A human endometrial explant system: Validation and potential applications

Dudley, Donald J.; Hatasaka, Harry H.; Branch, D. Ware; Hammond, Elizabeth; Mitchell, Murray D.

doi:10.1016/0002-9378(92)91774-5

Cited by 21 publications

(17 citation statements)

References 16 publications

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“…The endometrial explant model was used to identify mifepristone-regulated immunomodulation of receptive endometrium. Previous studies showed that tissue viability and responsiveness was maintained in this endometrial explants system for 24 hours or more and explants responded to steroids in a manner similar to that of endometrium in vivo (13,14). The concentrations of E 2 and P in these endometrial explants closely resembled plasma concentrations reported during the midsecretory phase of the endometrium (15).…”

Section: Explant Culturesupporting

confidence: 62%

Low-dose mifepristone increases uterine natural killer cell cytotoxicity and perforin expression during the receptive phase

Zhou¹,

Chen²,

Zhuang³

et al. 2011

Fertility and Sterility

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Section: Explant Culturesupporting

confidence: 62%

Low-dose mifepristone increases uterine natural killer cell cytotoxicity and perforin expression during the receptive phase

Zhou¹,

Chen²,

Zhuang³

et al. 2011

Fertility and Sterility

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“…Our observations concerning the morphology of epithelial cells correspond to the published validation study [1] where the viability of these cells over 4 days was documented. Later in the course, apoptotic features, like the condensation of nuclear chromatin and autophagy, are seen.…”

Section: Discussionsupporting

confidence: 87%

“…In conclusion, we have observed in this preliminary study that, in contrast to the validation study of Dudley [1], the endometrial explant system is limited by an early decay of stromal cells in the absence of added progesterone. Products of stromal cells, like prolactin and IGF-bp1, are detected in the supernatant of endometrial explant cultures, which could be due at least in part to early cell decay and the release of (Fig.…”

Section: Discussioncontrasting

confidence: 52%

“…These systems maintain their three-dimensional structure and are competent for the de novo synthesis of endometrial proteins. The morphological validation for epithelial cells in explants cultured (for more than 4 days) has been provided by Dudley et al [1], but no such validation has been given on stromal cells neither for morphological criteria nor for the specific production of biochemical markers.…”

Section: Introductionmentioning

confidence: 99%

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Morphology of human endometrial explants and secretion of stromal marker proteins in short- and long-term cultures

et al. 2009

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Human endometrial tissue is frequently biopsied under surgical and laparoscopic procedures for the investigation of infertility, abdominal, or menstrual pain. These symptoms often but not always are the consequence of endometriosis, which is characterised by the growth of endometrial tissue outside the uterine cavity and affecting 8-10% of women during the fertile age. First-line treatment is often by surgery. Biopsied endometrial tissue is not only used for immunohistochemical examination but has also been cultured in vitro. Explant culture systems maintain the three-dimensional structure of the tissue, but so far no morphological validation studies are available for the stromal cells which are responsible for the production of hormones and inflammatory cytokines in the endometrium. We have documented, by transmission electron microscopy, the morphological alterations of stromal cells in short-(12 h) and long-term (7 days) cultures of endometrial explants biopsied in the postovulatory phase. The production of prolactin, a stromal cell marker, was determined. We found that the morphological integrity of these cells was starting to be disrupted from as early as 12 h in culture. Some stromal cells, however, developed into predecidual cells. After 96 h, a large fraction of the cell population was necrotic, and after 7 days, the cytoplasm had disappeared. In presence of progesterone, the decay of stromal cell integrity was slowed down. The release of prolactin and IGF-binding protein-1 during culture followed the morphological pattern. We conclude that the explant culture model is viable for not more than 48 h in vitro for stromal cells, but that this interval can be prolonged by the addition of progesterone which initiates decidualisation.

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“…Several approaches are feasible. Endometrial cells or tissue fragments can be cultured on feeder layers of several cell types, or on isolated ECM components [15][16][17]. Many established cell lines or primary cell cultures have been grown on coatings of ECM components such as collagen I, laminin, fibronectin [18].…”

Section: Introductionmentioning

confidence: 99%

Amniotic Membrane as an in vitro Model for Endometrium- Extracellular Matrix Interactions

Linden

Goeij

Dunselman

et al. 1998

Gynecol Obstet Invest

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The aim of the study was to develop an in vitro model to study the interaction between endometrial cells and extracellular matrix (ECM) and to evaluate the expression of cell adhesion molecules in endometrial cells and tissue fragments under in vitro conditions. Endometrial biopsies were collected from 32 patients. Samples were either digested using collagenase type I, or dissected mechanically. Adhesion of isolated cells and tissue fragments to stripped amniotic membranes and to coverslips coated with ECM components was studied. The expression of β1 integrins and cadherins was assessed using immunohistochemistry. Collagenase digestion of endometrial biopsies yielded viable single cells. These cells did not adhere to either side of stripped amniotic membranes, and did not show expression of the cell adhesion molecules. In contrast, mechanically fragmented endometrium samples adhered to both sides of stripped amniotic membranes and showed immunohistochemical expression of E- and P-cadherin and integrin subunits α2, α3, α4, α5 and α6. Amniotic membranes, after stripping of epithelial lining, are suitable to study interactions between endometrial tissue and ECM in functional and structural studies. Endometrial cells after collagenase type I digestion do not adhere to stripped amniotic membrane and have lost expression of β1 integrins and E- and P-cadherin.

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A human endometrial explant system: Validation and potential applications

Cited by 21 publications

References 16 publications

Low-dose mifepristone increases uterine natural killer cell cytotoxicity and perforin expression during the receptive phase

Low-dose mifepristone increases uterine natural killer cell cytotoxicity and perforin expression during the receptive phase

Morphology of human endometrial explants and secretion of stromal marker proteins in short- and long-term cultures

Amniotic Membrane as an in vitro Model for Endometrium- Extracellular Matrix Interactions

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