A Human Lung-Associated Streptomyces sp. TR1341 Produces Various Secondary Metabolites Responsible for Virulence, Cytotoxicity and Modulation of Immune Response

Abstract

AH made the genetic manipulations with the streptomycete strain, performed fermentations and cocultures with human cells, prepared the metabolic extracts, and performed cytotoxicity and immunomodulatory effects assays of the extracts. EC performed the phylogenetic analyses, analyzed the genomic data and made the hemolysis and growth-inhibitory activities assays in vivo. AC supervised the phylogenetic analyses and analyzed the data, performed the genome sequencing, and participated in the manuscript preparation. HL, PP, and JH assayed the human cells-targeted activities. HL designed and performed the in vivo cocultures and evaluated their effects. PP, JH, and AH designed and performed the experiments to assess immunomodulatory features of metabolic extracts and their cytotoxicity. JH and HL evaluated the human cells-related experiments data and participated in the manuscript preparation. MČ performed and evaluated the LC-MS experiments. VK made the strain taxonomic and morphologic characterization. JB was involved in the growth-inhibitory activities in vivo and in evaluation of the strain interactions with

A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell wall acetylation

“…As a class of polyketide synthase products, aryl polyenes are lipids with an aryl head group conjugated to a polyene tail (Lin et al, 2015) and are widely distributed in soil and host-associated bacteria (Cimermancic et al, 2014; Youngblut et al, 2020). Although, many polyene compounds isolated from terrestrial and marine microbes possess antimicrobial affects in vitro (Herbrík et al, 2020; Lee et al, 2020; Li et al, 2021; Zhao et al, 2021), virtually nothing is known about their ecological role in microbe-host and microbe-microbe interactions in the mammalian gastrointestinal tract (Aleti et al, 2019). While metagenome studies are critically important to identify novel polyene-like BGCs within the microbiome (Hiergeist et al, 2015; Medema et al, 2011), the assessment of model organisms and their isogenic mutants in the appropriate ecological context is critical to advance our knowledge on the biological function of these BGC-derived compounds.…”