5% identity. Based on the dimorphic AMEL intron sequences, a set of sex-specific primers was successfully applied to goat gender determination. A high sensitivity for sex determination was reached with a minimal amount of template, such as a trace amount of genomic DNA from a single blastomere isolated from embryos at the blastula stage among different strains of goats.

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A human X-Y homologous region encodes “amelogenin”

Cited by 275 publications

References 10 publications

Molecular analysis of skeletal tuberculosis in an ancient Egyptian population

Molecular analysis of skeletal tuberculosis in an ancient Egyptian population

Sex-determining region Y (SRY) in a patient with 46,XX true hermaphroditism

Dimorphic Goat Amelogenin Alleles on Sex Chromosomes for Gender Determination of Preimplantation Embryos

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