A human capsaicin model to quantitatively assess salivary CGRP secretion

Oosterhout, Willebrordus P J van; Schoonman, GG; Garrelds, Ingrid M.; Danser, A.H. Jan; Chan, Kayi Y.; Terwindt, GM; Ferrari, Michel D.; MaassenVanDenBrink, Antoinette

doi:10.1177/0333102414553824

Cited by 13 publications

(6 citation statements)

References 38 publications

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“…Demirbilek et al (2004), for example, observed that subcutaneously administered capsaicin increased plasma CGRP concentration in rats. Oral administration of a red chili homogenate in human subjects also increased CGRP concentration in a dose-dependent manner (van Oosterhout et al, 2015). In the current experiment, RPC may have stimulated CGRP production and decreased insulin secretion.…”

Section: Discussionsupporting

confidence: 49%

Effects of rumen-protected Capsicum oleoresin on productivity and responses to a glucose tolerance test in lactating dairy cows

Harper

Giallongo

et al. 2017

Journal of Dairy Science

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The objective of this experiment was to investigate the effects of rumen-protected Capsicum oleoresin (RPC) supplementation on feed intake, milk yield and composition, nutrient utilization, fecal microbial ecology, and responses to a glucose tolerance test in lactating dairy cows. Nine multiparous Holstein cows were used in a replicated 3 × 3 Latin square design balanced for residual effects with three 28-d periods. Each period consisted of 14 d for adaptation and 14 d for data collection and sampling. Treatments were 0 (control), 100, and 200 mg of RPC/cow per day. They were mixed with a small portion of the total mixed ration and top-dressed. Glucose tolerance test was conducted once during each experimental period by intravenous administration of glucose at a rate of 0.3 g/kg of body weight. Dry matter intake was not affected by RPC. Milk yield tended to increase for RPC treatments compared to the control. Feed efficiency was linearly increased by RPC supplementation. Concentrations of fat, true protein, and lactose in milk were not affected by RPC. Apparent total-tract digestibility of dry matter, organic matter, and crude protein was linearly increased, and fecal nitrogen excretion was linearly decreased by RPC supplementation. Rumen-protected Capsicum oleoresin did not affect the composition of fecal bacteria. Glucose concentration in serum was not affected by RPC supplementation post glucose challenge. However, compared to the control, RPC decreased serum insulin concentration at 5, 10, and 40 min post glucose challenge. The area under the insulin concentration curve was also decreased 25% by RPC. Concentration of nonesterified fatty acids and β-hydroxybutyrate in serum were not affected by RPC following glucose administration. In this study, RPC tended to increase milk production and increased feed efficiency in dairy cows. In addition, RPC decreased serum insulin concentration during the glucose tolerance test, but glucose concentration was not affected by treatment.

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Section: Discussionsupporting

confidence: 49%

Effects of rumen-protected Capsicum oleoresin on productivity and responses to a glucose tolerance test in lactating dairy cows

Harper

Giallongo

et al. 2017

Journal of Dairy Science

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“…The inhibitory effect of capsaicin on insulin may be exhibited via CGRP, which is known to decrease insulin secretion by the pancreas (Pettersson and Ahrén, 1990;Tanaka et al, 2013). Capsaicin treatments reportedly increased plasma CGRP concentration in rats and human subjects (Demirbilek et al, 2004;van Oosterhout et al, 2015). Hashemzadeh-Cigari et al (2015) reported increased insulin sensitivity in dairy cows fed a mixture of PN including rosemary, cinnamon bark, turmeric, and clove bud.…”

Section: Hormone Regulationmentioning

confidence: 99%

Host-mediated effects of phytonutrients in ruminants: A review

Wall²,

Bravo³

et al. 2017

Journal of Dairy Science

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Plants produce an extensive array of organic compounds derived from secondary metabolism that may be useful in animal nutrition because of their chemical makeup. These plant-derived bioactive compounds, also referred to as phytonutrients (PN) or phytobiotics, have been shown to express antimicrobial activities against a wide range of bacteria, yeast, and fungi and have been investigated as rumen modifiers in ruminant nutrition. Studies have reported that PN may inhibit deamination of AA and methanogenesis in the rumen and shift fermentation toward propionate and butyrate. Most of the experiments, however, have been conducted in vitro, and responses have been highly variable and inconsistent in animal experiments. In addition, some studies have reported that PN had positive effects on productivity, although rumen fermentation was not affected. Other than antimicrobial effects in the gut, PN are known to bind specific receptors expressed in neurons, intestines, and other cells and exhibit related physiological effects in nonruminants. The receptor-mediated effects include immune responses, oxidative stress, and insulin secretion and activity. Some PN, due to their phenolic nature, are likely less susceptible to microbial degradation in the rumen and may exhibit activities postruminally, similar to their mode of action in nonruminant species. This opens a new area of research in ruminants, including effects of PN on the animal's immune system, postruminal nutrient use, and animal physiology. Although limited, studies with ruminants provide first evidence of PN's regulatory effects on the host responses. For example, PN were reported to regulate immune cells related to adaptive and innate immunity in challenged or nonchallenged dairy cows. Supplementation of PN reduced oxidative stress by decreasing lipid peroxidation and increasing endogenous antioxidants in ruminants. Additionally, insulin secretion and sensitivity were reportedly regulated by PN in dairy cows. The regulatory effects of PN on immunity may be beneficial for immune suppression and inflammation in dairy cows. In addition, PN could positively affect energy partitioning for milk production through their effects on insulin secretion and sensitivity. Further research is needed to elucidate the effect and mode of action of PN on immune function and animal energetics.

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“…It is worth mentioning that these two groups with different capsaicin dosages showed some differences in the CGRP expression levels at 12 h. The mechanism behind this effect might be the dose-dependent release of CGRP induced by excitation of TRPV1 24 . This could also be supported by the observation that application of capsaicin caused a dose-dependent release of salivary CGRP, which might reflect the activated state of trigeminal system 27 . Moreover, in our study, we found that following experimental tooth movement, CGRP expression levels both in TG and Vc at 1 d and 3 d were significantly lower than that in the saline+force group.…”

Section: Discussionmentioning

confidence: 82%

The effect of capsaicin on expression patterns of CGRP in trigeminal ganglion and trigeminal nucleus caudalis following experimental tooth movement in rats

Zhou

Long

et al. 2016

J. Appl. Oral Sci.

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Objectives The aim of this study was to explore the effect of capsaicin on expression patterns of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) and trigeminal subnucleus caudalis (Vc) following experimental tooth movement.Material and Methods Male Sprague-Dawley rats were used in this study and divided into small-dose capsaicin+force group, large-dose capsaicin+force group, saline+force group, and no force group. Closed coil springs were used to mimic orthodontic forces in all groups except for the no force group, in which springs were inactivated. Capsaicin and saline were injected into periodontal tissues. Rats were euthanized at 0 h, 12 h, 1 d, 3 d, 5 d, and 7 d following experimental tooth movement. Then, TG and Vc were obtained for immunohistochemical staining and western blotting against CGRP.Results Immunohistochemical results indicated that CGRP positive neurons were located in the TG, and CGRP immunoreactive fibers were distributed in the Vc. Immunohistochemical semiquantitative analysis and western blotting analysis demonstrated that CGRP expression levels both in TG and Vc were elevated at 12 h, 1 d, 3 d, 5 d, and 7 d in the saline + force group. However, both small-dose and large-dose capsaicin could decrease CGRP expression in TG and Vc at 1 d and 3 d following experimental tooth movement, as compared with the saline + force group.Conclusions These results suggest that capsaicin could regulate CGRP expression in TG and Vc following experimental tooth movement in rats.

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A human capsaicin model to quantitatively assess salivary CGRP secretion

Abstract: Oral application of red chili homogenate is well tolerated and causes a dose-dependent CGRP release in saliva, without day-to-day effects in this response. This model could be used to noninvasively study the activation state of the trigeminal nerve innervating salivary glands.

Cited by 13 publications

References 38 publications

Effects of rumen-protected Capsicum oleoresin on productivity and responses to a glucose tolerance test in lactating dairy cows

Effects of rumen-protected Capsicum oleoresin on productivity and responses to a glucose tolerance test in lactating dairy cows

Host-mediated effects of phytonutrients in ruminants: A review

The effect of capsaicin on expression patterns of CGRP in trigeminal ganglion and trigeminal nucleus caudalis following experimental tooth movement in rats

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