A human arterial preparation for studying the effects of vasoactive agents.

Jauernig, RA; Rf, Moulds

doi:10.1161/01.res.42.3.363

Cited by 40 publications

(21 citation statements)

References 15 publications

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“…By the use of suitable antagonists, we have previously shown that NE and 5-HT stimulate separate receptors in digital arteries, 10 Our results do not confirm these previous suggestions, and this study is the first to directly compare detailed dose-response relationships of peripheral and cerebral vessels in humans. The maximum response to 5-HT and the median effective concentration of 5-HT, which define the pharmacological concentration-effect relationship, were not significantly different in the cerebral or peripheral arteries, thus suggesting that the tryptaminergic receptors are probably not pharmacologically different in these 2 vessels.…”

Section: Discussioncontrasting

confidence: 99%

“…Nor is there any clear relationship between the responses and age, sex, cause of delith or prior medication of the patient. 10 This was also found to be the case for cerebral arteries, although there was definite deterioration in some of the vessels removed greater than 30 h after death.…”

Section: Contractile Responses Of Cerebral and Digital Arteriesmentioning

confidence: 65%

“…We have recently investigated the use of postmortem human blood vessels to study the effects of vasoactive agents 10 " 12 and have characterized in detail the responses of human digital arteries to different pharmacological stimuli. It has also been suggested that similarly obtained cerebral blood vessels may be suitable for use as a pharmacological preparation.…”

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confidence: 99%

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Pharmacological comparison of isolated human cerebral and digital arteries.

Rose¹,

Moulds²

1979

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SUMMARY Some basic pharmacological differences between isolated human cerebral and digital arteries obtained at postmortem have been studied. Spiral strip responses were measured isometrically and cumulative drug dose-response curves obtained. Contractile responses were sought to norepinephrine, 5-hydroxytryptamine, angiotensin, histamine, KCI, BaCl 2 and prostaglandin F 2o . Relaxation responses (either by the agonists on their own, or in the presence of a resting tonus induced by KCI or 5-hydroxytryptamine) were sought to isoproterenol, histamine, acetylcholine and dopamine.There were no differences between the cerebral or digital arteries in the non-specific responses to KCI or BaClj. Nor were there any differences in the responses to prostaglandin F 2Q , or in the receptor mediated responses to 5-hydroxytryptamine or angiotensin. This was in marked contrast to the responses produced by norepinephrine where the maximum response was much larger in digital arteries than in cerebral arteries, although there were no differences in the sensitivity of the 2 vessels to norepinephrine (as measured by the ED 60 values). Relaxation responses were difficult to elicit and very variable with only occasional preparations showing relaxation to any of the agonists.These results suggest that the only major pharmacological difference between human digital and cerebral vessels is the number, rather than the sensitivity, of the noradrenergic a-receptors.

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Section: Discussioncontrasting

confidence: 99%

Section: Contractile Responses Of Cerebral and Digital Arteriesmentioning

confidence: 65%

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Pharmacological comparison of isolated human cerebral and digital arteries.

Rose¹,

Moulds²

1979

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“…These values are similar to the ED 50 values reported for other human arteries. 28,29 In the rabbit, the species in which variations in NE sensitivity and receptor affinity have been studied the most, the ED 50 of most arteries is a reasonable approximation of receptor affinity. 30 The NE dose-response curve of the human proximal middle arteries has two components: the first leveling at 1 ϫ10 -5 to 3ϫ10 -5 mol/L.…”

Section: Discussionmentioning

confidence: 99%

Weakness of Sympathetic Neural Control of Human Pial Compared With Superficial Temporal Arteries Reflects Low Innervation Density and Poor Sympathetic Responsiveness

Bevan

Dodge²,

Nichols³

et al. 1998

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Background and Purpose-The primary goal of these studies was to understand and investigate the capacity of perivascular nerves to influence the tone of human pial arteries and to compare them with other human cephalic arteries, the superficial temporal and middle meningeal. Methods-Responses to electrical activation of intramural nerves and related features of fresh segments of human cephalic arteries-the pial (PA; 478Ϯ34 m ID), middle meningeal (MMA; 540Ϯ41 m ID), and superficial temporal (STA; 639Ϯ49 m ID)-obtained from patients aged 15 to 82 years during surgical procedures were studied on a resistance artery myograph. Results-The PA segment responses to electrical nerve activation and to norepinephrine (NE; 10 Ϫ5 mol/L) were 1% and 21% of tissue maximum, respectively, compared with 6% and 34% for the MMA and 14% and 90% for the STA. Tissue maximum was defined as the force increase to 127 mmol/L KCl plus arginine vasopressin (1 m). All arteries dilated well to acetylcholine. Possible explanations for the PA marginal neurogenic responses were assessed. NE ED 50 was 5.4Ϯ2.2ϫ10Ϫ7 mol/L and did not vary with age or diameter. NE responsiveness did not increase in vessels with spontaneous or raised potassium-induced tone. Relaxation to isoproterenol was variable and propranolol did not increase the neurogenic response. Neither N G -monomethyl-L-arginine, N G -nitro-L-arginine methyl ester, endothelium removal, nor indomethacin consistently influenced the contractions to NE or neurogenic reactivity. The weak PA neurogenic response is in keeping with its poor innervation. As determined by catecholamine histofluorescence, innervation in the PA is sparse, with density increasing in the order PA, MMA, and STA. The incidence of nerve structures in the PA adventitio-medial junction was only 3% of those in the STA, and these were situated more than 3 m from the closest smooth muscle cell. Conclusions-We conclude that the weak neurogenic response of adult human pial artery reflects its poor innervation and responsiveness to NE, implying that these features are not important in the regulation of its diameter. (Stroke. 1998;29:212-221.)

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“…It seems reasonable that calcium antagonists are effective on skin vasospastic diseases because of their prop erties to inhibit catecholamine-induced vasoconstrictions (7,8). However, it is reported that calcium antagonists do not act similarly on catecholamine-induced con strictions of various vasculatures but have vascular selectivity (9), and there are few experimental articles available on the in vitro effect of calcium antagonists on the skin supplying arteries (10). We previously reported that the canine intermediate auricular artery, which supplies blood flow mainly to the skin of the ear, represents very similar vascular responsiveness to the human digital artery (11).…”

Section: Calcium Antagonistsmentioning

confidence: 99%