A human B‐lineage dendritic cell line, HBM‐Noda and its potential role in human T‐cell leukemia/lymphoma virus type I infection

Nagasaki, Makoto; Morikawa, Shigeru; Torii, Ikuko; Zhang, Jie; Morikawa, Keiko

doi:10.1046/j.1440-1827.2000.01040.x

Cited by 6 publications

(24 citation statements)

References 41 publications

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“…49,50 The kidneyderived antigens and subtypes of lymphocytes appearing in relation to OX6-positive cells remain to be investigated. In addition, a subset of B cells has been reported to give rise to dendritic morphology and functions, 4,24 although dendritic cells are generally considered to develop from bone marrow myeloid lineage precursors. 31,37 Further study of the derivation of OX6-positive cells, in particular the association between OX6-positive interstitial cells and B cells, would be interesting.…”

Section: Discussionmentioning

confidence: 99%

Participation of Different Macrophage Populations and Myofibroblastic Cells in Chronically Developed Renal Interstitial Fibrosis after Cisplatin-induced Renal Injury in Rats

et al. 2002

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Abstract. To shed some light on the mechanisms behind renal fibrogenesis, the present study immunohistochemically investigated the participation of different macrophage populations and myofibroblastic cells in rat renal interstitial fibrosis developed chronically after repeated injection of cisplatin (2 mg/kg body weight, once weekly for 7 weeks). During the 19-week recovery period after the final injection, fibrotic lesions progressively developed in the corticomedullary junction, with the greatest level at post-final injection (FPI) week 5, and then the lesions were gradually repaired by PFI week 19, indicative of a healing process. In conformity with the development of fibrotic lesions, the number of myofibroblastic cells reacting with an anti-␣-smooth muscle actin antibody was increased, with a peak at PFI week 3, and collagens (types I, III, and IV), fibronection, and laminin were excessively accumulated in these areas. Interstitial cells forming the fibrotic lesions showed mitotic activity at the early stages, whereas they disappeared by apoptosis in the healing process. A large number of cells reacting with an antibody of ED1 (for exudate macrophages), ED2 (for resident macrophages), or OX6 (for major histocompatibility complex class II-presenting macrophages and interstitial dendritic cells) had already appeared at PF1 week 1, and then their numbers increased, with a peak at PFI weeks 7, 3, and 9 in ED1-, ED2-, and OX6-positive cells, respectively. Thereafter, the number of ED1-and ED2-positive cells decreased, whereas the number of OX6-positive cells persisted at a high level until PFI week 19. In the healing process, clusters of lymphocytes were present, the development of which might have been related to OX6-positive cells. The present study demonstrated that chronically developing rat renal interstitial fibrosis might be produced by the complicated mechanisms evoked by interactions between different macrophage populations and myofibroblastic cells, because macrophages show heterogeneous functions depending on microenvironmental factors.

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Section: Discussionmentioning

confidence: 99%

Participation of Different Macrophage Populations and Myofibroblastic Cells in Chronically Developed Renal Interstitial Fibrosis after Cisplatin-induced Renal Injury in Rats

et al. 2002

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“…HBM‐Noda (Noda) was established from bone marrow‐aspiration fluid of a 68‐year‐old myelodysplastic syndrome patient in 1990 by Morikawa et al 17 . Ruffled projections and invaginated nucleus were observed in Noda by the phase contrast microscopy as well as by light microscopy.…”

Section: Methodsmentioning

confidence: 99%

“…Noda had locomotive and adhesive capacities in the Petri dish. Noda showed a strong capacity to stimulate E rosette‐purified allogenic T cells and the capacity was more effective than that of peripheral blood non‐T cells or malignant B lymphoma cell lines 17 . HPL‐Hod‐1 (Hod‐1) was established from pleural effusion of a 57‐year‐old Hodgkin's disease patient in 1972 by Morikawa et al 18 .…”

Section: Methodsmentioning

confidence: 99%

“…demonstrated the existence in normal human adult and fetal bone marrow, of a novel CD34 + progenitor cells which have the ability to produce B cells, T cells, NK and DC. Previously, we also have established a unique cell line, HBM‐Noda (Noda), 17 which was derived from bone marrow cells of a myelodysplastic syndrome patient. The most characteristic feature of Noda is that it has some B‐cell specific properties (immunoglobulin G (IgG) production, immunoglobulin gene rearrangement, some B‐cell surface markers) as well as DC specific properties (dendritic morphology, alloantigen stimulation, DC surface markers).…”

Section: Introductionmentioning

confidence: 99%

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Differential endocytotic characteristics of a novel human B/DC cell line HBM‐Noda: effective macropinocytic and phagocytic function rather than scavenging function

Torii¹,

Morikawa²,

Nagasaki³

et al. 2001

Immunology

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SUMMARYIn order to characterize a novel human B cell-lineage dendritic cell line (B/DC line) as an antigenpresenting cell (APC), we compared three types of endocytosis (micropinocytosis via a clathrincoated pit, macropinocytosis via membrane ruf¯ing, and phagocytosis) among myeloid-related, macrophage (Mw) cell lines and a B/DC line. In the present examination, we used a unique human dendritic cell (DC) line, HBM-Noda (Noda). Flow cytometric and immunocytochemical analyses revealed that Noda not only expresses some DC markers, but also it expresses some B-cell associated markers. Noda shows strong capacities to stimulate allogenic T cells, to produce immunoglobulin G (IgG), and to perform immunoglobulin gene rearrangment. These data strongly suggest that Noda is a B-cell lineage DC line. The endocytic differences among these cell lines were as follows. (1) The level of micropinocytosis of Noda was signi®cantly less than that of conventional human Mw cell lines, and the formation of a clathrin-coated pit was not observed in Noda. (2) The level of macropinocytosis of Noda was also smaller than that of conventional Mw cells indicating that the active membrane ruf¯ing of Noda induces rapid recycling. (3) Phagocytosis of opsonized sheep red blood cells (SRBC) was performed more ef®ciently in Noda than in other Mw cell lines. Collectively, these data suggest that in human bone marrow cells, we can identify a unique DC subtype, B/DC line, which develops through a lymphoid DC-differentiation pathway, and DC in this lineage plays an important role in the host immune response because of its effective uptake of a variety of size of antigens by using the skilful membrane ruf¯ing and surface receptors

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“…Ontogenetically, the dendritic cells are considered to develop from the hematopoietic stem cells, and the precursor cells at immature stage spread over the tissue; thus, the differentiation pathway of dendritic cells is distinct from that of monocytes and macrophages [32]. More interestingly, a subset of B cells has been reported to give rise to dendritic morphology and func-tions [4,23]. More interestingly, a subset of B cells has been reported to give rise to dendritic morphology and func-tions [4,23].…”

Section: Introductionmentioning

confidence: 99%

Immunophenotypical changes of neoplastic cells and tumor-associated macrophages in a rat dendritic cell sarcoma-derived transplantable tumor line (KB-D8)

et al. 2003

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Basically, dendritic cell-derived sarcomas are characterized by expression of major histocompatibility complex class-II molecules, but the biological properties of the tumor cells remain to be elucidated. Recently, we established a novel transplantable cell line (KB-D8) from a dendritic cell sarcoma found in an F344 rat. In the present study, we investigated immunophenotypical changes of KB-D8 tumor cells and tumor-associated macrophages (TAMs) appearing in relation to tumor development in syngeneic F344 rats. A number of neoplastic cells in 0.5-cm-diameter KB-D8 tumors showed immunoreactions to OX6 (specific for rat antigen-presenting cells), ED1 (for rat exudate macrophages), and ED2 (for rat resident macrophages), and 72% and 11% of the OX6+ cells were double-immunostained with ED1 and ED2, respectively. Interestingly, the immunoreactions to these antibodies were gradually reduced with increasing size of KB-D8 tumors of 1-, 2-, and 3-cm diameter. These findings indicated that immunophenotypes of dendritic cell-derived sarcomas may be changeable depending on microenvironmental conditions in vivo. Many TAMs seen outside KB-D8 tumors reacted to OX6, ED1, and ED2; the numbers of TAMs immunopositive for these antibodies also decreased as the tumor grew. Similarly, the earlier temporary increase and subsequent gradual decrease in ED2+ and OX6+ cell numbers were observed in the spleen and liver of KB-D8-bearing rats. The reverse-transcription polymerase chain reaction showed mRNA expressions of granulocyte/macrophagecolony stimulating factor, monocyte-chemoattractant protein-1, and osteopontin in KB-D8 tumor tissues. Although the functional roles (biphasic roles: suppressing or promoting) of these factors should be investigated further in relation to tumor development, the factors might be partially responsible for the TAM reactions. KB-D8 would be a useful experimental model to investigate the biological characteristics of dendritic cell sarcomas and tumor immunology in the host.

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A human B‐lineage dendritic cell line, HBM‐Noda and its potential role in human T‐cell leukemia/lymphoma virus type I infection

Cited by 6 publications

References 41 publications

Participation of Different Macrophage Populations and Myofibroblastic Cells in Chronically Developed Renal Interstitial Fibrosis after Cisplatin-induced Renal Injury in Rats

Participation of Different Macrophage Populations and Myofibroblastic Cells in Chronically Developed Renal Interstitial Fibrosis after Cisplatin-induced Renal Injury in Rats

Differential endocytotic characteristics of a novel human B/DC cell line HBM‐Noda: effective macropinocytic and phagocytic function rather than scavenging function

Immunophenotypical changes of neoplastic cells and tumor-associated macrophages in a rat dendritic cell sarcoma-derived transplantable tumor line (KB-D8)

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