A human sperm-zona pellucida binding test using oocytes that failed to fertilize in vitro

Liu, De Yi; Lopata, Alexander; Johnston, W. I. H.; Baker, H. W. G.

doi:10.1016/s0015-0282(16)60316-3

Cited by 146 publications

(54 citation statements)

References 20 publications

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“…S p e r m b i n d i n g t o h o m o l o g o u s Z P i s a prerequisite for fertilization, and tests that evaluate this function have been described for several species (the cat [14][15][16], the dog [17], the pig [18], and the human [19]). Spermatozoa attachment to the ZP, a cellular glycoprotein coat surrounding oocytes, is a crucial step in mammalian fertilization.…”

Section: Discussionmentioning

confidence: 99%

Sperm Motility, Plasma Membrane Integrity, and Binding Capacity to Homologous Zona Pellucida of Cryopreserved Epididymal Spermatozoa in the Domestic Cat

Kashiwazaki

Yamaguchi

Uesugi³

et al. 2005

Journal of Reproduction and Development

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Abstract. We examined motility, plasma membrane integrity, and binding capacity to homologous zona pellucidae (ZP) of frozen/thawed epididymal cat sperm as a model species for endangered felines. Epididymal spermatozoa from 20 domestic cats were frozen with freezing egg-yolk extender containing 3.0% glycerol in 0.25-ml straws. Post-thaw motility and plasma membrane integrity of the frozen/thawed spermatozoa were 31.8 ± 2.4% and 32.2 ± 4.2%, respectively. The frozen/thawed spermatozoa were co-cultured with frozen/thawed immature homologous oocytes with intact ZP for 3 h to examine their ability to bind to the ZP. Sixteen of the 20 frozen/thawed sperm samples demonstrated the ability to bind to ZP. These results indicated that the freezing system for epididymal sperm used in the present study gives appropriate information for banking the genetic resources of wild felid species. Key words: Cat, Epididymal sperm, Freezing, Zona pellucida binding (J. Reprod. Dev. 51: [735][736][737][738][739] 2005) xcluding the domestic cat, all of the felidae f a m i l y a r e c o n s i d e r e d t h r e a t e n e d o r endangered, including Iriomote wild cats (Felis iriomotensis) and Tsushima leopard cats (Felis bengalensis euptilura), which are both mammals native to Japan [1]. Effective conservation and maintenance of an adequate diversity of animals can be achieved by preserving gametes and/or embryos as genetic resources in order to prevent the extinction of species [2]. Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males that die unexpectedly, thus preserving gametes that could be used to maintain genetic variety within depleted

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Section: Discussionmentioning

confidence: 99%

Sperm Motility, Plasma Membrane Integrity, and Binding Capacity to Homologous Zona Pellucida of Cryopreserved Epididymal Spermatozoa in the Domestic Cat

Kashiwazaki

Yamaguchi

Uesugi³

et al. 2005

Journal of Reproduction and Development

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“…In these studies, ZBTs have been performed either with fresh immature or mature oocytes or with aged unfertilized IVF oocytes, which were pooled until use in a concentrated salt solution or cryopreserved. In these cases, immaturity, pooling, storage, aging, or previous exposure of the zonae to sperm seemed not to affect the results of the ZBTs adversely (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

“…The human sperm-zona pellucida binding test (ZBT) has been developed using a hemizona (1=3) as well as an intact zona (4)(5)(6) model. This test has been used either to investigate the molecular mechanisms of human gamete recognition (7)(8)(9)(10)(11)(12)(13)(14) or as a clinical assay to determine the potential use of a patient sperm in in vitro fertilization (IVF) attempts (15)(16)(17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Aging, hyaluronidase removal of the cumulus, and microinjection do not affect the sperm binding potential of human oocytes

Barros

Sousa

Silva

et al. 1997

J Assist Reprod Genet

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“…Some of these functions are achievement of the capacitation and the getting ready for the beginning of the acrosome reaction. [13,14] HZA [15] and a competitive intact zona sperm binding assay [16] are the two most commonly used sperm-ZP binding tests ( Figure 3). Although the methodologies are different, they evaluate the tight binding of sperm to ZP as primary outcome.…”

Section: Sperm-zona Pellucida Binding Testsmentioning

confidence: 99%

Sperm function tests in clinical practice

Kızılay¹,

Altay²

2017

Turkish Journal of Urology

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Conventional semen analysis solely is not completely adequate to predict pregnancy outcomes. Therefore, advanced sperm function tests have been developed and introduced to clinical practice. These tests use different methods and techniques to evaluate different stages of fertilization steps. In this review, we reported some commonly used sperm function tests: sperm penetration assay, sperm-zona pellucida binding test (hemizona assay), acrosomal reaction test, hyaluronan binding test, hypo-osmotic swelling test, magneticactivated cell sorting and zeta sperm selection. We discussed the literature concerning these tests, the utilization techniques and also purpose and mechanism of each test. We emphasized the importance of sperm function tests in predicting in vitro fertilization and pregnancy outcomes and in the management of infertile couples and also the limitations of these tests. Along with improvements in molecular biology techniques, we believe that more applicative and beneficial tests will be developed in the near future.

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A human sperm-zona pellucida binding test using oocytes that failed to fertilize in vitro

Cited by 146 publications

References 20 publications

Sperm Motility, Plasma Membrane Integrity, and Binding Capacity to Homologous Zona Pellucida of Cryopreserved Epididymal Spermatozoa in the Domestic Cat

Sperm Motility, Plasma Membrane Integrity, and Binding Capacity to Homologous Zona Pellucida of Cryopreserved Epididymal Spermatozoa in the Domestic Cat

Aging, hyaluronidase removal of the cumulus, and microinjection do not affect the sperm binding potential of human oocytes

Sperm function tests in clinical practice

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