1983
DOI: 10.1093/nar/11.9.2551
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A human tRNAGlugene of high transcriptional activity

Abstract: A mixture of low molecular weight RNAs, in which only tRNAs were radiolabelled, was used as a hybridisation probe to select for tRNA-like sequences within a bank of human genomic DNA in lambda Charon 4A. A restriction enzyme digest of one of the selected lambda Charon 4A recombinants contained two fragments (2.4 Kb & 1.8 Kb) which hybridised tRNA and which, when subcloned into pAT153, were transcribed in Xenopus oocyte nuclei. Analysis of the subcloned 2.4 Kb fragment, which was of remarkably high transcriptio… Show more

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Cited by 35 publications
(15 citation statements)
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“…There is a mismatch present within the anticodon stem which is abnormal but not impossible. A similar mismatch has been reported for human tRNAG'" [45] and such a mismatch is also present in the tRNApdA gene from Dictyostelium (Dingermann and Sogin, unpublished). Furthermore the D-stem has only two base pairs while three or four are normal and within its long extra arm no significant base pairing occurs.…”
Section: Discussionsupporting
confidence: 78%
See 1 more Smart Citation
“…There is a mismatch present within the anticodon stem which is abnormal but not impossible. A similar mismatch has been reported for human tRNAG'" [45] and such a mismatch is also present in the tRNApdA gene from Dictyostelium (Dingermann and Sogin, unpublished). Furthermore the D-stem has only two base pairs while three or four are normal and within its long extra arm no significant base pairing occurs.…”
Section: Discussionsupporting
confidence: 78%
“…Therefore the unusual arrangement of the tRNAval gene does not lead to very efficient transcription in the Xenopus germinal vesicle extract. Goddart et al reported recently a transcriptionally very active human tRNAG'" gene, which was also associated in its 5' flank with a tRNA-like structure [45]. This tRNA-like structure, however, did not overlap with the tRNAG'" gene nor was it cotranscribed with this gene.…”
Section: Discussionmentioning
confidence: 99%
“…pRH5.7 contains a human Alu gene (57). pGlu6 contains a human tRNA Glu gene (11). pTI contains the TATA box and initiator element of the adenovirus major late promoter (33).…”
Section: Methodsmentioning
confidence: 99%
“…pGlu6 contains a HindIII fragment including a human tRNA Glu gene inserted into pAT153 (28). pHu5S3.1 contains a 638-base pair BamHI-SacI fragment from human genomic DNA including a 5 S rRNA gene, inserted into pBluescript SKϩ from Stratagene (29).…”
Section: Plasmids-mentioning
confidence: 99%