A human colon tumour antigen associated with β2-Microglobulin and isolated from solid tumour, serum and urine, is unrelated to carcinoembryonic antigen

Thomson, David M.; Tataryn, Donna N.; Weatherhead, Josie C.; Friedlander, P. H.; Rauch, Joyce; Schwartz, Rosemarie; Gold, Phil; Shuster, Joseph

doi:10.1016/0014-2964(80)90234-0

Cited by 8 publications

(2 citation statements)

References 27 publications

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“…Grosser and Thomson [7] and Thomson et al [37,38] have reported similar findings. Preincubation of PBL from patients with limited breast cancer, colon cancer, or melanoma with sera from patients with advanced disease significantly decreased the basal glass adherence of treated PBL in an immunologically specific manner.…”

Section: Discussionsupporting

confidence: 60%

Regulation of leukocyte glass adherence and tube leukocyte adherence inhibition (LAI) reactivity by serum factors in dogs with progressing or spontaneously regressing canine transmissible venereal sarcoma (CTVS)

Harding

Yang

1985

Cancer Immunol Immunother

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We examined the regulation of leukocyte glass adherence and tube leukocyte adherence inhibition (LAI) reactivity by serum factors in dogs with regressing or progressing canine transmissible venereal sarcomas (CTVS). Both regressor and progressor peripheral blood leukocytes (PBL), draining and non-draining lymph node cells (LNC), and splenic leukocytes were significantly responsive to CTVS antigen extract in tube LAI. In contrast, a significant decrease in basal glass adherence of progressor PBL, draining and non-draining LNC, and splenic leukocytes was observed. Normal glass adherence was restored to progressor leukocytes by extensive washing with warm serum-free media, while significant tube LAI responsiveness to CTVS antigen extract was maintained. Preincubation of regressor PBL and LNC with progressor sera in two-stage tube LAI decreased the basal glass adherence of treated leukocytes. This effect of progressor sera was heat labile, a characteristic of CTVS antigen. Collectively, these findings suggest that progressor leukocytes and progressor sera treated regressor leukocytes were activated by interaction with serum CTVS antigen and thus behaved in tube LAI as stimulated cells, even in the absence of CTVS antigen. Regressor but not progressor sera were shown to contain anti-CTVS IgG with specific arming activity for normal dog PBL, but not LNC in two-stage tube LAI. The nonadherent response of peripheral blood neutrophils in two-stage tube LAI was proportional to the concentration of arming IgG, whereas no change was observed in glass adherence of PBL. The results of this study define the role of progressor and regressor serum factors in the mechanism of tube LAI and demonstrate a relationship between leukocyte glass adherence and the clinical course of CTVS. These findings show that tube LAI is a simple and reproducible measure of active factors in the immune response to a tumor.

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Section: Discussionsupporting

confidence: 60%

Regulation of leukocyte glass adherence and tube leukocyte adherence inhibition (LAI) reactivity by serum factors in dogs with progressing or spontaneously regressing canine transmissible venereal sarcoma (CTVS)

Harding

Yang

1985

Cancer Immunol Immunother

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“…In addition, the organ-type specific neoantigens (TSA) of human cancer are linked to Pz microglobulin.22~23~25 The colon cancer TSA that is linked to Pzm and recognized by the LAI-response is a molecule that is distinct from CEA. 27 Colonic adenoma and its variants have been shown to be significantly associated with carcinoma.2 Furthermore, most if not all colonic carcinoma begins within a nexus of adenoma.2 The likelihood of finding carcinoma increases with the size of the adenoma; thus, larger adenomas may be considered a statistically significant precancerous tissue for large bowe! carcinoma.2 In the present study, 3 of 16 (19%) patients with adenomas showed LA1 reactivity on two separate tests before surgery that disappeared when the adenoma was removed.…”

Section: Cancer March 1979mentioning

confidence: 99%

Tube leukocyte adherence inhibition (LAI) assay in gastrointestinal (GIT) cancer

Tataryn

MacFarlane

Murray

et al. 1979

Cancer

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Tumor-specific immunity to carcinoma of the colon, pancreas and stomach was assayed by tube LAI. Cancers of the colon, pancreas and stomach, were shown to possess organ-type specific neoantigens. In 115 patients with colon cancer, loo%, 75%, 61% with Dukes' A, B and C cancer were LA1 positive, respectively. Even a microfocus of in situ cancer in a colon adenoma was sufficient to stimulate measurable tumor-specific immunity in the host. In Dukes' D cancer, 25% of patients with widespread metastasis were positive, whereas 100% with solitary lesions were positive. Reactive leukocytes from patients with colon cancer did not react to extracts of normal bowel mucosa or villous adenoma from LAI-negative patients. Leukocytes from 19% (3 of 16) of patients with colon adenomas reacted to the extract of colon cancer but not normal colon mucosa. Moreover, the LAI-positive response of the patients with colon adenomas or colon cancer is directed to a colon cancer TSA which is linked to µglobulin. These studies suggest that some colon adenomas express TSA before morphological evidence of cancer. It is not known if the acquisition of a cell surface TSA is an irreversible step toward unrestrained growth and metastasis. In pancreatic cancer, 100% of patients with cancers less than 5 cm and without metastasis were LA1 positive, whereas 29% were positive when the cancer was >5 cm or had metastasized. In Patients with stomach cancer, 100% with Stage I1 and 46% with Stage I11 and IV cancer were LAI-positive. Leukocytes from patients with other GIT cancers and from patients with inflammatory bowel disease or pancreatitis did not react with extracts of colon, stomach or pancreatic cancer. Leukocytes, from patients with metastatic cancer, usually did not react in the tube LA1 assay because their surfaces were coated in vivo with TSA. LA1 reactivity was present when CEA was not detectable and when CEA levels were elevated LA1 activity was often absent. The present study suggests that the automated tube LA1 shows sufficient promise to warrant studies to determine its efficacy for the diagnosis of GIT cancers.Cancer 43:898-912, 1979.

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Gastrointestinal Cancer Markers

Sjögren

Wahrén

1982

Human Cancer Markers

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A human colon tumour antigen associated with β2-Microglobulin and isolated from solid tumour, serum and urine, is unrelated to carcinoembryonic antigen

Cited by 8 publications

References 27 publications

Regulation of leukocyte glass adherence and tube leukocyte adherence inhibition (LAI) reactivity by serum factors in dogs with progressing or spontaneously regressing canine transmissible venereal sarcoma (CTVS)

Regulation of leukocyte glass adherence and tube leukocyte adherence inhibition (LAI) reactivity by serum factors in dogs with progressing or spontaneously regressing canine transmissible venereal sarcoma (CTVS)

Tube leukocyte adherence inhibition (LAI) assay in gastrointestinal (GIT) cancer

Gastrointestinal Cancer Markers

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